2024-03-29T08:16:38Z
https://u-ryukyu.repo.nii.ac.jp/oai
oai:u-ryukyu.repo.nii.ac.jp:02019640
2023-08-03T05:37:47Z
1642838163960:1642838338003
1642838403551:1642838407795
Evaluation of Islet Purification Methods for Making a Continuous Density Gradient and Loading Tissue
Ebi, Nana
Miyagi-Shiohira, Chika
Hamada, Eri
Tamaki, Yoshihito
Masamoto, Mariko
Makishi, Erika
Nakashima, Yoshiki
Kobayashi, Naoya
Saitoh, Issei
Watanabe, Masami
Noguchi, Yasufumi
Kinjo, Takao
Noguchi, Hirofumi
islet transplantation
islet isolation
islet purification
bottle purification
continuous density gradient
Islet purification is one of the most important steps of islet isolation for pancreatic islet transplantation. We previously reported that a purification method using large plastic bottles effectively achieved a high yield of islets from porcine pancreas. In this study, we evaluated the methods for making a continuous density gradient and loading tissue. One method involved loading digested tissue on top of a continuous density gradient (top loading). The other method involved mixing digested tissue with low-density solution and then making a continuous gradient (mixed loading). There were no significant differences between the 2 purification methods in terms of the islet yield, rate of viability or purity, score, or in the stimulation index after purification. Furthermore, there were no marked differences in the attainability or suitability of posttransplantation normoglycemia. Our study shows the equivalency of these 2 methods of islet purification.
http://purl.org/coar/resource_type/c_6501
SAGE Publishing
2018-05
VoR
2155-1790
Cell Medicine
10
eng
https://doi.org/10.1177/2155179017733090
open access
© The Author(s) 2018
Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (http://www.creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).