2024-03-29T09:17:28Z
https://u-ryukyu.repo.nii.ac.jp/oai
oai:u-ryukyu.repo.nii.ac.jp:02016345
2022-02-22T04:55:24Z
1642838163960:1642838198944:1642838242961:1642838245041
1642838403551:1642838412624
[原著]Deoxyribonuclease Iによる核酸初期分解生成物とb-form核酸との比較
Comparison of a b-form nucleic acid with an initial degradation product produced by deoxyribonuclease I
井上, 文英
中田, 福市
Inoue, Fumihide
Nakada, Fukuichi
open access
琉球医学会
The purpose of this paper is to elucidate the hydrolytic processes and the physicochemical properties of the initial hydrolysate of thymus DNA by DNase I (EC-3.1.4.5) in the presence of a very low concentration of Mg^<2+>. By examining the increasing curve on the acid soluble material and hyperchromicity, the hydrolytic process of DNA was limited by DNase I under very low Mg^<2+>concentration. The partially hydrolyzed product (ph-DNA) did not show any spectral changes by the disruption of hydrogen bonds involving the reactive amino acid. Therefore, ph-DNA is considered to still retain a double helical structure. ph-DNA was obtained from the reaction mixture under hydrolytic condition and when more Mg^<2+> was added to the reaction mixture, the b-form DNA was produced. It may be concluded that the ph-DNA is the initial degradation product of DNA obtainable by the action of DNase I in the presence of trace Mg^<2+>.
論文
琉球大学保健学部
College of Health Sciences, University of the Ryukyus
jpn
journal article
VoR
http://hdl.handle.net/20.500.12000/0002016345
https://u-ryukyu.repo.nii.ac.jp/records/2016345
0285-9270
AN00250763
琉球大学保健学医学雑誌=Ryukyu University Journal of Health Sciences and Medicine
1
4
296
300
https://u-ryukyu.repo.nii.ac.jp/record/2016345/files/v1p296.pdf