{"created":"2022-01-27T02:45:07.108776+00:00","id":2002992,"links":{},"metadata":{"_buckets":{"deposit":"1e9e4e7d-0457-4317-a560-8a302dda9be4"},"_deposit":{"id":"2002992","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2002992"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02002992","sets":["1642837622505:1642837905044:1642837914150","1642838403551:1642838407312"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"\u7518\u8517\u830e\u306e NPP- \u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u306e\u6027\u8cea(\u8fb2\u82b8\u5316\u5b66\u79d1)","subitem_1551255648112":"ja"},{"subitem_1551255647225":"Properties of NPP-phosphatase from cane stalks (Department of Agricultural Chemistry)","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"\u4ef2\u5b97\u6839, \u6d0b\u5b50","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Nakasone, Yoko","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"\u767b\u719f\u7518\u8517\u830e\u306b\u306f, \u30d1\u30e9\u30cb\u30c8\u30ed\u30d5\u30a7\u30cb\u30eb\u30ea\u30f3\u9178\u3092\u52a0\u6c34\u5206\u89e3\u3059\u308b\u9178\u6027\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u304c\u5b58\u5728\u3059\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u305f\u3002\u8517\u6c41\u3088\u308a, \u786b\u5b89\u5869\u6790\u3057\u305f\u306e\u3061\u306b\u9178\u51e6\u7406\u3057\u305f\u9175\u7d20\u30bf\u30f3\u30d1\u30af\u753b\u5206\u3092, \u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u9175\u7d20\u6a19\u54c1\u3068\u3057\u3066\u7528\u3044\u305d\u306e\u6027\u8cea\u3092\u3057\u3089\u3079\u305f\u3002\u672c\u9175\u7d20\u306f\u81f3\u9069pH\u30925.5\u306b\u3082\u3064\u9178\u6027\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u3067\u3042\u3063\u305f\u3002\u4ed6\u306e\u690d\u7269\u8d77\u6e90\u306e\u9178\u6027\u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u3068\u3053\u3068\u306a\u3063\u3066, \u672c\u9175\u7d20\u306f1mM\u306eEDTA\u3084\u30ab\u30eb\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3\u306b\u3088\u308a\u307b\u3068\u3093\u3069\u6d3b\u6027\u3092\u5931\u306a\u3063\u305f\u3002\u672c\u9175\u7d20\u306f1mM\u306e\u6c34\u9280\u30a4\u30aa\u30f3\u304a\u3088\u3073\u30d5\u30c3\u7d20\u30a4\u30aa\u30f3\u306e\u963b\u5bb3\u3092\u3046\u3051\u305f\u304c, \u9285\u30a4\u30aa\u30f3\u306b\u3088\u3063\u3066\u306f\u5168\u304f\u963b\u5bb3\u3055\u308c\u306a\u304b\u3063\u305f\u3002\u307e\u305f, \u81f3\u9069\u53cd\u5fdc\u6e29\u5ea6\u306f50\u2103\u306b\u3042\u3063\u3066, 10\u5206\u9593\u4fdd\u6301\u306e40\u2103\u307e\u3067\u306f\u5b89\u5b9a\u3067\u3042\u3063\u305f\u3002","subitem_description_type":"Other"},{"subitem_description":"The existence of an acid phosphatase in the mature cane stalks of NCO 376 variety, cultivated in Okinawa, was confirmed. The acid phosphatase hydrolyzing p-nitrophenyl phosphoric acid (NPP), from the extract of cane stalks, was partially purified by ammonium sulfate fractionation and acid treatment. This enzyme had an optimum pH of 5.5 and almost no activity at alkaline areas. It had Km value for NPP of 0.77mM and an optimum temperature of 50\u2103 and was stable at temperatures lower than 40\u2103. Magnesium, cupric and manganese ions in 1.16mM showed no effect on the enzyme activity. However, the inhibition of the activity by calcium and mercuric ions and EDTA in 1.16mM was remarkable, but that by fluoride ion in 1.16mM was moderate. EDTA and mercuric ion in 0.11mM, on the other hand, did not inactivate the enzyme. The modes of the inhibition by EDTA and calcium ion distinguish the enzyme studied in this work from the acid phosphatases obtained from several other plant species.","subitem_description_type":"Other"},{"subitem_description":"\u7d00\u8981\u8ad6\u6587","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"jpn"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/3963"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0370-4246"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN00250548"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"},{"subitem_1522650068558":"en","subitem_1522650091861":"The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1984-11-19","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"31","bibliographicPageEnd":"55","bibliographicPageStart":"51"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"KJ00000162203.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2002992/files/KJ00000162203.pdf"},"version_id":"aeef403d-a0ba-4b7b-9265-038bb9d3224a"}]},"item_title":"\u7518\u8517\u830e\u306e NPP- \u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u306e\u6027\u8cea(\u8fb2\u82b8\u5316\u5b66\u79d1)","item_type_id":"15","owner":"1","path":["1642837914150","1642838407312"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-02-14"},"publish_date":"2008-02-14","publish_status":"0","recid":"2002992","relation_version_is_last":true,"title":["\u7518\u8517\u830e\u306e NPP- \u30db\u30b9\u30d5\u30a1\u30bf\u30fc\u30bc\u306e\u6027\u8cea(\u8fb2\u82b8\u5316\u5b66\u79d1)"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T01:13:12.563089+00:00"}