2024-03-28T22:01:10Z
https://u-ryukyu.repo.nii.ac.jp/oai
oai:u-ryukyu.repo.nii.ac.jp:02008859
2023-08-03T05:41:23Z
1642838163960:1642838338003
1642838403551:1642838406414
Biogeography of Persephonella in deep-sea hydrothermal vents of the Western Pacific
Mino, Sayaka
Makita, Hiroko
Toki, Tomohiro
Miyazaki, Junichi
Kato, Shingo
Watanabe, Hiromi
Imachi, Hiroyuki
Watsuji, Tomo-o
Nunoura, Takuro
Kojima, Shigeaki
Sawabe, Tomoo
Takai, Ken
Nakagawa, Satoshi
open access
Copyright © 2013 Mino, Makita, Toki, Miyazaki, Kato, Watanabe, Imachi, Watsuji, Nunoura, Kojima, Sawabe, Takai and Nakagawa. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in other forums, provided the original authors and source are credited and subject to any copyright notices concerning any third-party graphics etc.
population structure
biogeography
deep-sea hydrothermal vent
Persephonellla
Aquificales
MLSA
Deep-sea hydrothermal vent fields are areas on the seafloor with high biological productivity fueled by microbial chemosynthesis. Members of the Aquificales genus Persephone/la are obligately chemosynthetic bacteria, and appear to be key players in carbon, sulfur, and nitrogen cycles in high temperature habitats at deep-sea vents. Although this group of bacteria has cosmopolitan distribution in deep-sea hydrothermal ecosystem around the world, little is known about their population structure such as intraspecific genomic diversity, distribution pattern, and phenotypic diversity. We developed the multi-locus sequence analysis (M LSA) scheme for their genomic characterization. Sequence variation was determined in five housekeeping genes and one functional gene of 36 Persephone/la hydrogeniphila strains originated from the Okinawa Trough and the South Mariana Trough (SNT). Although the strains share >98.7% similarities in 16S rRNA gene sequences, MLSA revealed 35 different sequence types (ST), indicating their extensive genomic diversity. A phylogenetic tree inferred from all concatenated gene sequences revealed the clustering of isolates according to the geographic origin. In addition, the phenotypic clustering pattern inferred from whole-cell matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF/MS) analysis can be correlated to their MLSA clustering pattern. This study represents the first MLSA combined with phenotypic analysis indicative of allopatric speciation of deep-sea hydrothermal vent bacteria.
論文
Frontiers Media
2013-02-28
eng
journal article
VoR
http://hdl.handle.net/20.500.12000/37687
http://hdl.handle.net/20.500.12000/37687
https://u-ryukyu.repo.nii.ac.jp/records/2008859
https://doi.org/10.3389/fmicb.2013.00107
info:doi/10.3389/fmicb.2013.00107
1664-302X
Frontiers in microbiology
4
107
none
https://u-ryukyu.repo.nii.ac.jp/record/2008859/files/fmicb-04-00107.pdf