@article{oai:u-ryukyu.repo.nii.ac.jp:02001219,
 author = {Taba, Satoshi and Nasu, Kanami and Takaesu, Kazuko and Ooshiro, Atsushi and Moromizato, Zen-ichi and 田場, 聡 and 那須, 奏美 and 高江洲, 和子 and 大城, 篤 and 諸見里, 善一},
 issue = {53},
 journal = {琉球大学農学部学術報告, The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus},
 month = {Dec},
 note = {A rapid, low cost method for the detection of physiological changes associated with the causal agent of huanglongbing (greening) in citrus was developed. A leaf of citrus was cut into 5x5 mm pieces, crushed and softened with hot water for 10 min. A 100 μl sample of sap containing the infectious agent placed in a micro plate with 40 μl 5 mM iodine solution turned purple as a result of the iodo-starch reaction. Leaf samples from trees with deficiency symptoms gave a reaction ranging from light blue to blue, and healthy tree samples became yellow or brown, a result that distinguished diseased trees from healthy ones with the naked eye. Moreover, diseased samples had 20 times the optical absorbance as healthy ones at 540 nm. A comparison of PCR results with the iodo-starch assay resulted in 75% agreement for leaf samples, and 95% for trees., 本研究では低コストで，迅速に多量のサンプルを検定できるカンキツグリーニング病の検定法の開発を行った。まず，カンキツの葉を約5×5mm大にちぎって破砕し，これを10分間熱湯で糊化した後、その上清を100μl取ってマイクロプレートに入れ，これに5mMのヨウ素溶液を40μl添加すると，カンキツグリーニング病に罹病したサンプルはヨウ素デンプン反応により紫に，要素欠乏症状は淡い青～青色に，健全サンプルは，淡い青，黄または茶色に発色するため，肉眼での判別が可能であった。また波長540nmで吸光値を測定した結果，罹病サンプルは健全サンプルの20倍以上の吸光値を示した。PCRとヨウ素・デンプン反応による検定結果を比較した結果では，葉当たりでは75％，樹当たりでは95％の一致率を示した。, 紀要論文},
 pages = {19--24},
 title = {Detection of Citrus Huanglongbing using an Iodo-starch Reaction},
 year = {2006}
}