2024-03-29T10:26:52Z
https://u-ryukyu.repo.nii.ac.jp/oai
oai:u-ryukyu.repo.nii.ac.jp:02016140
2022-10-31T07:51:11Z
1642838163960:1642838198944:1642838199408:1642838216134
1642838403551:1642838412624
[原著]An improved method for the detection of IgE FE-3 by ELISA using monoclonal anti-IgE FE-3 antibody
Nakasone,Toshiyuki
Hanashiro, Kazuhiko
Nakamura, Mariko
Sunakawa, Hajime
Kosugi, Tadayoshi
open access
琉球医学会
monoclonal antibody against rat IgE
rat IgE
DNP-specific rat IgE
IgE-capture ELISA
A hybridoma producing monoclonal antibody against monoclonal DNP-specific rat IgE (IgE FE-3) was generated by fusion of P3-X63-Ag8-Ul (P3U1) mouse myeloma and spleen cells from BALB/c mice immunized with IgE FE-3. Five hybridoma clones were obtained. Three clones of them had a high reactivity against IgE FE-3 and a low reactivity against the L-chain of IgE FE-3 on ELISA analysis for the first screening of the hybridoma. The binding of monoclonal antibodies produced from clone BB-9 to IgE FE-3 revealed the strongest inhibition among three monoclonal antibodies on pretreatment with polyclonal rabbit anti-IgE FE-3 antibodies. It was clarified that monoclonal antibody BB-9 reacted specifically with the heavy chain of IgE FE-3 by Western blotting analysis. Furthermore, the reactivity of the monoclonal antibody BB-9 to IgE FE-3 was higher than that of MARE-1, which is a monoclonal antibody against rat myeloma IgE. The monoclonal antibody BB-9 was therefore employed for IgE-capture ELISA to determine the concentration of IgE FE-3. No significant alterations of color development occurred when igE FE-3 was concomitantly incubated with large excesses of IgG antibodies and rat myeloma IgE. Our data indicated that IgE-capture ELISA employing the monoclonal antibody BB-9 had a higher sensitivity for the determination of IgE FE-3 than did that using polyclonal rabbit anti-IgE FE-3 antibodies.
論文
琉球医学会
Ryukyu Medical Association
2001
eng
journal article
VoR
http://hdl.handle.net/20.500.12000/0002016140
https://u-ryukyu.repo.nii.ac.jp/records/2016140
1346888X
0289-1530
AN10369445
琉球医学会誌 = Ryukyu Medical Journal
20
3
129
135
https://u-ryukyu.repo.nii.ac.jp/record/2016140/files/v20p129.pdf