2024-03-29T09:46:57Z
https://u-ryukyu.repo.nii.ac.jp/oai
oai:u-ryukyu.repo.nii.ac.jp:02015854
2022-10-31T07:45:45Z
1642838163960:1642838198944:1642838199408:1642838200917
1642838403551:1642838412624
[原著]Increased Susceptibility of B-cells in Protein-Calorie Malnutrition to Long Term Low Dose Streptozotocin and Its Effect on Re-feeding
Islam, Mohammad Rashidul
Murakami, Keiji
WakugamiTamio
Mimura, Goro
Streptozotocin
Nutrition disorders
Islet of Langerhans
Protein-calorie malnutrition
The susceptibility of pancreatic B-cells of protein-calorie malnourished(PCM) rats to long term low dose streptozotocin(STZ) and the effect of this low dose STZ on refeeding were studied. Male weanling Wistar rats were fed either by 20%(30=normal) or 4%(30=PCM) protein diet. Twenty of each two groups were treated with STZ(5mg/kg/day i.m.) and the rest with buffer for one month. Ten of each STZ treated normal and PCM rats were re-fed with 20% protein diet for another one month called as RF-normal and RF-PCM respectively. Wet wt. of liver and pancreas, plasma lipids and insulin were measured. IPGTT, morphometric and histologic study of islets were done. PCM+STZ showed significantly high fasting plasma glucose (FPG) $175.9 \pm 46.6$ compared to $88.8 \pm 12.9$mg/dl ($mean \pm SD$) in normal+STZ rats. Both STZ and vehicle treated PCM rats showed impaired IPGTT with peak plasma glucose $663.4 \pm 87.8$ and $465.8 \pm 97.2$mg/dl ($mean \pm SD$) respectively. Areas under curves in case of IPGTT were significantly high in PCM with and without STZ than in normal with and without S12. These were also significantly high in normal and PCM with STZ, compared to normal and PCM without STZ. No./field and size of islet, % of B-cell area/islet and insulin levels were significantly reduced (pくO.01 vs. normal) in both STZ and vehicle treated PCM rats. Degree of reduction was more prominent in PCM+STZ. FPG remained high with $221.0 \pm 98.8$mg/dl despite some nutritional recovery in RF-PCM rats. Areas under curves in case of IPGTT were significantly high in RF-PCM than RF-normal in both before and after refeeding. The area under curve reduced significantly in RF-normal after refeeding but it was unchanged in RF-PCM rats. RF-PCM rats also showed significant reduction in morphometry and insulin level (p<0.01 vs. RF-nomal) and degree of reduction was more in RE-PCM after re-feeding. Histologic findings showed close correlationship with morphometry. We concluded that B-cel1s of morphologinIly altered islets of PCM rats were more susceptible to STZ and the effect of STZ was persistent after refeeding. These suggest the possibility of increased susceptibility of pancreatic B-cells to other B-cytotoxic influences as STZ in PCM in childhood and exposure to these agents might produce an irreversible change to the endocrine pancreas.
論文
http://purl.org/coar/resource_type/c_6501
琉球医学会
Ryukyu Medical Association
1992
VoR
1346888X
0289-1530
AN10369445
琉球医学会誌 = Ryukyu Medical Journal
3
12
218
206
eng
open access
琉球医学会