{"_buckets": {"deposit": "6770bb3a-5083-4ca9-a5cc-43ea42e02111"}, "_deposit": {"id": "2003029", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2003029"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02003029", "sets": ["1642837913657", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "サトウキビ茎の中性インベルターゼ(農芸化学科)", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Neutral invertase from sugar cane stalk (Department of Agricultural Chemistry)", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "仲宗根, 洋子", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "永友, 浩二郎", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Nakasone, Yoko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nagatomo, Kojiro", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "完熟サトウキビの搾汁液より硫安塩析した酵素蛋白画分を, インベルターゼ酵素標品として用い, その諸性質をしらべた。本酵素は, 最適pHを7.0附近にもつ中性インベルターゼであって, 最適pH5の酸性インベルターゼを, ほとんど含まなかった。本酵素はKm値が9.5mMで, シュクロースの分解率を1.0としたときにラフィノース, マルトースに対して, それぞれ, 0.24,0.11であった。本酵素はpCMB, 水銀イオンおよび銅イオンにより著しく阻害され, 搾汁中の耐熱性物質によっては中程度の阻害をうけた。またEDTAあるいはマグネシウムイオン, マンガンイオンなどの二価イオンは, 酵素活性にほとんど影響を及ぼさなかった。最適温度は35℃\u0026acd;40℃にあって, 温度に対する安定性では, 15分間保持の45℃までは安定であった。", "subitem_description_type": "Other"}, {"subitem_description": "The neutral invertase prepared from the mature sugar cane stalk by ammonium sulfate fractionation had Km value for sucrose of 9.5mM, a pH optimum of 7.0 and a low activity at acidic areas. It was completely inhibited by the presence of 10μM of PCMB or Hg^\u003c2+\u003e and 0.1mM of Cu^\u003c2+\u003e and was not affected by 1mM of EDTA, Mn^\u003c2+\u003e or Mg^\u003c2+\u003e. The enzyme was inactivated in as much as 40% by the thermal-, acid-stable substance from the sugar cane stalk or leaf. It had an optimum temperature of 35\u0026acd;40℃ and was stable at temperatures lower than 45℃, The enzyme mainly showed β-fructofuranosidase activity with small level of maltase activity.", "subitem_description_type": "Other"}, {"subitem_description": "紀要論文", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "琉球大学農学部"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/4000"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "琉球大学農学部学術報告"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1983-11-19", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "30", "bibliographicPageEnd": "245", "bibliographicPageStart": "241"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00000162174.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2003029/files/KJ00000162174.pdf"}, "version_id": "3fe9562a-51c6-4373-a22a-81274ac84dac"}]}, "item_title": "サトウキビ茎の中性インベルターゼ(農芸化学科)", "item_type_id": "15", "owner": "1", "path": ["1642837913657", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/4000", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2003029", "relation": {}, "relation_version_is_last": true, "title": ["サトウキビ茎の中性インベルターゼ(農芸化学科)"], "weko_shared_id": -1}