{"created":"2022-01-27T02:46:58.158515+00:00","id":2003052,"links":{},"metadata":{"_buckets":{"deposit":"c9f5a761-cce3-46ba-8407-ef9d074dccb8"},"_deposit":{"id":"2003052","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2003052"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02003052","sets":["1642837622505:1642837905044:1642837913242","1642838403551:1642838407312"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"Corynebacterium sepedonicum の結晶 L-オルニチントランスアミナーゼ : 反応生成物の同定(農芸化学科)","subitem_title_language":"ja"},{"subitem_title":"Crystalline L-ornithine transaminase from Corynebacterium sepedonicum : Identification of reaction product(Department of Agricultural Chemistry)","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"安田, 正昭","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"当山, 清善","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"与那覇, 和雄","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"左右田, 健次","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Yasuda, Masaaki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Toyama, Seizen","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yonaha, Kazuo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Soda, Kenji","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Corynebacterium sepedonicum (IFO 3306)のL-オルニチントランスアミナーゼ(結晶標品)のL-オルニチンに対する作用特性を明らかにした。酵素反応によりL-オルニチンに由来する反応生成物は, イオン交換クロマトグラフィー及び高圧濾紙電気泳動などの分析結果から⊿^1-ピロリン-5-カルボン酸であることが確められた。一方, α-ケトグルタル酸からはL-グルタミン酸が生成された。これらのことから, 本酵素はL-オルニチンの末端アミノ基とα-ケトグルタル酸との間のアミノ基転移反応を触媒し, L-グルタミン酸とグルタミン酸-γ-セミアルデヒドを生成するが, 後者は非酵素的に脱水閉環して⊿^1-ピロリン-5-カルボン酸に変化することが明らかになった。","subitem_description_type":"Other"},{"subitem_description":"The reaction product of L-ornithine with crystalline L-ornithine transaminase from Corynebacterium sepedonicum (IFO 3306) was investigated. The formation of ⊿^1-pyrroline-5-carboxylate was shown by amino acid analysis and paper electrophoresis of the reaction product. These findings revealed that the terminal amino group of L-ornithine is transaminated to yield ⊿^1-pyrroline-5-carboxylate. Therefore, glutamate-γ-semialdehyde is produced directly from L-ornithine. Glutamate formed was identified as the L-enantiomer with L-glutamate dehydrogenase.","subitem_description_type":"Other"},{"subitem_description":"紀要論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"琉球大学農学部","subitem_publisher_language":"ja"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/4023"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"0370-4246","subitem_source_identifier_type":"ISSN"},{"subitem_source_identifier":"AN00250548","subitem_source_identifier_type":"NCID"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_source_title":"琉球大学農学部学術報告","subitem_source_title_language":"ja"},{"subitem_source_title":"The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus","subitem_source_title_language":"en"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1982-12-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"29","bibliographicPageEnd":"52","bibliographicPageStart":"47"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"KJ00000162132.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2003052/files/KJ00000162132.pdf"},"version_id":"b4439333-f5d0-481f-88cf-4aa23aff90e2"}]},"item_title":"Corynebacterium sepedonicum の結晶 L-オルニチントランスアミナーゼ : 反応生成物の同定(農芸化学科)","item_type_id":"15","owner":"1","path":["1642837913242","1642838407312"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-02-14"},"publish_date":"2008-02-14","publish_status":"0","recid":"2003052","relation_version_is_last":true,"title":["Corynebacterium sepedonicum の結晶 L-オルニチントランスアミナーゼ : 反応生成物の同定(農芸化学科)"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T01:14:27.278660+00:00"}