{"_buckets": {"deposit": "bfb9d690-d50c-4dbf-b234-ec7abcae889a"}, "_deposit": {"id": "2003079", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2003079"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02003079", "sets": ["1642837912813", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "未熟サトウキビ茎のグリコシダーゼに関する研究(農芸化学科)", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Studies on Glycosidases of Immature Sugar Cane Stalks (Department of Agricultural Chemistry)", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "知念, 功", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "仲村, 毅", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "玉城, 一", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "嶺井, 勝", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "真下, 均", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "福田, 亘博", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Chinen, Isao", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nakamura, Tuyoshi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Tamaki, Hajime", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Minei, Masaru", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Mashimo, Hitoshi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Fukuda, Nobuhiro", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "用いた若いサトウキビの茎の直径, 重さ, 糖度を各節ごとに調べた結果, 基部では, すべてその数値が大きく, 頂部にいくにつれ小さくなった。その長さは, 第3節で最も長かった。ヘキソース濃度は, 第4節でピークになった。蔗糖濃度は, 第4節から基部へ向かって急激に高くなった。この茎には, α-ガラクトシダーゼ, α-マシノシダーゼ, インベルターゼ, β-N-アセチルグルコサミダーゼ, β-グルコシダーゼ, β-キシロシダーゼ等のグリコシダーゼが存在し, これらのグリコシダーゼ濃度は, 第4節附近でピークをなしていた。これらのグリコシダーゼを精製する目的で, 蔗汁からの脱色法を検討した結果, DEAEセルロースカラムを用いると脱色効果が大で, 蛋白質やこれらのグリコシダーゼの吸着が殆んどないことがわかった。またα-マンノシダーゼ, α-ガラクトシダーゼおよびβ-N-アセチルグリコサミダーゼは, 或る程度精製すると, ディスクゲル電気泳動にかけたのち, ゲル中の酵素活性を発色させることができた。", "subitem_description_type": "Other"}, {"subitem_description": "The diameter, weight and sugar concentration of joints of immature sugar cane stalk were largest at the base, and gradually decreased to the top. But the lenghth and the hexose concentration were largest at the 4th joint from top of the stalk. Various glycosidase such as α-galactosidase, α-mannosidase, invertase, β-N-acetyglucosaminidase, β-glucosidase, and β-xylosidase occured in this plant, and these concentrations were largest at the 4th joint. Decolorization from the juice was studied as a preliminary experiment to purify these glycosidases. Application of the juice to the DEAE-cellulose column previously equlibrated with 1/100M citrate buffer (pH 5.5) was effective in juice clarification. Protein and these glycosidases were not adsorbed. After purification to some extent, α-galactosidase, α-mannosidase, and β-N-acetylglucosaminidase were applied to polyacrylamide gel electrophoresis and located in the gel by staining for enzymatic activity by using naphthyl glycopyranosides as substrate.", "subitem_description_type": "Other"}, {"subitem_description": "紀要論文", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "琉球大学農学部"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/4050"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "琉球大学農学部学術報告"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1981-11-30", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "28", "bibliographicPageEnd": "80", "bibliographicPageStart": "73"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00000162091.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2003079/files/KJ00000162091.pdf"}, "version_id": "a81c809f-2a6d-478c-9fe9-2db330b2231d"}]}, "item_title": "未熟サトウキビ茎のグリコシダーゼに関する研究(農芸化学科)", "item_type_id": "15", "owner": "1", "path": ["1642837912813", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/4050", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2003079", "relation": {}, "relation_version_is_last": true, "title": ["未熟サトウキビ茎のグリコシダーゼに関する研究(農芸化学科)"], "weko_shared_id": -1}