@article{oai:u-ryukyu.repo.nii.ac.jp:02002817, author = {日越, 博信 and 内田, 雅美 and 上原, 亮 and Higoshi, Hironobu and Uchida, Masami and Uehara, Makoto}, issue = {40}, journal = {琉球大学農学部学術報告, The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus}, month = {Dec}, note = {食鳥肉における薬剤耐性大腸菌の汚染状況を知る目的で,食鳥処理場の工程別に器具類,食鳥肉及び食鳥肉の解体器具類から大腸菌を分離し,ABPC, CTC, SM, CP, KM, NA, SAの7薬剤に対する感受性試験を行った。処理器具類由来大腸菌では,80.7%(271/336株)が耐性であった。食鳥肉由来では56.7%(76/134株)が,また解体器具類由来では41.5%(17/41株)が,それぞれ耐性であった。薬剤別の耐性菌検出率は,全体ではCTCが65.0%で最も高く,以下SM, SA, KMの順であった。また処理器具類と食鳥肉由来における耐性菌の検出順位は上記と同じであったが,解体器具類由来ではSA耐性が最も多かった。薬剤耐性型は,単剤耐性型から6剤耐性型まで23種類認められたが,工程が進むに伴ってその種類は減少した。また,耐性菌の84.7%が2剤以上に耐性の多剤耐性であったが,処理器具類由来の77%が3剤耐性型以上であったのに対し,食鳥肉及び解体器具類由来では64∿74%が逆に2剤耐性型以下であった。耐性菌におけるRプラスミド保有菌は,30株(9.7%)であり,少なかった。, To assess the contamination of poultry meat by drug resistant E. coli, the organism was isolated from machines and equipment in the processing plant, from poultry carcasses, and from dresing machines and equipment of the carcasses in each step of poultry meat processing. These organisms were tested for sensitivity to ABPC, CTC, SM, CP, KM, NA, and SA. In the E. coli found on machines and equipment in the processing plant such as the basket for transporting live animals, shackle, picker, abdominal spreader, and eviscerator, durg resistance was found in 271 of 336 strains (80.7%). Samples were taken from the carcasses after plucking, after evisceration, after preliminary cooling, and after final cooling. Drug resistance was seen in 56.7% (76 of 134) of these strains of E. coli. Samples from the dresing machines and equipment of the carcasses such as cutting table, knife, conveyor, and packing box revealed drug resistance in 41.5% (17 of 41) of the strains of E. coli detected. Resistance to CTC was the most common (65.0%) followed by resistance to SM, SA, and KM, in this order, among strains from the carcasses and from the machines and equipment in the procesing plant. Strains resistant to SA, however, were the most frequent finding from the dresing machines and equipment of the carcasses. Twenty three different patterns of resistance were detected. These ranged from resistance to a single drug up to resistance to six drugs. As the stage of poultry meat processing proceeded, however, the variety of patterns decreased. In 84.7% of the resistant strains, multiple drug resistance to more than two drugs was noted. While 77% of strains from the machines and equipment in the processing plant were resistant to more than three drugs, 64-74% of those from the carcasses and the dresing machines and equipment of the carcasses were resistant to less than two drugs. Attempts were made to detect R plasmid in the resistant strains. It was detected in only 30 strains (9.7%), a rather low frequecy., 紀要論文}, pages = {21--28}, title = {食鳥処理場の器具類及び食鳥肉由来大腸菌の薬剤耐性(生物生産学科)}, year = {1993} }