2024-03-29T13:39:44Z
https://u-ryukyu.repo.nii.ac.jp/oai
oai:u-ryukyu.repo.nii.ac.jp:02006988
2022-10-31T03:12:49Z
1642837622505:1642837994858:1642837999752
1642838403551:1642838406414
The Isolation of Photoactive Chloroplasts from Leaves of the Mangrove, Kondelio condel Druce
Ikehara, Norikatsu
Sashida, Fusako
Soeishi, Takako
A method for isolating photoactive chloroplasts from mangrove leaves was investigated. Chloroplasts with the Hill reaction activity(photoreduction of ferricyanide) were isolated from the leaves of a mangrove plant, Kandelia candel Druce, by employing PEG in the grinding medium. Some characteristics of these chloroplasts are presented in this paper. Grinding medium for isolating photoactive chloroplasts is consisted of 1M sucrose,10%(W/V) PEG 4000, 0.025 M sodium iso-ascorbate, 0.05 M magnesium chloride and 0.05 M histidine buffered at pH 6.5 with HCI. Chloroplasts isolated without PEG reduces ferricyanide considerably in the dark. However, ferricyanide reduction in the dark was decreased by addition of PEG in the grinding medium. A possibility of tannins as the reductant is examined.
紀要論文
http://purl.org/coar/resource_type/c_6501
琉球大学理工学部
Science and Engineering Division, University of the Ryukyus
1975-09-01
NA
http://hdl.handle.net/20.500.12000/23533
AN0025080X
琉球大学理工学部紀要. 理学編
Bulletin of Science & Engineering Division, University of Ryukyus. Mathematics & natural sciences
20
59
51
eng
open access