2024-03-29T02:16:04Z
https://u-ryukyu.repo.nii.ac.jp/oai
oai:u-ryukyu.repo.nii.ac.jp:02010329
2023-08-03T05:31:08Z
1642838163960:1642838338003
1642838403551:1642838406414
核DNAマーカーによるミナミメダカ沖縄諸島タイプの 確率的判定と低い遺伝的多様性
Conservation implications of low genetic diversity of the unique Okinawa Islands subpopulation of Oryzias latipes based on nuclear DNA markers
畑中, ひらり
今井, 秀行
Hatanaka, Hirari
Imai, Hideyuki
open access
This study included 508 individuals of Oryzias latipes from 15 sites in mainland Japan and 10 sites in the Ryukyu Archipelago and Taiwan used in Imai et al. (2017), and new samples from four sites in the Ryukyu Archipelago, four sites in China and South Korea, and one from hi-medaka. We developed four new PCR-RFLP nuclear DNA markers (Orla16- 185-MboI, Orla1-29-BstUI, Orla1-51-AluI, and P0Ex-HinfI) based on three universal microsatellite primer sets and an exon-primed intron-crossing (EPIC) primer set for fish. STRUCTURE analysis indicated that Amami-oshima, Iheya, Izena, Ginama, Ogimi, and Bios no Oka formed a cluster, while specimens from Fukuchi, the former Kadena Ammunition Storage area, Zamani, and other Okinawa Islands formed a different cluster. The genetic diversity of subpopulations from Fukuchi, the former Kadena Ammunition Storage area, and Zamani was higher than those from other Okinawa Islands. The individual exclusion test (>90% condition) was applied to the Okinawa Islands subpopulation using GeneClass2. It will be important to apply these genetic markers for periodical genetic monitoring of this subpopulation.
論文
日本生物地理学会
The Biogeographical Society of Japan
2018-12-20
jpn
journal article
VoR
http://hdl.handle.net/20.500.12000/43516
http://hdl.handle.net/20.500.12000/43516
https://u-ryukyu.repo.nii.ac.jp/records/2010329
0067-8716
AN00193863
日本生物地理学会会報
Bulletin of the Bio-geographical Society of Japan
73
60
69
https://u-ryukyu.repo.nii.ac.jp/record/2010329/files/hatanaka_imai_2018.pdf