2024-03-28T13:38:57Z
https://u-ryukyu.repo.nii.ac.jp/oai
oai:u-ryukyu.repo.nii.ac.jp:02011302
2023-08-03T05:31:06Z
1642838163960:1642838338003
1642838403551:1642838407795
Roles of GalNAc-disialyl Lactotetraosyl Antigens in Renal Cancer Cells
Tsuchida, Akio
Senda, Motohiro
Ito, Akihiro
Saito, Seiichi
Kiso, Makoto
Ando, Takayuki
Harduin-Lepers, Anne
Matsuda, Akio
Furukawa, Keiko
Furukawa, Koichi
GalNAc-disialyl Lc4 (GalNAc-DSLc4) was reported as a novel antigen that associated with malignant features of renal cell cancers (RCCs). To clarify roles of GalNAc-DSLc4 in malignant properties of RCCs, we identified B4GalNAc-T2 as a responsible gene for the synthesis of GalNAc-DSLc4, and prepared stable transfectants of GalNAc-T2 cDNA using VMRC-RCW cells, resulting in the establishment of high expressants of GalNAc-DSLc4. They showed increased proliferation and invasion, and specific adhesion to laminin. In the transfectants, PI3K/Akt signals were highly activated by serum stimulation or adhesion to laminin. GalNAc-DSLc4 was co-localized in lipid rafts with integrin β1 and caveolin-1 in both immunoblotting of fractionated detergent extracts and immunocytostaining, particularly when stimulated with serum. Masking of GalNAc-DSLc4 with antibodies as well as PI3K inhibitor suppressed malignant properties of the transfectants. These results suggested that GalNAc-DSLc4 is involved in malignant properties of RCCs by forming a molecular complex with integrins in lipid rafts.
論文
http://purl.org/coar/resource_type/c_6501
Springer Nature
2018-05-04
VoR
http://hdl.handle.net/20.500.12000/47490
2045-2322
Scientific Reports
8
eng
https://doi.org/10.1038/s41598-018-25521-6
https://doi.org/10.1038/s41598-018-25521-6
open access
Creative Commons Attribution 4.0
https://creativecommons.org/licenses/by/4.0/