2024-03-29T06:31:33Z
https://u-ryukyu.repo.nii.ac.jp/oai
oai:u-ryukyu.repo.nii.ac.jp:02011307
2023-08-03T05:32:56Z
1642838163960:1642838338003
1642838403551:1642838407795
Leptospiral outer membrane protein LMB216 is involved in enhancement of phagocytic uptake by macrophages
Toma, Claudia
Murray, Gerald L.
Nohara, Toshitsugu
Mizuyama, Masaru
Koizumi, Nobuo
Adler, Ben
Suzuki, Toshihiko
open access
Leptospira interrogans is responsible for the zoonotic disease leptospirosis. The pathogenic mechanisms of this spirochaete remain poorly understood; however, virulence has been correlated with increased phagocytic uptake and survival within macrophages. Leptospiral outer membrane proteins are thought to be responsible for persistence in vivo via interaction with specific host components. In this study, we analysed the transcriptional profile of a virulent strain and its culture‐attenuated derivative strain to identify bacterial factors that may be involved in pathogenesis. Two outer membrane proteins, LMB216 and LigB (leptospiral immunoglobulin‐like protein B) were downregulated more than 10‐fold in the culture‐attenuated strain. We show that both proteins play a role in leptospiral uptake by macrophages and that LMB216, as well as LigB, enhances the binding of leptospires to fibronectin. Taken together, our results indicate that LMB216 plays a role in pathogen interaction with host molecule/s, which may contribute to pathogenesis of leptospirosis.
論文
Wiley
2014-09
eng
journal article
AM
http://hdl.handle.net/20.500.12000/47485
http://hdl.handle.net/20.500.12000/47485
https://u-ryukyu.repo.nii.ac.jp/records/2011307
https://doi.org/10.1111/cmi.12296
https://doi.org/10.1111/cmi.12296
1462-5822
1462-5814
Cellular Microbiology
16
9
1366
1377
https://u-ryukyu.repo.nii.ac.jp/record/2011307/files/Toma et al. 2014_ for UR.pdf