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  1. 紀要論文
  2. 琉球大学農学部学術報告
  3. 51号
  1. 部局別インデックス
  2. 農学部

液体培養による紅麹菌のカルボキシペプチダーゼの生産

http://hdl.handle.net/20.500.12000/3577
http://hdl.handle.net/20.500.12000/3577
5716a6c0-9e2d-4d5b-9f03-4921524ecd0d
名前 / ファイル ライセンス アクション
KJ00004292645.pdf KJ00004292645.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2008-02-13
タイトル
タイトル 液体培養による紅麹菌のカルボキシペプチダーゼの生産
言語 ja
タイトル
タイトル Production of Carboxypeptidase by Monascus purpureus in Submerged Culture
言語 en
作成者 ?, 芳

× ?, 芳

ja ?, 芳

Search repository
橘, 信二郎

× 橘, 信二郎

ja 橘, 信二郎

Search repository
安田, 正昭

× 安田, 正昭

ja 安田, 正昭

Search repository
Liu, Fang

× Liu, Fang

en Liu, Fang

Search repository
Tachibana, Shinjiro

× Tachibana, Shinjiro

en Tachibana, Shinjiro

Search repository
Yasuda, Masaaki

× Yasuda, Masaaki

en Yasuda, Masaaki

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
主題
言語 ja
主題Scheme Other
主題 紅麹菌
主題
言語 ja
主題Scheme Other
主題 カルボキシペプテダーゼ
主題
言語 ja
主題Scheme Other
主題 液体培養
主題
言語 ja
主題Scheme Other
主題 とうふよう
主題
言語 en
主題Scheme Other
主題 Monascus purpureus
主題
言語 en
主題Scheme Other
主題 carboxypeptidase
主題
言語 en
主題Scheme Other
主題 submerged culture
主題
言語 en
主題Scheme Other
主題 tofuyo
内容記述
内容記述タイプ Other
内容記述 カルボキシペプヂダーゼは,タンパク質やペプチドのカルボキシル末端からアミノ酸を順次遊離する酵素である.紅麹菌(Monascus属カビ)は,中国では紅腐乳や紅酒,沖縄ではとうふようの製造に古くから使用されてきた食品微生物である.我々は,これまでにとうふようの食品科学的特性を明らかにしており,本発酵食品のアミノ酸系呈昧成分の生成にカルボキシペプテダーゼが重要な役割を演じていると考えられる.本研究では,とうふよう製造および呈味形成に重要であると考えられる紅麹菌の生産するカルボキシペプチダーゼに着目し,本酵素活性の高い優良菌株のスクリーニング,本酵素生産の培地の組成と培養条件の確立を目的とした.まず,紅麹菌28菌株を用いて,カルボキシペプテダーゼ高生産菌株の1次スクリーニングを行い,本酵素活性が高い6菌株を選択した.次に,2次スクリーニングを行い,本酵素活性及び菌体生育の最も良好なMonascus purpひreus IFO4478をカルボキシペプチダーゼ高産生優良菌株として選択した.続いて,本菌株を用いてカルボキシペプテダーゼの最適生産条件を調べた.その結果,本酵素生産の最適培養組成と培養条件は次のように設定した.(1)培地組成:グルコース1%(w/v),分離大豆タンパク質2%(w/v),MgSO_4・7H_2O 0.5%(w/v),KH_2PO_4 0.5%(w/v),ミネラル溶液A,B and C各0.1%(v/v)及び培地の初pHが6.0, (2)培養条件:28℃,150rum,6日間振とう培養.
内容記述
内容記述タイプ Other
内容記述 In this study, the screening test on twenty eight strains of Monascus for the production of carboxypeptidasse (CPase) in submerged culture was performed, Monascus purpureus IFO 4478 was indicated to be the best producer of CPase. The culture medium compositions and cultivation conditions for the production of CPase by this strain during submerged culture in shake flasks were optimized. Subsequently the process was scaled up to 5 L jar fermentor. The results of carbon source test showed that glucose was the best inducer of CPase among the eleven kinds of carbon sources tested with the addition of 1% (w/v). For the nitrogen source test, soybean protein isolate (SPI) was the best inducer of CPase among the seventeen kinds of nitrogen sources tested with the addition of 2% (w/v). Moreover, the mineral salts solutions with the addition of each 0.1% (v/v) could induce the production of CPase, and when the initial pH of the culture medium was 6.0, the maximal CPase activity was obtained. In addition, the effects of shaking speed, incubation temperature and incubation time on CPase production were also investigated. The results indicated that when M. purpureus IFO 4478 was cultivated in a reciprocal shake incubator with a shaking speed 150 rpm and at 28°C, the maximal CPase activity was obtained after 6 days. Therefore, the optimal culture medium for the CPase production was: glucose 1.0% (w/v), SPI 2.0% (w/v), MgSO4 ・ 7H2O 0. 5% (w/v), KH2PO4 0.5 % (w/v), mineral salt solutions 0.1% (v/v) (pH 6.0), and the optimal cultivation condition was: at 150 rpm and 28°C for 6 days.
内容記述
内容記述タイプ Other
内容記述 紀要論文
出版者
言語 ja
出版者 琉球大学農学部
言語
言語 eng
資源タイプ
資源タイプ departmental bulletin paper
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
識別子
識別子 http://hdl.handle.net/20.500.12000/3577
識別子タイプ HDL
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0370-4246
収録物識別子
収録物識別子タイプ NCID
収録物識別子 AN00250548
収録物名
言語 ja
収録物名 琉球大学農学部学術報告
収録物名
言語 en
収録物名 The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus
書誌情報
号 51, p. 131-138
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