2024-03-29T07:28:24Z
https://u-ryukyu.repo.nii.ac.jp/oai
oai:u-ryukyu.repo.nii.ac.jp:02016428
2022-02-22T04:57:51Z
1642838163960:1642838198944:1642838242961:1642838244566
1642838403551:1642838412624
[原著]ウェルシュ菌 K29 株の産生するバクテリオシンおよび溶菌物質の精製とその性状
Partial purification and some properties of the bacteriocin and the bacteriolytic substance produced by Clostridium perfringens strain K29
吉元, 房枝
今村, 禎祐
Yoshimoto, Fusae
Imamura, Teisuke
open access
琉球医学会
Substances inhibiting the growth of sensitive cells and lysing them, produced by Clostridium perfringens strain K29 were separated in high purity by 50% ammonium sulfate precipitation, gel filtration on Sephadex G-100 and ion exchange chromatography on DEAE Sephadex A-50. The two substances obtained were compared for physicochemical properties and the effect on sensitive cells. At the range from pH 7 to 8, the inhibitory substance was stable, whereas the bacteriolytic substance was stable at the range from pH 5 to 8. Both substances were inactivated by proteinase and by heat treatment for 5 minutes at 50℃. The effect of the inhibitory substance appeared to be bactericidal but not bacteriolytic, whereas the bacteriolytic substance lysed sensitive cells rapidly. According to this investigation, it is satisfactory to consider the former as a bacteriocin and the latter as a lytic enzyme.
論文
琉球大学保健学部
College of Health Sciences, University of the Ryukyus
jpn
journal article
VoR
http://hdl.handle.net/20.500.12000/0002016428
https://u-ryukyu.repo.nii.ac.jp/records/2016428
0285-9270
AN00250763
琉球大学保健学医学雑誌=Ryukyu University Journal of Health Sciences and Medicine
1
3
185
197
https://u-ryukyu.repo.nii.ac.jp/record/2016428/files/v1p185.pdf