{"created":"2022-01-27T02:46:03.485705+00:00","id":2003022,"links":{},"metadata":{"_buckets":{"deposit":"8d5233a8-8a79-420c-a266-d3153e44caeb"},"_deposit":{"id":"2003022","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2003022"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02003022","sets":["1642837622505:1642837905044:1642837913657","1642838403551:1642838407312"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"\u7d30\u83cc\u306e\u30a2\u30e9\u30d3\u30ca\u30f3\u5206\u89e3\u9175\u7d20\u306e\u7cbe\u88fd\u3068\u305d\u306e\u6027\u8cea(\u8fb2\u82b8\u5316\u5b66\u79d1)","subitem_1551255648112":"ja"},{"subitem_1551255647225":"Purification and properties of \u03b1-L-arabinofuranosidase I from Bacillus sp. (Department of Agricultural Chemistry)","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"\u5b89\u7530, \u6b63\u662d","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"\u5fb3\u91cc, \u653f\u4e08","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"\u702c\u5e95, \u6b63\u5eb7","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Yasuda, Masaaki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Tokuzato, Masatake","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sesoko, Masayasu","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"\u571f\u58cc\u304b\u3089\u5206\u96e2\u3057\u305f\u7d30\u83cc\u306e\u30a2\u30e9\u30d3\u30ca\u30f3\u5206\u89e3\u9175\u7d20\u306e\u7cbe\u88fd\u3092\u884c\u306a\u3044, \u305d\u306e\u6027\u8cea\u3092\u691c\u8a0e\u3057\u305f\u3002\u9175\u7d20\u306e\u7cbe\u88fd\u306f, Bacillus sp. No. 430\u306e\u57f9\u990a\u308d\u6db2\u304b\u3089\u786b\u9178\u30a2\u30f3\u30e2\u30cb\u30a6\u30e0\u5206\u753b, DEAE-\u30bb\u30eb\u30ed\u30fc\u30b9, QAE-\u30bb\u30d5\u30a1\u30c7\u30c3\u30af\u30b9A-50,DEAE-\u30c8\u30fc\u30e8\u30fc\u30d1\u30fc\u30eb650S, \u30d2\u30c9\u30ed\u30ad\u30b7\u30a2\u30d1\u30bf\u30a4\u30c8\u53ca\u3073\u30bb\u30d5\u30a1\u30c7\u30c3\u30af\u30b9G-75\u306a\u3069\u306e\u30ab\u30e9\u30e0\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u3092\u7d44\u307f\u5408\u308f\u305b\u3066\u884c\u306a\u3063\u305f\u3002\u7cbe\u88fd\u9175\u7d20\u306f\u30c7\u30a3\u30b9\u30af\u96fb\u6c17\u6cf3\u52d5\u7684\u306b\u9ad8\u5ea6\u306b\u7cbe\u88fd\u3055\u308c\u3066\u3044\u305f\u3002\u7cbe\u88fd\u9175\u7d20\u306e\u53cd\u5fdc\u81f3\u9069pH\u306f6.5,\u53cd\u5fdc\u81f3\u9069\u6e29\u5ea6\u306f40\u2103\u3067\u3042\u3063\u305f\u3002\u7cbe\u88fd\u9175\u7d20\u306f\u30d3\u30fc\u30c8\u30a2\u30e9\u30d3\u30ca\u30f3, \u30a2\u30e9\u30d3\u30ce\u30ac\u30e9\u30af\u30bf\u30f3, \u30a2\u30e9\u30d3\u30ce\u30ad\u30b7\u30e9\u30f3\u3084p-nitrophenyl \u03b1-L-arabinofuranoside\u306b\u4f5c\u7528\u3057\u305f\u3002\u53cd\u5fdc\u751f\u6210\u7269\u306fL-\u30a2\u30e9\u30d3\u30ce\u30fc\u30b9\u306e\u307f\u304c\u691c\u51fa\u3055\u308c\u305f\u3002\u672c\u7cbe\u88fd\u6a19\u54c1\u306f\u03b1-L-arabinofuranosidase\u3067\u3042\u308b\u3068\u8003\u3048\u3089\u308c\u305f\u3002","subitem_description_type":"Other"},{"subitem_description":"This study was carried out for a purpose of effective utilization of agricultural wastes by microbial enzymes. In order to liberate arabinose from arabinose-containing polysaccharides such as arabinan, arabinoxylan and arabinogalactan, an \u03b1-L-arabinofuranosidase was investigated. An \u03b1-L-arabinofuranosidase I was purified from the culture fluid of Bacillus sp. No. 430,which was isolated from the soil of sugar-cane fields. The process was as follows; salting out by ammonium sulfate, DEAE-cellulose, QAE-Sephadex A-50,DEAE-Toyopearl 650 S, hydroxyapatite and Sephadex G-75 column chromatography. The enzyme was highly purified by the method of discelectrophoresis. The purified enzyme had the maximum reactivity at pH 6.5 and 40\u2103. The highly purified enzyme was confirmed to be able to liberate L-arabinose from beet arabinan, arabinogalactan, arabinoxylan and p-nitrophenyl \u03b1-L-arabinofuranoside. The reaction product was paper chromatographically demonstrated to be only L-arabinose. The purified enzyme was inactive for p-nitrophenyl \u03b2-D-galctopyranoside.","subitem_description_type":"Other"},{"subitem_description":"\u7d00\u8981\u8ad6\u6587","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"jpn"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/3993"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0370-4246"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN00250548"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"},{"subitem_1522650068558":"en","subitem_1522650091861":"The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueNumber":"30","bibliographicPageEnd":"209","bibliographicPageStart":"201"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"KJ00000162167.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2003022/files/KJ00000162167.pdf"},"version_id":"e362bb1e-dcd5-474f-87d1-d5171f6e5de6"}]},"item_title":"\u7d30\u83cc\u306e\u30a2\u30e9\u30d3\u30ca\u30f3\u5206\u89e3\u9175\u7d20\u306e\u7cbe\u88fd\u3068\u305d\u306e\u6027\u8cea(\u8fb2\u82b8\u5316\u5b66\u79d1)","item_type_id":"15","owner":"1","path":["1642837913657","1642838407312"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-02-14"},"publish_date":"2008-02-14","publish_status":"0","recid":"2003022","relation_version_is_last":true,"title":["\u7d30\u83cc\u306e\u30a2\u30e9\u30d3\u30ca\u30f3\u5206\u89e3\u9175\u7d20\u306e\u7cbe\u88fd\u3068\u305d\u306e\u6027\u8cea(\u8fb2\u82b8\u5316\u5b66\u79d1)"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-02-24T00:43:50.479970+00:00"}