2024-03-28T14:13:11Z
https://u-ryukyu.repo.nii.ac.jp/oai
oai:u-ryukyu.repo.nii.ac.jp:02016118
2022-10-31T07:50:46Z
1642838163960:1642838198944:1642838199408:1642838209529
1642838403551:1642838412624
[原著]Graft-directed gene transfection in a rat model of liver transplantation
Shiraishi, Masayuki
Hiroyasu, Shungo
Tomori, Hirohumi
Ma, Shao-ping
Makino, Yoshihiro
Kusano, Toshiomi
Muto, Yoshihiro
open access
琉球医学会
gene transfer
adenovirus
retrovirus
liver transplantation
In this model of liver transplantation, liver grafts were transfected with the El-deleted, replication-defective adenovirus vectors encoding the LacZ gene driven by the human CMV promoter, or the replication-defective retrovirus vectors encoding the human IL-7 and neomycin phosphotransferase genes. Liver grafts were perfused ex-vivo with either of these two vectors during cold preservation time. In adenovirus-mediated gene transfer, positive staining of the hepatocyte was recognized predominantly around the portal triads at post-operative day 2. Positive staining was observed until 14 days after transfection. In a retrovirus-mediated gene transfection, gene transfection was tested both in a whole-size and a reduced-size graft implantation of the liver because the retrovirus vector requires a replication of the target cells for gene transfection. In conclusion, adenovirus-mediated gene transfection to the liver graft during cold preservation time resulted in an effective expression of the transfected sequence. On the other hand, retrovirus vector required the reduction of the liver graft for a successful gene transfection.
論文
琉球医学会
Ryukyu Medical Association
1997
eng
journal article
VoR
http://hdl.handle.net/20.500.12000/0002016118
https://u-ryukyu.repo.nii.ac.jp/records/2016118
1346888X
0289-1530
AN10369445
琉球医学会誌 = Ryukyu Medical Journal
17
1
21
24
https://u-ryukyu.repo.nii.ac.jp/record/2016118/files/v17p21.pdf