2024-03-29T02:25:29Z
https://u-ryukyu.repo.nii.ac.jp/oai
oai:u-ryukyu.repo.nii.ac.jp:02004072
2023-08-03T05:25:16Z
1642838163960:1642838338003
1642838403551:1642838407795
Human T-cell leukemia virus type I infects human lung epithelial cells and induces gene expression of cytokines, chemokines and cell adhesion molecules
Teruya, Hiromitsu
Tomita, Mariko
Senba, Masachika
Ishikawa, Chie
Tamayose, Maki
Miyazato, Akiko
Yara, Satomi
Tanaka, Yuetsu
Iwakura, Yoichiro
Fujita, Jiro
Mori, Naoki
[Background] Human T-cell leukemia virus type I (HTLV-I) is associated with pulmonary diseases, characterized by bronchoalveolar lymphocytosis, which correlates with HTLV-I proviral DNA in carriers. HTLV-I Tax seems to be involved in the development of such pulmonary diseases through the local production of inflammatory cytokines and chemokines in T cells. However, little is known about induction of these genes by HTLV-I infection in lung epithelial cells.
[Results] We tested infection of lung epithelial cells by HTLV-I by coculture studies in which A549 alveolar and NCI-H292 tracheal epithelial cell lines were cocultured with MT-2, an HTLV-I-infected T-cell line. Changes in the expression of several cellular genes were assessed by reverse transcription-polymerase chain reaction, enzyme-linked immunosorbent assay and flow cytometry. Coculture with MT-2 cells resulted in infection of lung epithelial cells as confirmed by detection of proviral DNA, HTLV-I Tax expression and HTLV-I p19 in the latter cells. Infection was associated with induction of mRNA expression of various cytokines, chemokines and cell adhesion molecule. NF-κB and AP-1 were also activated in HTLV-I-infected lung epithelial cells. In vivo studies showed Tax protein in lung epithelial cells of mice bearing Tax and patients with HTLV-I-related pulmonary diseases.
[Conclusion] Our results suggest that HTLV-I infects lung epithelial cells, with subsequent production of cytokines, chemokines and cell adhesion molecules through induction of NF-κB and AP-1. These changes can contribute to the clinical features of HTLV-I-related pulmonary diseases.
論文
http://purl.org/coar/resource_type/c_6501
BioMed Central Ltd.
2008-09-22
AM
http://hdl.handle.net/20.500.12000/7347
1742-4690
AA12051445
Retrovirology
5
86
eng
http://www.retrovirology.com/content/5/1/86
10.1186/1742-4690-5-86
18808681
open access