{"_buckets": {"deposit": "cf471df9-6620-4ffc-8837-c96721a943be"}, "_deposit": {"id": "2003080", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2003080"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02003080", "sets": ["1642837912813", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "泡盛麹菌における L-アラニン : α-ケトグルタル酸トランスアミナーゼの失活現象(農芸化学科)", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Inactivation of L-alanine : α-Ketoglutarate transaminase in Aspergillus awamori (Department of Agricultural Chemistry)", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "石原, 昌信", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "与那覇, 和雄", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "当山, 清善", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Ishihara, Masanobu", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Yonaha, Kazuo", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Toyama, Seizen", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "供試泡盛麹菌(Aspergillus awamori)を蔗糖・ペプトン含有培地で培養を行ない, 得られた菌体の無細胞抽出液についてL-アラニン : α-ケトグルタル酸トランスアミナーゼ活性を調べた結果, 培養24時間目で最も高い活性が得られた。本酵素は不安定であったが, 本酵素をピリドキサールー5\u0027-リン酸(PLP)と2-メルカプトエタノールとともに加温処理(37℃, 30分間)すると安定化した。本酵素は硫酸アンモニウム塩析で失活が起った。本失活酵素はPLP及び2-メルカプトエタノールとともに加温処理すると活性の回復がみられた。また, 本酵素はPLPまたは2-メルカプトエタノール非存在下ではP-クロルマーキュリ安息香酸によって失活をうけた。", "subitem_description_type": "Other"}, {"subitem_description": "When Aspergillus awamori IFO 4122 was grown on sucrose-peptone medium for 24 hr under shaking, a hight activity of the transaminase was found in the cell-free extract. Both pyridoxal-5\u0027-phosphate (PLP) and 2-mercaptoethanol protected the enzyme from heat inactivation. The inactivated enzyme by ammonium sulphate fractionation was activated by preincubation with PLP and 2-mercaptoethanol. The enzyme was inactivated with p-chloromer-curibenzoate in the absence of PLP or 2-mercaptoethanol.", "subitem_description_type": "Other"}, {"subitem_description": "紀要論文", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "琉球大学農学部"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/4051"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "琉球大学農学部学術報告"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1981-11-30", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "28", "bibliographicPageEnd": "88", "bibliographicPageStart": "81"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00000162092.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2003080/files/KJ00000162092.pdf"}, "version_id": "f911de51-2d11-4fda-9965-bdd1e0d3480a"}]}, "item_title": "泡盛麹菌における L-アラニン : α-ケトグルタル酸トランスアミナーゼの失活現象(農芸化学科)", "item_type_id": "15", "owner": "1", "path": ["1642837912813", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/4051", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2003080", "relation": {}, "relation_version_is_last": true, "title": ["泡盛麹菌における L-アラニン : α-ケトグルタル酸トランスアミナーゼの失活現象(農芸化学科)"], "weko_shared_id": -1}