{"created":"2022-01-25T01:40:51.455092+00:00","id":2001219,"links":{},"metadata":{"_buckets":{"deposit":"17db6da1-7376-49e3-963a-a81786eff4cb"},"_deposit":{"id":"2001219","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2001219"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02001219","sets":["1642837622505:1642837905044:1642837924182","1642838403551:1642838407312"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"Detection of Citrus Huanglongbing using an Iodo-starch Reaction","subitem_1551255648112":"en"},{"subitem_1551255647225":"ヨウ素デンプン反応を利用したカンキツグリーニング病の検出","subitem_1551255648112":"ja"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Taba, Satoshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nasu, Kanami","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takaesu, Kazuko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ooshiro, Atsushi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Moromizato, Zen-ichi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"田場, 聡","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"那須, 奏美","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"高江洲, 和子","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"大城, 篤","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"諸見里, 善一","creatorNameLang":"ja"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"assay"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"greening"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"iodine"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"naked eye"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"starch"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"デンプン"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"グリーニング"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"検定"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"肉眼"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"ヨウ素"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"A rapid, low cost method for the detection of physiological changes associated with the causal agent of huanglongbing (greening) in citrus was developed. A leaf of citrus was cut into 5x5 mm pieces, crushed and softened with hot water for 10 min. A 100 μl sample of sap containing the infectious agent placed in a micro plate with 40 μl 5 mM iodine solution turned purple as a result of the iodo-starch reaction. Leaf samples from trees with deficiency symptoms gave a reaction ranging from light blue to blue, and healthy tree samples became yellow or brown, a result that distinguished diseased trees from healthy ones with the naked eye. Moreover, diseased samples had 20 times the optical absorbance as healthy ones at 540 nm. A comparison of PCR results with the iodo-starch assay resulted in 75% agreement for leaf samples, and 95% for trees.","subitem_description_type":"Other"},{"subitem_description":"本研究では低コストで，迅速に多量のサンプルを検定できるカンキツグリーニング病の検定法の開発を行った。まず，カンキツの葉を約5×5mm大にちぎって破砕し，これを10分間熱湯で糊化した後、その上清を100μl取ってマイクロプレートに入れ，これに5mMのヨウ素溶液を40μl添加すると，カンキツグリーニング病に罹病したサンプルはヨウ素デンプン反応により紫に，要素欠乏症状は淡い青～青色に，健全サンプルは，淡い青，黄または茶色に発色するため，肉眼での判別が可能であった。また波長540nmで吸光値を測定した結果，罹病サンプルは健全サンプルの20倍以上の吸光値を示した。PCRとヨウ素・デンプン反応による検定結果を比較した結果では，葉当たりでは75％，樹当たりでは95％の一致率を示した。","subitem_description_type":"Other"},{"subitem_description":"紀要論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"琉球大学農学部"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/607"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0370-4246"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN00250548"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"琉球大学農学部学術報告"},{"subitem_1522650068558":"en","subitem_1522650091861":"The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-12","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"53","bibliographicPageEnd":"24","bibliographicPageStart":"19"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"NA","subitem_1600292170262":"http://purl.org/coar/version/c_be7fb7dd8ff6fe43"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"04_taba.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2001219/files/04_taba.pdf"},"version_id":"b33255da-06f7-49e1-8753-44ca037ac6fd"}]},"item_title":"Detection of Citrus Huanglongbing using an Iodo-starch Reaction","item_type_id":"15","owner":"1","path":["1642837924182","1642838407312"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2007-06-25"},"publish_date":"2007-06-25","publish_status":"0","recid":"2001219","relation_version_is_last":true,"title":["Detection of Citrus Huanglongbing using an Iodo-starch Reaction"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T00:44:14.513465+00:00"}