{"created":"2022-01-27T02:47:05.754658+00:00","id":2003056,"links":{},"metadata":{"_buckets":{"deposit":"13be7bbb-ff7f-4780-a840-46f5f6a8f2fd"},"_deposit":{"id":"2003056","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2003056"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02003056","sets":["1642837622505:1642837905044:1642837913242","1642838403551:1642838407312"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"\u30af\u30ef\u30ba\u30a4\u30e2\u8449\u306e\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc(\u8fb2\u82b8\u5316\u5b66\u79d1)","subitem_1551255648112":"ja"},{"subitem_1551255647225":"Invertase from Alocasia leaves(Department of Agricultural Chemistry)","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"\u4ef2\u5b97\u6839, \u6d0b\u5b50","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"\u5b89\u4e95, \u52dd","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Nakasone, Yoko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yasui, Masaru","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"\u30af\u30ef\u30ba\u30a4\u30e2\u8449\u306e\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u306e\u8abf\u88fd\u65b9\u6cd5\u3068\u3057\u3066\u306f, \u786b\u5b89\u5869\u6790\u3088\u308a\u3082\u30a2\u30bb\u30c8\u30f3\u6c88\u6fb1\u6cd5\u304c\u52b9\u679c\u7684\u3067\u3042\u3063\u305f\u3002\u305d\u3053\u3067, 20&acd;40%\u30a2\u30bb\u30c8\u30f3\u6fc3\u5ea6\u3067\u6c88\u6fb1\u3057\u305f, \u90e8\u5206\u7cbe\u88fd\u306e\u86cb\u767d\u753b\u5206\u3092, \u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u9175\u7d20\u6a19\u54c1\u3068\u3057\u3066\u7528\u3044, \u305d\u306e\u8af8\u6027\u8cea\u3092\u691c\u8a0e\u3057\u305f\u3002\u672c\u9175\u7d20\u306f\u81f3\u9069pH\u304c5.0\u3067km\u5024\u304c9mM\u306e\u9178\u6027\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc\u3067\u3042\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u3063\u305f\u3002\u672c\u9175\u7d20\u306f1mM\u6c34\u9280\u30a4\u30aa\u30f3\u306b\u3088\u308a\u307b\u307c\u5b8c\u5168\u306b\u5931\u6d3b\u3057, \u30de\u30f3\u30ac\u30f3\u30a4\u30aa\u30f3\u304a\u3088\u3073\u30de\u30b0\u30cd\u30b7\u30a6\u30e0\u30a4\u30aa\u30f3\u306e\u963b\u5bb3\u3092\u3082\u3046\u3051\u305f\u3002\u307e\u305f, \u81f3\u9069\u6e29\u5ea6\u306f35\u2103\u306b\u3042\u3063\u3066, 15\u5206\u9593\u4fdd\u6301\u306e45\u2103\u4ee5\u4e0a\u306e\u6e29\u5ea6\u3067\u306f\u307b\u3068\u3093\u3069\u6d3b\u6027\u3092\u5931\u306a\u3063\u305f\u3002","subitem_description_type":"Other"},{"subitem_description":"The paper reports the presence and some properties of acid invertase from Alocasia odora C. Koch leaves. Invertase activities for the protein fractions obtained by acetone, ethanol or ammonium sulfate precipitations were determined. The specific activities of acetone fraction, ethanol fraction and ammonium sulfate fraction were 11.9,14.3 and 2.2,respectively, indicating that the activities of the fractions obtained by the two organic solvents were about six times higher than that obtained by ammonium sulfate. Acetone fraction had an almost equivalent value to that of ethanol fraction in specific activity and the former had two times more than the latter in the content of enzyme protein. The fraction precipitated under 20-40% concentration of acetone, containing most of the invertase, was used for examination of the properties of the invertase from alocasia leaves. The invertase present in the leaves was acid invertase, having a pH optimum of 5.0 and almost no activity at alkaline areas. It had an optimum temperature of 35\u2103 and was inactivated at temperatures higher than 45\u2103. The enzyme was inhibited by the presence of 1 mM of Hg^<2+>, Mn^<2+> and Mg^<2+> and was not affected by that of Ca^<2+>, Fe^<2+>, Co^<2+> and K^+. The enzyme mainly showed \u03b2-fructofuranosidase activity with small level of maltase activity.","subitem_description_type":"Other"},{"subitem_description":"\u7d00\u8981\u8ad6\u6587","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"jpn"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/4027"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0370-4246"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN00250548"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"},{"subitem_1522650068558":"en","subitem_1522650091861":"The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueNumber":"29","bibliographicPageEnd":"78","bibliographicPageStart":"73"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"KJ00000162136.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2003056/files/KJ00000162136.pdf"},"version_id":"30217bd3-6a53-4ae8-a050-3d1315aa7e47"}]},"item_title":"\u30af\u30ef\u30ba\u30a4\u30e2\u8449\u306e\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc(\u8fb2\u82b8\u5316\u5b66\u79d1)","item_type_id":"15","owner":"1","path":["1642837913242","1642838407312"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-02-14"},"publish_date":"2008-02-14","publish_status":"0","recid":"2003056","relation_version_is_last":true,"title":["\u30af\u30ef\u30ba\u30a4\u30e2\u8449\u306e\u30a4\u30f3\u30d9\u30eb\u30bf\u30fc\u30bc(\u8fb2\u82b8\u5316\u5b66\u79d1)"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-02-15T05:29:20.230999+00:00"}