@article{oai:u-ryukyu.repo.nii.ac.jp:02002772, author = {日越, 博信 and 具志堅, 宏 and 平川, 守彦 and Higoshi, Hironobu and Gushiken, Hiroshi and Hirakawa, Morihiko}, issue = {42}, journal = {琉球大学農学部学術報告, The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus}, month = {Dec}, note = {沖縄県内の3農場で肥育中の肉用牛, 64頭(和種25頭, 外産牛24頭, 乳F_1 15頭)の糞便から分離した大腸菌, 合計610株について薬剤感受性試験を行った。供試した7薬剤のいずれかに耐性であった菌株は, 136株(22.3%)であった。ウシの品種等別にみた耐性菌検出率は, 和種30.6%, 外産牛16.7%, 乳F_1 17.7%であり, 和種が高かった。薬剤別の耐性菌検出率は, 全体ではCTC耐性12.8%, SA耐性10.2%などが高く, 他の薬剤耐性はいずれも5%未満であった。耐性型の種類は単剤型から4剤型まで14種類認められたが, 外産牛では11種類, 和種では9種類, 乳F_1では6種類であった。しかし, 外産牛と乳F_1では単剤型が71∿62%を占めたのに対し, 和種では2剤以上に耐性の多剤耐性型が過半数を占めた。耐性菌における伝達性Rプラスミド保有菌の検出率は, 12.7%であった。ウシの品種等別では, 外産牛が20%, 乳F_1が11.5%, 和種が10%であった。またこれらの伝達耐性型はすべて単剤型であった。, Escherichia coli was isolated from 64 head of the beef cattle fattened in 3 farms in Okinawa Prefecture [25 Japanese Blacks, 24 imported steers fattened in Okinawa (imported cattle) and 15 offsprings from Holstein and Japanese Black (F_1 hybrid)]. A total of 610 strains of E. coli (235 from Japanese Blacks, 228 from imported cattle and 147 from F_1 hybrid) were isolated and antibiotic sensitivity tests to ABPC, CP, CTC, KM, SM, NA and SA were performed. Of the 610 isolates, 136 (22.3%) were resistant to one of the antibiotics tested. The antibiotic-resistant E. coli isolation rate was 30.6% in Japanese Blacks, 16.7% in imported cattle and 17.7% in F_1 hybrid. The overall antibiotic resistant rates were 12.8% and 10.2% for CTC and SA, respectively, and less than 5% for the other antibiotics. Fourteen antibiotic resistance patterns, ranging from single-agent to 4-agent resistance were detected : eleven patterns in imported cattle, 9 patterns in Japanese Blacks and 6 patterns in F_1 hybrid. While 62 to 71% of the strains isolated from imported cattle and F_1 hybrid were resistant to a single agent, over 50% of the strains from Japanese Blacks were resistant to 2 or more antibiotics. Transmissible R plasmid were examined in the antibiotic resistant E. coli strains. Of the 126 strains examined, 16 (12.7%) possessed transmissible R plasmids. They were found in 20% of the resistant strains from imported cattle, 11.5% of those from F_1 hybrid and 10% of those from Japanese Blacks. The type of transmissible resistance was all one-agent type., 紀要論文}, pages = {33--38}, title = {肉用牛糞便由来大腸菌の薬剤耐性(生物生産学科)}, year = {1995} }