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  1. 紀要論文
  2. 琉球大学農学部学術報告
  3. 30号
  1. 部局別インデックス
  2. 農学部

サトウキビ茎の中性インベルターゼ(農芸化学科)

http://hdl.handle.net/20.500.12000/4000
http://hdl.handle.net/20.500.12000/4000
d98785ff-7af0-4ac0-8411-14350e8ffd84
名前 / ファイル ライセンス アクション
KJ00000162174.pdf KJ00000162174.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2008-02-14
タイトル
タイトル サトウキビ茎の中性インベルターゼ(農芸化学科)
言語 ja
作成者 仲宗根, 洋子

× 仲宗根, 洋子

ja 仲宗根, 洋子

永友, 浩二郎

× 永友, 浩二郎

ja 永友, 浩二郎

Nakasone, Yoko

× Nakasone, Yoko

en Nakasone, Yoko

Nagatomo, Kojiro

× Nagatomo, Kojiro

en Nagatomo, Kojiro

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
内容記述
内容記述タイプ Other
内容記述 完熟サトウキビの搾汁液より硫安塩析した酵素蛋白画分を, インベルターゼ酵素標品として用い, その諸性質をしらべた。本酵素は, 最適pHを7.0附近にもつ中性インベルターゼであって, 最適pH5の酸性インベルターゼを, ほとんど含まなかった。本酵素はKm値が9.5mMで, シュクロースの分解率を1.0としたときにラフィノース, マルトースに対して, それぞれ, 0.24,0.11であった。本酵素はpCMB, 水銀イオンおよび銅イオンにより著しく阻害され, 搾汁中の耐熱性物質によっては中程度の阻害をうけた。またEDTAあるいはマグネシウムイオン, マンガンイオンなどの二価イオンは, 酵素活性にほとんど影響を及ぼさなかった。最適温度は35℃∿40℃にあって, 温度に対する安定性では, 15分間保持の45℃までは安定であった。
内容記述タイプ Other
内容記述 The neutral invertase prepared from the mature sugar cane stalk by ammonium sulfate fractionation had Km value for sucrose of 9.5mM, a pH optimum of 7.0 and a low activity at acidic areas. It was completely inhibited by the presence of 10μM of PCMB or Hg^<2+> and 0.1mM of Cu^<2+> and was not affected by 1mM of EDTA, Mn^<2+> or Mg^<2+>. The enzyme was inactivated in as much as 40% by the thermal-, acid-stable substance from the sugar cane stalk or leaf. It had an optimum temperature of 35&acd;40℃ and was stable at temperatures lower than 45℃, The enzyme mainly showed β-fructofuranosidase activity with small level of maltase activity.
内容記述タイプ Other
内容記述 紀要論文
出版者
言語 ja
出版者 琉球大学農学部
言語
言語 jpn
資源タイプ
資源タイプ departmental bulletin paper
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
識別子
識別子 http://hdl.handle.net/20.500.12000/4000
識別子タイプ HDL
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0370-4246
収録物識別子タイプ NCID
収録物識別子 AN00250548
収録物名
言語 ja
収録物名 琉球大学農学部学術報告
書誌情報
号 30, p. 241-245
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