2024-03-29T05:48:38Z
https://u-ryukyu.repo.nii.ac.jp/oai
oai:u-ryukyu.repo.nii.ac.jp:02012419
2023-08-03T05:36:28Z
1642838163960:1642838338003
1642838403551:1642838407795
MicroRNA-196a-5p is a potential prognostic marker of delayed lymph node metastasis in early-stage tongue squamous cell carcinoma
Maruyama, Tessho
Nishihara, Kazuhide
Umikawa, Masato
Arasaki, Akira
Nakasone, Toshiyuki
Nimura, Fumikazu
Matayoshi, Akira
Takei, Kimiko
Nakachi, Saori
Kariya, Ken-Ichi
Yoshimi, Naoki
open access
Creative Commons Attribution-NonCommercial-NoDerivatives 4.0
https://creativecommons.org/licenses/by-nc-nd/4.0/
microRNA
tongue
oral cavity
squamous cell carcinoma
miR-196a
lymph node metastasis
regional recurrence
prognostic marker
MicroRNAs (miRs) are expected to serve as prognostic tools for cancer. However, many miRs have been reported as prognostic markers of recurrence or metastasis in oral squamous cell carcinoma patients. We aimed to determine the prognostic markers in early‑stage tongue squamous cell carcinoma (TSCC). Based on previous studies, we hypothesized that miR‑10a, 10b, 196a‑5p, 196a‑3p, and 196b were prognostic markers and we retrospectively performed miR expression analyses using formalin‑fixed paraffin‑embedded sections of surgical specimens. Total RNA was isolated from cancer tissues and adjacent normal tissue as control, and samples were collected by laser‑capture microdissection. After cDNA synthesis, reverse transcription‑quantitative polymerase chain reaction was performed. Statistical analyses for patient clinicopathological characteristics, recurrence/metastasis, and survival rates were performed to discern their relationships with miR expression levels, and the 2‑ΔΔCq method was used. miR‑196a‑5p levels were significantly upregulated in early‑stage TSCC, particularly in the lymph node metastasis (LNM) group. The LNM‑free survival rate in the low miR‑196a‑5p ΔΔCq value regulation group was found to be lower than that in the high ΔΔCq value regulation group (P=0.0079). Receiver operating characteristic analysis of ΔΔCq values revealed that miR‑196a‑5p had a P‑value=0.0025, area under the curve=0.740, and a cut‑off value=‑0.875 for distinguishing LNM. To our knowledge, this is the first study to examine LNM‑related miRs in early‑stage TSCC as well as miRs and ‘delayed LNM’ in head and neck cancer. miR‑196a‑5p upregulation may predict delayed LNM. Our data serve as a foundation for future studies to evaluate miR levels and facilitate the prediction of delayed LNM during early‑stage TSCC, which prevent metastasis when combined with close follow‑up and aggressive adjuvant therapy or elective neck dissection. Moreover, our data will serve as a foundation for future studies to evaluate whether miR‑196a‑5p can serve as a therapeutic marker for preventing metastasis.
論文
Spandidos Publications
2018-01
eng
journal article
VoR
http://hdl.handle.net/20.500.12000/45982
http://hdl.handle.net/20.500.12000/45982
https://u-ryukyu.repo.nii.ac.jp/records/2012419
https://doi.org/10.3892/ol.2017.7562
https://doi.org/10.3892/ol.2017.7562
1792-1074
1792-1082
Oncology Letters
15
2
2349
2363
https://u-ryukyu.repo.nii.ac.jp/record/2012419/files/ol_15_2_2349_PDF.pdf