{"_buckets": {"deposit": "3c22e9af-04f0-43ad-b35b-e5ee7428fe4c"}, "_deposit": {"id": "2002620", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2002620"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02002620", "sets": ["1642837923205", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "\u6db2\u4f53\u57f9\u990a\u306b\u3088\u308b\u7d05\u9eb9\u83cc\u306e\u30ab\u30eb\u30dc\u30ad\u30b7\u30da\u30d7\u30c1\u30c0\u30fc\u30bc\u306e\u751f\u7523", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Production of Carboxypeptidase by Monascus purpureus in Submerged Culture", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "?, \u82b3", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u6a58, \u4fe1\u4e8c\u90ce", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u5b89\u7530, \u6b63\u662d", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Liu, Fang", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Tachibana, Shinjiro", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Yasuda, Masaaki", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186609386": {"attribute_name": "Subject", "attribute_value_mlt": [{"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u7d05\u9eb9\u83cc"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u30ab\u30eb\u30dc\u30ad\u30b7\u30da\u30d7\u30c6\u30c0\u30fc\u30bc"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u6db2\u4f53\u57f9\u990a"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u3068\u3046\u3075\u3088\u3046"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "Monascus purpureus"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "carboxypeptidase"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "submerged culture"}, {"subitem_1522299896455": "en", "subitem_1522300014469": "Other", "subitem_1523261968819": "tofuyo"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "\u30ab\u30eb\u30dc\u30ad\u30b7\u30da\u30d7\u30c2\u30c0\u30fc\u30bc\u306f,\u30bf\u30f3\u30d1\u30af\u8cea\u3084\u30da\u30d7\u30c1\u30c9\u306e\u30ab\u30eb\u30dc\u30ad\u30b7\u30eb\u672b\u7aef\u304b\u3089\u30a2\u30df\u30ce\u9178\u3092\u9806\u6b21\u904a\u96e2\u3059\u308b\u9175\u7d20\u3067\u3042\u308b.\u7d05\u9eb9\u83cc(Monascus\u5c5e\u30ab\u30d3)\u306f,\u4e2d\u56fd\u3067\u306f\u7d05\u8150\u4e73\u3084\u7d05\u9152,\u6c96\u7e04\u3067\u306f\u3068\u3046\u3075\u3088\u3046\u306e\u88fd\u9020\u306b\u53e4\u304f\u304b\u3089\u4f7f\u7528\u3055\u308c\u3066\u304d\u305f\u98df\u54c1\u5fae\u751f\u7269\u3067\u3042\u308b.\u6211\u3005\u306f,\u3053\u308c\u307e\u3067\u306b\u3068\u3046\u3075\u3088\u3046\u306e\u98df\u54c1\u79d1\u5b66\u7684\u7279\u6027\u3092\u660e\u3089\u304b\u306b\u3057\u3066\u304a\u308a,\u672c\u767a\u9175\u98df\u54c1\u306e\u30a2\u30df\u30ce\u9178\u7cfb\u5448\u6627\u6210\u5206\u306e\u751f\u6210\u306b\u30ab\u30eb\u30dc\u30ad\u30b7\u30da\u30d7\u30c6\u30c0\u30fc\u30bc\u304c\u91cd\u8981\u306a\u5f79\u5272\u3092\u6f14\u3058\u3066\u3044\u308b\u3068\u8003\u3048\u3089\u308c\u308b.\u672c\u7814\u7a76\u3067\u306f,\u3068\u3046\u3075\u3088\u3046\u88fd\u9020\u304a\u3088\u3073\u5448\u5473\u5f62\u6210\u306b\u91cd\u8981\u3067\u3042\u308b\u3068\u8003\u3048\u3089\u308c\u308b\u7d05\u9eb9\u83cc\u306e\u751f\u7523\u3059\u308b\u30ab\u30eb\u30dc\u30ad\u30b7\u30da\u30d7\u30c1\u30c0\u30fc\u30bc\u306b\u7740\u76ee\u3057,\u672c\u9175\u7d20\u6d3b\u6027\u306e\u9ad8\u3044\u512a\u826f\u83cc\u682a\u306e\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0,\u672c\u9175\u7d20\u751f\u7523\u306e\u57f9\u5730\u306e\u7d44\u6210\u3068\u57f9\u990a\u6761\u4ef6\u306e\u78ba\u7acb\u3092\u76ee\u7684\u3068\u3057\u305f.\u307e\u305a,\u7d05\u9eb9\u83cc28\u83cc\u682a\u3092\u7528\u3044\u3066,\u30ab\u30eb\u30dc\u30ad\u30b7\u30da\u30d7\u30c6\u30c0\u30fc\u30bc\u9ad8\u751f\u7523\u83cc\u682a\u306e1\u6b21\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3092\u884c\u3044,\u672c\u9175\u7d20\u6d3b\u6027\u304c\u9ad8\u30446\u83cc\u682a\u3092\u9078\u629e\u3057\u305f.