2024-03-29T07:12:19Z
https://u-ryukyu.repo.nii.ac.jp/oai
oai:u-ryukyu.repo.nii.ac.jp:02002905
2022-10-31T01:11:22Z
1642837622505:1642837905044:1642837916513
1642838403551:1642838407312
バガスの酵素分解性とフェノール化合物(農芸化学科)
Enzymatic degradation of sugar-cane bagasse and phenolic compounds (Department of Agricultural Chemistry)
石原, 昌信
石垣, 勝己
当山, 清善
Ishihara, Masanobu
Ishigaki, Katsumi
Toyama, Seizen
粉砕バガスの酵素分解反応を行ない,酵素分解生成物の紫外部吸収値とフェノール化合物について調べた。酵素分解生成物をNaOH溶液で前処理すると280nm吸収値が著しく増加した。NaOH処理した酵素分解生成物にはフェルラ酸以外のフェノール化合物は認められなかった。酵素分解生成物中のフェルラ酸をペーパークロマトグラフィーにより他成分と分離するには試料をNaOHで前処理する必要があった。バガス中のフェノール化合物量とバガスの酵素分解性との間には相関性が認められた。酵素分解生成物はゲル濾過により高分子及び低分子画分に分画され,両画分にはフェルラ酸と糖が存在した。高分子画分の糖成分はグルコース,キシロース及びアラビノースであった。
The ultraviolet absorbance of the supernatant and the water soluble phenolic compounds released were determined after treating sugar-cane bagasse with a commercial cellulase preparation,Meicelase. When the supernatant was treated with NaOH solution at 120℃ for 30min, the absorption value at 280nm was increased. Ferulic acid, the only phenolic compound detected, was extracted with ethylacetate from the supernatant which was treated with NaOH. Ferulic acid which was released from bagasse during enzyme reaction could be separated from the other substances by paperchromatography. when sugar-cane bagasse was treated with NaOH, the degradability of the residues by cellulase preparation was strongly correlated with the amount of phenolic compound released by the alkali. Gel filtration of the enzyme hydrolysate on Toyopear HW 40 gave low-and high-molecular-weight fractions, both of which appeared to contain phenolic-complexes. The high-molicular-weight fraction obtained by gel filtration contained glucose, xylose and arabinose.
紀要論文
http://purl.org/coar/resource_type/c_6501
琉球大学農学部
1989-12-05
VoR
http://hdl.handle.net/20.500.12000/3876
0370-4246
AN00250548
琉球大学農学部学術報告
The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus
36
65
59
jpn
open access