2024-03-29T08:33:23Z
https://u-ryukyu.repo.nii.ac.jp/oai
oai:u-ryukyu.repo.nii.ac.jp:02011262
2023-08-03T05:27:13Z
1642838163960:1642838338003
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A Novel Ganglioside Isolated from Renal Cell Carcinoma
Ito, Akihiro
Levery, Steven B.
Saito, Seiichi
Satoh, Makoto
Hakomori, Sen-itiroh
open access
In renal cell carcinoma (RCC), the level of higher gangliosides is correlated with degree of metastatic potential, and cell lines derived from metastatic deposits of RCC are characterized by high expression of disialogangliosides (Saito, S., Orikasa, S., Ohyama, C., Satoh, M., and Fukushi, Y. (1991) Int. J. Cancer 49, 329–334 and Saito, S., Orikasa, S., Satoh, M., Ohyama, C., Ito, A., and Takahashi, T. (1997) Jpn. J. Cancer Res. (Gann) 88, 652–659). We now report two disialogangliosides, G1 and G2, found in the RCC cell line TOS-1. G1 from TOS-1 cells was characterized as having a novel hybrid structure between ganglio-series (region I as in Structure FTI; same as the terminal structure of ganglioside GM2), and the lacto-series type 1 (region II). The characterization was based on reactivity with various monoclonal antibodies (mAbs) with defined epitope specificity, as well as monosaccharide and fatty acid component analysis, ^1H NMR spectroscopy, and electrospray ionization mass spectrometry of the intact compound. G1 showed strong reactivity with mAb RM2, raised originally against TOS-1 cells, and weak cross-reactivity with anti-GM2 mAb MK-1–8. The antigen is hereby termed GalNAc disialosyl Lc_4Cer (IV^4GalNAcIV^3NeuAcIII^6NeuAcLc_4; abbreviated GalNAcDSLc_4). G2 was identified by^1H NMR and mass spectrometry as having a structure similar to Structure FTI but without the GalNAcβ1→4 substitution and showed strong reactivity with mAb FH9 reported previously to be specific for disialosyl lacto-series type 1 (disialosyl Lc_4) having vicinal α2→3 and α2→6 sialosyl residues, an antigen associated with human colonic cancer. Clinicopathological studies indicate that expression of these disialoganglioside antigens in RCC tissue is correlated with the metastatic potential of RCC.
論文
American Society for Biochemistry and Molecular Biology
2001-05-18
eng
journal article
VoR
http://hdl.handle.net/20.500.12000/47350
http://hdl.handle.net/20.500.12000/47350
https://u-ryukyu.repo.nii.ac.jp/records/2011262
https://doi.org/10.1074/jbc.M011791200
https://doi.org/10.1074/jbc.M011791200
1083-351X
0021-9258
Journal of Biological Chemistry
276
20
16695
16703
https://u-ryukyu.repo.nii.ac.jp/record/2011262/files/J. Biol. Chem.-2001-Ito-16695-703.pdf