\u6b21\u306b,2\u6b21\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3092\u884c\u3044,\u672c\u9175\u7d20\u6d3b\u6027\u53ca\u3073\u83cc\u4f53\u751f\u80b2\u306e\u6700\u3082\u826f\u597d\u306aMonascus purp\u3072reus IFO4478\u3092\u30ab\u30eb\u30dc\u30ad\u30b7\u30da\u30d7\u30c1\u30c0\u30fc\u30bc\u9ad8\u7523\u751f\u512a\u826f\u83cc\u682a\u3068\u3057\u3066\u9078\u629e\u3057\u305f.\u7d9a\u3044\u3066,\u672c\u83cc\u682a\u3092\u7528\u3044\u3066\u30ab\u30eb\u30dc\u30ad\u30b7\u30da\u30d7\u30c6\u30c0\u30fc\u30bc\u306e\u6700\u9069\u751f\u7523\u6761\u4ef6\u3092\u8abf\u3079\u305f.\u305d\u306e\u7d50\u679c,\u672c\u9175\u7d20\u751f\u7523\u306e\u6700\u9069\u57f9\u990a\u7d44\u6210\u3068\u57f9\u990a\u6761\u4ef6\u306f\u6b21\u306e\u3088\u3046\u306b\u8a2d\u5b9a\u3057\u305f.(1)\u57f9\u5730\u7d44\u6210:\u30b0\u30eb\u30b3\u30fc\u30b91%(w/v),\u5206\u96e2\u5927\u8c46\u30bf\u30f3\u30d1\u30af\u8cea2%(w/v),MgSO_4\u30fb7H_2O 0.5%(w/v),KH_2PO_4 0.5%(w/v),\u30df\u30cd\u30e9\u30eb\u6eb6\u6db2A,B and C\u54040.1%(v/v)\u53ca\u3073\u57f9\u5730\u306e\u521dpH\u304c6.0, (2)\u57f9\u990a\u6761\u4ef6:28\u2103,150rum,6\u65e5\u9593\u632f\u3068\u3046\u57f9\u990a.", "subitem_description_type": "Other"}, {"subitem_description": "In this study, the screening test on twenty eight strains of Monascus for the production of carboxypeptidasse (CPase) in submerged culture was performed, Monascus purpureus IFO 4478 was indicated to be the best producer of CPase. The culture medium compositions and cultivation conditions for the production of CPase by this strain during submerged culture in shake flasks were optimized. Subsequently the process was scaled up to 5 L jar fermentor. The results of carbon source test showed that glucose was the best inducer of CPase among the eleven kinds of carbon sources tested with the addition of 1% (w/v). For the nitrogen source test, soybean protein isolate (SPI) was the best inducer of CPase among the seventeen kinds of nitrogen sources tested with the addition of 2% (w/v). Moreover, the mineral salts solutions with the addition of each 0.1% (v/v) could induce the production of CPase, and when the initial pH of the culture medium was 6.0, the maximal CPase activity was obtained. In addition, the effects of shaking speed, incubation temperature and incubation time on CPase production were also investigated. The results indicated that when M. purpureus IFO 4478 was cultivated in a reciprocal shake incubator with a shaking speed 150 rpm and at 28\u00b0C, the maximal CPase activity was obtained after 6 days. Therefore, the optimal culture medium for the CPase production was: glucose 1.0% (w/v), SPI 2.0% (w/v), MgSO4 \u30fb 7H2O 0. 5% (w/v), KH2PO4 0.5 % (w/v), mineral salt solutions 0.1% (v/v) (pH 6.0), and the optimal cultivation condition was: at 150 rpm and 28\u00b0C for 6 days.", "subitem_description_type": "Other"}, {"subitem_description": "\u7d00\u8981\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "eng"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/3577"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "51", "bibliographicPageEnd": "138", "bibliographicPageStart": "131"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00004292645.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2002620/files/KJ00004292645.pdf"}, "version_id": "d40776b2-8e52-4292-b3a0-324ed54c3ee0"}]}, "item_title": "\u6db2\u4f53\u57f9\u990a\u306b\u3088\u308b\u7d05\u9eb9\u83cc\u306e\u30ab\u30eb\u30dc\u30ad\u30b7\u30da\u30d7\u30c1\u30c0\u30fc\u30bc\u306e\u751f\u7523", "item_type_id": "15", "owner": "1", "path": ["1642837923205", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/3577", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-13"}, "publish_date": "2008-02-13", "publish_status": "0", "recid": "2002620", "relation": {}, "relation_version_is_last": true, "title": ["\u6db2\u4f53\u57f9\u990a\u306b\u3088\u308b\u7d05\u9eb9\u83cc\u306e\u30ab\u30eb\u30dc\u30ad\u30b7\u30da\u30d7\u30c1\u30c0\u30fc\u30bc\u306e\u751f\u7523"], "weko_shared_id": -1}