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A leaf of citrus was cut into 5x5 mm pieces, crushed and softened with hot water for 10 min. A 100 \u03bcl sample of sap containing the infectious agent placed in a micro plate with 40 \u03bcl 5 mM iodine solution turned purple as a result of the iodo-starch reaction. Leaf samples from trees with deficiency symptoms gave a reaction ranging from light blue to blue, and healthy tree samples became yellow or brown, a result that distinguished diseased trees from healthy ones with the naked eye. Moreover, diseased samples had 20 times the optical absorbance as healthy ones at 540 nm. A comparison of PCR results with the iodo-starch assay resulted in 75% agreement for leaf samples, and 95% for trees.", "subitem_description_type": "Other"}, {"subitem_description": "\u672c\u7814\u7a76\u3067\u306f\u4f4e\u30b3\u30b9\u30c8\u3067\uff0c\u8fc5\u901f\u306b\u591a\u91cf\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u691c\u5b9a\u3067\u304d\u308b\u30ab\u30f3\u30ad\u30c4\u30b0\u30ea\u30fc\u30cb\u30f3\u30b0\u75c5\u306e\u691c\u5b9a\u6cd5\u306e\u958b\u767a\u3092\u884c\u3063\u305f\u3002\u307e\u305a\uff0c\u30ab\u30f3\u30ad\u30c4\u306e\u8449\u3092\u7d045\u00d75mm\u5927\u306b\u3061\u304e\u3063\u3066\u7834\u7815\u3057\uff0c\u3053\u308c\u309210\u5206\u9593\u71b1\u6e6f\u3067\u7cca\u5316\u3057\u305f\u5f8c\u3001\u305d\u306e\u4e0a\u6e05\u3092100\u03bcl\u53d6\u3063\u3066\u30de\u30a4\u30af\u30ed\u30d7\u30ec\u30fc\u30c8\u306b\u5165\u308c\uff0c\u3053\u308c\u306b5mM\u306e\u30e8\u30a6\u7d20\u6eb6\u6db2\u309240\u03bcl\u6dfb\u52a0\u3059\u308b\u3068\uff0c\u30ab\u30f3\u30ad\u30c4\u30b0\u30ea\u30fc\u30cb\u30f3\u30b0\u75c5\u306b\u7f79\u75c5\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u306f\u30e8\u30a6\u7d20\u30c7\u30f3\u30d7\u30f3\u53cd\u5fdc\u306b\u3088\u308a\u7d2b\u306b\uff0c\u8981\u7d20\u6b20\u4e4f\u75c7\u72b6\u306f\u6de1\u3044\u9752\uff5e\u9752\u8272\u306b\uff0c\u5065\u5168\u30b5\u30f3\u30d7\u30eb\u306f\uff0c\u6de1\u3044\u9752\uff0c\u9ec4\u307e\u305f\u306f\u8336\u8272\u306b\u767a\u8272\u3059\u308b\u305f\u3081\uff0c\u8089\u773c\u3067\u306e\u5224\u5225\u304c\u53ef\u80fd\u3067\u3042\u3063\u305f\u3002\u307e\u305f\u6ce2\u9577540nm\u3067\u5438\u5149\u5024\u3092\u6e2c\u5b9a\u3057\u305f\u7d50\u679c\uff0c\u7f79\u75c5\u30b5\u30f3\u30d7\u30eb\u306f\u5065\u5168\u30b5\u30f3\u30d7\u30eb\u306e20\u500d\u4ee5\u4e0a\u306e\u5438\u5149\u5024\u3092\u793a\u3057\u305f\u3002PCR\u3068\u30e8\u30a6\u7d20\u30fb\u30c7\u30f3\u30d7\u30f3\u53cd\u5fdc\u306b\u3088\u308b\u691c\u5b9a\u7d50\u679c\u3092\u6bd4\u8f03\u3057\u305f\u7d50\u679c\u3067\u306f\uff0c\u8449\u5f53\u305f\u308a\u3067\u306f75\uff05\uff0c\u6a39\u5f53\u305f\u308a\u3067\u306f95\uff05\u306e\u4e00\u81f4\u7387\u3092\u793a\u3057\u305f\u3002", "subitem_description_type": "Other"}, {"subitem_description": "\u7d00\u8981\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/607"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. 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