{"_buckets": {"deposit": "17db6da1-7376-49e3-963a-a81786eff4cb"}, "_deposit": {"id": "2001219", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2001219"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02001219", "sets": ["1642837924182", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "Detection of Citrus Huanglongbing using an Iodo-starch Reaction", "subitem_1551255648112": "en"}, {"subitem_1551255647225": "\u30e8\u30a6\u7d20\u30c7\u30f3\u30d7\u30f3\u53cd\u5fdc\u3092\u5229\u7528\u3057\u305f\u30ab\u30f3\u30ad\u30c4\u30b0\u30ea\u30fc\u30cb\u30f3\u30b0\u75c5\u306e\u691c\u51fa", "subitem_1551255648112": "ja"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Taba, Satoshi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nasu, Kanami", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Takaesu, Kazuko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Ooshiro, Atsushi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Moromizato, Zen-ichi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "\u7530\u5834, \u8061", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u90a3\u9808, \u594f\u7f8e", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u9ad8\u6c5f\u6d32, \u548c\u5b50", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u5927\u57ce, \u7be4", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u8af8\u898b\u91cc, \u5584\u4e00", "creatorNameLang": "ja"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186609386": {"attribute_name": "Subject", "attribute_value_mlt": [{"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "assay"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "greening"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "iodine"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "naked eye"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "starch"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u30c7\u30f3\u30d7\u30f3"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u30b0\u30ea\u30fc\u30cb\u30f3\u30b0"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u691c\u5b9a"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u8089\u773c"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u30e8\u30a6\u7d20"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "A rapid, low cost method for the detection of physiological changes associated with the causal agent of huanglongbing (greening) in citrus was developed. A leaf of citrus was cut into 5x5 mm pieces, crushed and softened with hot water for 10 min. A 100 \u03bcl sample of sap containing the infectious agent placed in a micro plate with 40 \u03bcl 5 mM iodine solution turned purple as a result of the iodo-starch reaction. Leaf samples from trees with deficiency symptoms gave a reaction ranging from light blue to blue, and healthy tree samples became yellow or brown, a result that distinguished diseased trees from healthy ones with the naked eye. Moreover, diseased samples had 20 times the optical absorbance as healthy ones at 540 nm. A comparison of PCR results with the iodo-starch assay resulted in 75% agreement for leaf samples, and 95% for trees.", "subitem_description_type": "Other"}, {"subitem_description": "\u672c\u7814\u7a76\u3067\u306f\u4f4e\u30b3\u30b9\u30c8\u3067\uff0c\u8fc5\u901f\u306b\u591a\u91cf\u306e\u30b5\u30f3\u30d7\u30eb\u3092\u691c\u5b9a\u3067\u304d\u308b\u30ab\u30f3\u30ad\u30c4\u30b0\u30ea\u30fc\u30cb\u30f3\u30b0\u75c5\u306e\u691c\u5b9a\u6cd5\u306e\u958b\u767a\u3092\u884c\u3063\u305f\u3002\u307e\u305a\uff0c\u30ab\u30f3\u30ad\u30c4\u306e\u8449\u3092\u7d045\u00d75mm\u5927\u306b\u3061\u304e\u3063\u3066\u7834\u7815\u3057\uff0c\u3053\u308c\u309210\u5206\u9593\u71b1\u6e6f\u3067\u7cca\u5316\u3057\u305f\u5f8c\u3001\u305d\u306e\u4e0a\u6e05\u3092100\u03bcl\u53d6\u3063\u3066\u30de\u30a4\u30af\u30ed\u30d7\u30ec\u30fc\u30c8\u306b\u5165\u308c\uff0c\u3053\u308c\u306b5mM\u306e\u30e8\u30a6\u7d20\u6eb6\u6db2\u309240\u03bcl\u6dfb\u52a0\u3059\u308b\u3068\uff0c\u30ab\u30f3\u30ad\u30c4\u30b0\u30ea\u30fc\u30cb\u30f3\u30b0\u75c5\u306b\u7f79\u75c5\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u306f\u30e8\u30a6\u7d20\u30c7\u30f3\u30d7\u30f3\u53cd\u5fdc\u306b\u3088\u308a\u7d2b\u306b\uff0c\u8981\u7d20\u6b20\u4e4f\u75c7\u72b6\u306f\u6de1\u3044\u9752\uff5e\u9752\u8272\u306b\uff0c\u5065\u5168\u30b5\u30f3\u30d7\u30eb\u306f\uff0c\u6de1\u3044\u9752\uff0c\u9ec4\u307e\u305f\u306f\u8336\u8272\u306b\u767a\u8272\u3059\u308b\u305f\u3081\uff0c\u8089\u773c\u3067\u306e\u5224\u5225\u304c\u53ef\u80fd\u3067\u3042\u3063\u305f\u3002\u307e\u305f\u6ce2\u9577540nm\u3067\u5438\u5149\u5024\u3092\u6e2c\u5b9a\u3057\u305f\u7d50\u679c\uff0c\u7f79\u75c5\u30b5\u30f3\u30d7\u30eb\u306f\u5065\u5168\u30b5\u30f3\u30d7\u30eb\u306e20\u500d\u4ee5\u4e0a\u306e\u5438\u5149\u5024\u3092\u793a\u3057\u305f\u3002PCR\u3068\u30e8\u30a6\u7d20\u30fb\u30c7\u30f3\u30d7\u30f3\u53cd\u5fdc\u306b\u3088\u308b\u691c\u5b9a\u7d50\u679c\u3092\u6bd4\u8f03\u3057\u305f\u7d50\u679c\u3067\u306f\uff0c\u8449\u5f53\u305f\u308a\u3067\u306f75\uff05\uff0c\u6a39\u5f53\u305f\u308a\u3067\u306f95\uff05\u306e\u4e00\u81f4\u7387\u3092\u793a\u3057\u305f\u3002", "subitem_description_type": "Other"}, {"subitem_description": "\u7d00\u8981\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/607"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2006-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "53", "bibliographicPageEnd": "24", "bibliographicPageStart": "19"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "NA", "subitem_1600292170262": "http://purl.org/coar/version/c_be7fb7dd8ff6fe43"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "04_taba.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2001219/files/04_taba.pdf"}, "version_id": "b33255da-06f7-49e1-8753-44ca037ac6fd"}]}, "item_title": "Detection of Citrus Huanglongbing using an Iodo-starch Reaction", "item_type_id": "15", "owner": "1", "path": ["1642837924182", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/607", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2007-06-25"}, "publish_date": "2007-06-25", "publish_status": "0", "recid": "2001219", "relation": {}, "relation_version_is_last": true, "title": ["Detection of Citrus Huanglongbing using an Iodo-starch Reaction"], "weko_shared_id": -1}
ヨウ素デンプン反応を利用したカンキツグリーニング病の検出
http://hdl.handle.net/20.500.12000/607
http://hdl.handle.net/20.500.12000/607