{"_buckets": {"deposit": "17db6da1-7376-49e3-963a-a81786eff4cb"}, "_deposit": {"id": "2001219", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2001219"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02001219", "sets": ["1642837924182", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "Detection of Citrus Huanglongbing using an Iodo-starch Reaction", "subitem_1551255648112": "en"}, {"subitem_1551255647225": "ヨウ素デンプン反応を利用したカンキツグリーニング病の検出", "subitem_1551255648112": "ja"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Taba, Satoshi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nasu, Kanami", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Takaesu, Kazuko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Ooshiro, Atsushi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Moromizato, Zen-ichi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "田場, 聡", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "那須, 奏美", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "高江洲, 和子", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "大城, 篤", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "諸見里, 善一", "creatorNameLang": "ja"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186609386": {"attribute_name": "Subject", "attribute_value_mlt": [{"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "assay"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "greening"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "iodine"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "naked eye"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "starch"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "デンプン"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "グリーニング"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "検定"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "肉眼"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "ヨウ素"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "A rapid, low cost method for the detection of physiological changes associated with the causal agent of huanglongbing (greening) in citrus was developed. A leaf of citrus was cut into 5x5 mm pieces, crushed and softened with hot water for 10 min. A 100 μl sample of sap containing the infectious agent placed in a micro plate with 40 μl 5 mM iodine solution turned purple as a result of the iodo-starch reaction. Leaf samples from trees with deficiency symptoms gave a reaction ranging from light blue to blue, and healthy tree samples became yellow or brown, a result that distinguished diseased trees from healthy ones with the naked eye. Moreover, diseased samples had 20 times the optical absorbance as healthy ones at 540 nm. A comparison of PCR results with the iodo-starch assay resulted in 75% agreement for leaf samples, and 95% for trees.", "subitem_description_type": "Other"}, {"subitem_description": "本研究では低コストで，迅速に多量のサンプルを検定できるカンキツグリーニング病の検定法の開発を行った。まず，カンキツの葉を約5×5mm大にちぎって破砕し，これを10分間熱湯で糊化した後、その上清を100μl取ってマイクロプレートに入れ，これに5mMのヨウ素溶液を40μl添加すると，カンキツグリーニング病に罹病したサンプルはヨウ素デンプン反応により紫に，要素欠乏症状は淡い青～青色に，健全サンプルは，淡い青，黄または茶色に発色するため，肉眼での判別が可能であった。また波長540nmで吸光値を測定した結果，罹病サンプルは健全サンプルの20倍以上の吸光値を示した。PCRとヨウ素・デンプン反応による検定結果を比較した結果では，葉当たりでは75％，樹当たりでは95％の一致率を示した。", "subitem_description_type": "Other"}, {"subitem_description": "紀要論文", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "琉球大学農学部"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/607"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "琉球大学農学部学術報告"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2006-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "53", "bibliographicPageEnd": "24", "bibliographicPageStart": "19"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "NA", "subitem_1600292170262": "http://purl.org/coar/version/c_be7fb7dd8ff6fe43"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "04_taba.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2001219/files/04_taba.pdf"}, "version_id": "b33255da-06f7-49e1-8753-44ca037ac6fd"}]}, "item_title": "Detection of Citrus Huanglongbing using an Iodo-starch Reaction", "item_type_id": "15", "owner": "1", "path": ["1642837924182", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/607", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2007-06-25"}, "publish_date": "2007-06-25", "publish_status": "0", "recid": "2001219", "relation": {}, "relation_version_is_last": true, "title": ["Detection of Citrus Huanglongbing using an Iodo-starch Reaction"], "weko_shared_id": -1}