2024-03-28T14:02:09Z
https://u-ryukyu.repo.nii.ac.jp/oai
oai:u-ryukyu.repo.nii.ac.jp:02016345
2022-02-22T04:55:24Z
1642838163960:1642838198944:1642838242961:1642838245041
1642838403551:1642838412624
[原著]Deoxyribonuclease Iによる核酸初期分解生成物とb-form核酸との比較
Comparison of a b-form nucleic acid with an initial degradation product produced by deoxyribonuclease I
井上, 文英
中田, 福市
Inoue, Fumihide
Nakada, Fukuichi
The purpose of this paper is to elucidate the hydrolytic processes and the physicochemical properties of the initial hydrolysate of thymus DNA by DNase I (EC-3.1.4.5) in the presence of a very low concentration of Mg^<2+>. By examining the increasing curve on the acid soluble material and hyperchromicity, the hydrolytic process of DNA was limited by DNase I under very low Mg^<2+>concentration. The partially hydrolyzed product (ph-DNA) did not show any spectral changes by the disruption of hydrogen bonds involving the reactive amino acid. Therefore, ph-DNA is considered to still retain a double helical structure. ph-DNA was obtained from the reaction mixture under hydrolytic condition and when more Mg^<2+> was added to the reaction mixture, the b-form DNA was produced. It may be concluded that the ph-DNA is the initial degradation product of DNA obtainable by the action of DNase I in the presence of trace Mg^<2+>.
論文
http://purl.org/coar/resource_type/c_6501
琉球大学保健学部
College of Health Sciences, University of the Ryukyus
VoR
0285-9270
AN00250763
琉球大学保健学医学雑誌=Ryukyu University Journal of Health Sciences and Medicine
4
1
300
296
jpn
open access
琉球医学会