2024-03-29T14:19:14Z
https://u-ryukyu.repo.nii.ac.jp/oai
oai:u-ryukyu.repo.nii.ac.jp:02015649
2022-10-31T07:41:51Z
1642838163960:1642838308020:1642838308479:1642838331350
1642838403551:1642838412624
イモゾウムシの卵接種が起因となる人工幼虫飼料汚染の防止
Prevention of contamination on the artificial larval diet of West Indian sweetpotato weevil Euscepes postfasciatus (Fairmaire) from their inoculated eggs.
下地, 幸夫
Shimoji, Yukio
イモゾウムシ
人工飼料
不妊虫飼育
殺菌処理
汚染
バクテリア
卵表面殺菌液
Prevention of the bacterial contamination on the artificial larval diet of West Indian sweetpotato weevil Eucepes postfasciatus (Fairmaire) was examined. Bacteria on the surface of eggs-inoculated on diet was considered as a source of diet contamination. The surface of eggs were rinsed with 70% ethanol solution several times as follows; a half, 1, 2, and 5 minutes. Surface-sterilized eggs for 30 seconds did not permit the emergence of bacteria, indicating the necessity of sterilization of eggs more than 30 seconds to prevent the contamination of bacteria into an artificial larval diet. Eggs were then transferred to artificial larval diet after they were dipped in 5% formaldehyde solution to sterilize for 20 minutes. The dipping treatment could significantly suppress the emergence of bacteria for a long period and produced significantly more surviving weevils from artificial larval diet than no treatment control diet.
論文
http://purl.org/coar/resource_type/c_6501
沖縄農業研究会
Okinawa Agriculture Research Society
2003-07
VoR
1344-1477
AN00083297
沖縄農業
1
37
60
57
jpn
open access
沖縄農業研究会