WEKO3
アイテム
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甘蔗茎の NPP- ホスファターゼの性質(農芸化学科)
http://hdl.handle.net/20.500.12000/3963
http://hdl.handle.net/20.500.12000/39637718b9ce-1660-4e09-b724-17d3ff58dc30
| 名前 / ファイル | ライセンス | アクション | |
|---|---|---|---|
KJ00000162203.pdf
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| Item type | デフォルトアイテムタイプ（フル）(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2008-02-14 | |||||||||
| タイトル | ||||||||||
| タイトル | 甘蔗茎の NPP- ホスファターゼの性質(農芸化学科) | |||||||||
| 言語 | ja | |||||||||
| 作成者 |
仲宗根, 洋子
× 仲宗根, 洋子
× Nakasone, Yoko
|
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| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 登熟甘蔗茎には, パラニトロフェニルリン酸を加水分解する酸性ホスファターゼが存在することを確認した。蔗汁より, 硫安塩析したのちに酸処理した酵素タンパク画分を, ホスファターゼ酵素標品として用いその性質をしらべた。本酵素は至適pHを5.5にもつ酸性ホスファターゼであった。他の植物起源の酸性ホスファターゼとことなって, 本酵素は1mMのEDTAやカルシウムイオンによりほとんど活性を失なった。本酵素は1mMの水銀イオンおよびフッ素イオンの阻害をうけたが, 銅イオンによっては全く阻害されなかった。また, 至適反応温度は50℃にあって, 10分間保持の40℃までは安定であった。 | |||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | The existence of an acid phosphatase in the mature cane stalks of NCO 376 variety, cultivated in Okinawa, was confirmed. The acid phosphatase hydrolyzing p-nitrophenyl phosphoric acid (NPP), from the extract of cane stalks, was partially purified by ammonium sulfate fractionation and acid treatment. This enzyme had an optimum pH of 5.5 and almost no activity at alkaline areas. It had Km value for NPP of 0.77mM and an optimum temperature of 50℃ and was stable at temperatures lower than 40℃. Magnesium, cupric and manganese ions in 1.16mM showed no effect on the enzyme activity. However, the inhibition of the activity by calcium and mercuric ions and EDTA in 1.16mM was remarkable, but that by fluoride ion in 1.16mM was moderate. EDTA and mercuric ion in 0.11mM, on the other hand, did not inactivate the enzyme. The modes of the inhibition by EDTA and calcium ion distinguish the enzyme studied in this work from the acid phosphatases obtained from several other plant species. | |||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 紀要論文 | |||||||||
| 出版者 | ||||||||||
| 言語 | ja | |||||||||
| 出版者 | 琉球大学農学部 | |||||||||
| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ | departmental bulletin paper | |||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 識別子 | ||||||||||
| 識別子 | http://hdl.handle.net/20.500.12000/3963 | |||||||||
| 識別子タイプ | HDL | |||||||||
| 収録物識別子 | ||||||||||
| 収録物識別子タイプ | ISSN | |||||||||
| 収録物識別子 | 0370-4246 | |||||||||
| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | AN00250548 | |||||||||
| 収録物名 | ||||||||||
| 言語 | ja | |||||||||
| 収録物名 | 琉球大学農学部学術報告 | |||||||||
| 書誌情報 |
号 31, p. 51-55 |
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