{"_buckets": {"deposit": "6770bb3a-5083-4ca9-a5cc-43ea42e02111"}, "_deposit": {"id": "2003029", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2003029"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02003029", "sets": ["1642837913657", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "\u30b5\u30c8\u30a6\u30ad\u30d3\u830e\u306e\u4e2d\u6027\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc(\u8fb2\u82b8\u5316\u5b66\u79d1)", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Neutral invertase from sugar cane stalk (Department of Agricultural Chemistry)", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u4ef2\u5b97\u6839, \u6d0b\u5b50", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u6c38\u53cb, \u6d69\u4e8c\u90ce", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Nakasone, Yoko", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nagatomo, Kojiro", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "\u5b8c\u719f\u30b5\u30c8\u30a6\u30ad\u30d3\u306e\u643e\u6c41\u6db2\u3088\u308a\u786b\u5b89\u5869\u6790\u3057\u305f\u9175\u7d20\u86cb\u767d\u753b\u5206\u3092, \u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u9175\u7d20\u6a19\u54c1\u3068\u3057\u3066\u7528\u3044, \u305d\u306e\u8af8\u6027\u8cea\u3092\u3057\u3089\u3079\u305f\u3002\u672c\u9175\u7d20\u306f, \u6700\u9069pH\u30927.0\u9644\u8fd1\u306b\u3082\u3064\u4e2d\u6027\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u3067\u3042\u3063\u3066, \u6700\u9069pH5\u306e\u9178\u6027\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u3092, \u307b\u3068\u3093\u3069\u542b\u307e\u306a\u304b\u3063\u305f\u3002\u672c\u9175\u7d20\u306fKm\u5024\u304c9.5mM\u3067, \u30b7\u30e5\u30af\u30ed\u30fc\u30b9\u306e\u5206\u89e3\u7387\u30921.0\u3068\u3057\u305f\u3068\u304d\u306b\u30e9\u30d5\u30a3\u30ce\u30fc\u30b9, \u30de\u30eb\u30c8\u30fc\u30b9\u306b\u5bfe\u3057\u3066, \u305d\u308c\u305e\u308c, 0.24,0.11\u3067\u3042\u3063\u305f\u3002\u672c\u9175\u7d20\u306fpCMB, \u6c34\u9280\u30a4\u30aa\u30f3\u304a\u3088\u3073\u9285\u30a4\u30aa\u30f3\u306b\u3088\u308a\u8457\u3057\u304f\u963b\u5bb3\u3055\u308c, \u643e\u6c41\u4e2d\u306e\u8010\u71b1\u6027\u7269\u8cea\u306b\u3088\u3063\u3066\u306f\u4e2d\u7a0b\u5ea6\u306e\u963b\u5bb3\u3092\u3046\u3051\u305f\u3002\u307e\u305fEDTA\u3042\u308b\u3044\u306f\u30de\u30b0\u30cd\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3, \u30de\u30f3\u30ac\u30f3\u30a4\u30aa\u30f3\u306a\u3069\u306e\u4e8c\u4fa1\u30a4\u30aa\u30f3\u306f, \u9175\u7d20\u6d3b\u6027\u306b\u307b\u3068\u3093\u3069\u5f71\u97ff\u3092\u53ca\u307c\u3055\u306a\u304b\u3063\u305f\u3002\u6700\u9069\u6e29\u5ea6\u306f35\u2103\u0026acd;40\u2103\u306b\u3042\u3063\u3066, \u6e29\u5ea6\u306b\u5bfe\u3059\u308b\u5b89\u5b9a\u6027\u3067\u306f, 15\u5206\u9593\u4fdd\u6301\u306e45\u2103\u307e\u3067\u306f\u5b89\u5b9a\u3067\u3042\u3063\u305f\u3002", "subitem_description_type": "Other"}, {"subitem_description": "The neutral invertase prepared from the mature sugar cane stalk by ammonium sulfate fractionation had Km value for sucrose of 9.5mM, a pH optimum of 7.0 and a low activity at acidic areas. It was completely inhibited by the presence of 10\u03bcM of PCMB or Hg^\u003c2+\u003e and 0.1mM of Cu^\u003c2+\u003e and was not affected by 1mM of EDTA, Mn^\u003c2+\u003e or Mg^\u003c2+\u003e. The enzyme was inactivated in as much as 40% by the thermal-, acid-stable substance from the sugar cane stalk or leaf. It had an optimum temperature of 35\u0026acd;40\u2103 and was stable at temperatures lower than 45\u2103, The enzyme mainly showed \u03b2-fructofuranosidase activity with small level of maltase activity.", "subitem_description_type": "Other"}, {"subitem_description": "\u7d00\u8981\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/4000"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "30", "bibliographicPageEnd": "245", "bibliographicPageStart": "241"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00000162174.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2003029/files/KJ00000162174.pdf"}, "version_id": "3fe9562a-51c6-4373-a22a-81274ac84dac"}]}, "item_title": "\u30b5\u30c8\u30a6\u30ad\u30d3\u830e\u306e\u4e2d\u6027\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc(\u8fb2\u82b8\u5316\u5b66\u79d1)", "item_type_id": "15", "owner": "1", "path": ["1642837913657", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/4000", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2003029", "relation": {}, "relation_version_is_last": true, "title": ["\u30b5\u30c8\u30a6\u30ad\u30d3\u830e\u306e\u4e2d\u6027\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc(\u8fb2\u82b8\u5316\u5b66\u79d1)"], "weko_shared_id": -1}