2024-03-28T16:23:18Z
https://u-ryukyu.repo.nii.ac.jp/oai
oai:u-ryukyu.repo.nii.ac.jp:02012535
2023-08-03T05:31:15Z
1642838163960:1642838338003
1642838403551:1642838407795
Sequence Analysis of a Total of Three Megabases of DNA in Two Regions of Chromosome 8p
Isomura, Minoru
Ikegawa, Shiro
Kinjo, Takao
Takeuchi, Kumiko
Yamane-Tanaka, Yuka
Kitami, Kikuko
Nakamura, Yusuke
open access
large-scale DNA sequencing
physical and transcriptional maps
human chromosome 8p11.2
human chromosome 8p22→p21.3
Large-scale sequencing of genomic regions and in silico gene trapping together represent a highly efficient and powerful approach for identifying novel genes. We performed megabase-level sequence analyses of two genomic regions on human chromosome 8p (8p11.2 and 8p22.→p21.3), after covering those segments with sequence-ready contigs composed of 74 cosmids, 14 BACs, and three PAC clones. We determined continuous nucleotide sequences of 1,856,753 bases on 8p11.2 and 1,210,381 bases on 8p22→p21.3 by combining the shotgun and primer-walking methods. In silico gene trapping identified four novel genes in the 8p11.2 region and, in the 8p22→p21.3 region, six known genes ( PRLTS, PCM1, MTMR7, HCAT2, HFREP-1 and PHP ) and three novel genes. The distribution of Alu and LINE1 repetitive elements and the densities of predicted exons were different in each region, and Alu -rich portions contained more exonic sequences than LINE1-rich areas.
論文
Oxford University Press
1999-12-01
eng
journal article
VoR
http://hdl.handle.net/20.500.12000/46345
http://hdl.handle.net/20.500.12000/46345
https://u-ryukyu.repo.nii.ac.jp/records/2012535
https://doi.org/10.1093/dnares/6.6.387
https://doi.org/10.1093/dnares/6.6.387
1340-2838
1756-1663
DNA Research
6
6
387
400
https://u-ryukyu.repo.nii.ac.jp/record/2012535/files/dnares_6_6_387.pdf