{"created":"2022-01-27T02:47:53.469785+00:00","id":2003082,"links":{},"metadata":{"_buckets":{"deposit":"cef79333-2c10-4793-96f3-970653caf13b"},"_deposit":{"id":"2003082","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2003082"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02003082","sets":["1642837622505:1642837905044:1642837912813","1642838403551:1642838407312"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"Corynebacterium sepedonicum \u306e L-\u30aa\u30eb\u30cb\u30c1\u30f3\u30c8\u30e9\u30f3\u30b9\u30a2\u30df\u30ca\u30fc\u30bc(\u8fb2\u82b8\u5316\u5b66\u79d1)","subitem_1551255648112":"ja"},{"subitem_1551255647225":"L-Ornithine Aminotransferase from Corynebacterium sepedonicum (Department of Agricultural Chemistry)","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"\u5b89\u7530, \u6b63\u662d","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"\u5f53\u5c71, \u6e05\u5584","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"\u4e0e\u90a3\u8987, \u548c\u96c4","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"\u5de6\u53f3\u7530, \u5065\u6b21","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Yasuda, Masaaki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Toyama, Seizen","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yonaha, Kazuo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Soda, Kenji","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"Corynebacterium sepedonicum(IFO 3306)\u306e\u7121\u7d30\u80de\u62bd\u51fa\u6db2\u4e2d\u306bL-\u30aa\u30eb\u30cb\u30c1\u30f3\u3092\u30a2\u30df\u30ce\u57fa\u4f9b\u4e0e\u4f53\u3068\u3057\u305f\u30a2\u30df\u30ce\u57fa\u8ee2\u79fb\u53cd\u5fdc\u3092\u89e6\u5a92\u3059\u308b\u30c8\u30e9\u30f3\u30b9\u30a2\u30df\u30ca\u30fc\u30bc\u306e\u5b58\u5728\u304c\u660e\u3089\u304b\u306b\u306a\u3063\u305f\u3002\u6700\u3082\u9ad8\u3044\u9175\u7d20\u6d3b\u6027\u306f, \u672c\u83cc\u682a\u3092L-\u30aa\u30eb\u30cb\u30c1\u30f3(0.3%)\u307e\u305f\u306fL-\u30a2\u30eb\u30ae\u30cb\u30f3\u3092\u6dfb\u52a0\u3057\u305f\u57f9\u5730(\u30da\u30d7\u30c8\u30f3, \u30b0\u30ea\u30bb\u30ea\u30f3\u3092\u542b\u3080)\u3067pH7.2,30\u2103, 18-20\u6642\u9593\u632f\u3068\u3046\u57f9\u990a\u3057\u3066\u5f97\u305f\u83cc\u4f53\u3067\u5f97\u3089\u308c\u305f\u3002\u672c\u9175\u7d20\u306f\u57f9\u5730\u4e2d\u306eL-\u30aa\u30eb\u30cb\u30c1\u30f3\u307e\u305f\u306fL-\u30a2\u30eb\u30ae\u30cb\u30f3\u306b\u3088\u308a\u8a98\u5c0e\u7684\u306b\u751f\u6210\u3055\u308c\u305f\u3002\u672c\u9175\u7d20\u306fL-\u30aa\u30eb\u30cb\u30c1\u30f3\u3068\u03b1-\u30b1\u30c8\u30b0\u30eb\u30bf\u30eb\u9178\u3068\u306e\u9593\u306e\u30a2\u30df\u30ce\u57fa\u8ee2\u79fb\u53cd\u5fdc\u3092\u89e6\u5a92\u3057L-\u30b0\u30eb\u30bf\u30df\u30f3\u9178\u3092\u751f\u6210\u3057, \u5316\u5b66\u91cf\u8ad6\u7684\u306b\u53cd\u5fdc\u304c\u9032\u884c\u3057\u305f\u3002\u672c\u9175\u7d20\u306e\u53cd\u5fdc\u6700\u9069pH\u306f8.0\u3067, \u53cd\u5fdc\u6700\u9069\u6e29\u5ea6\u306f45\u2103\u3067\u3042\u3063\u305f\u3002","subitem_description_type":"Other"},{"subitem_description":"The production and some properties of the enzyme which catalyzes transamination between L-ornithine and \u03b1-ketoglutarate were described. The enzyme was found in the cell-free extract of Corynebacterium sepedonicum (IFO 3306). In order to find the medium conditions required for the improvement of the enzyme production by this strain, the organism was grown in the medium contaning L-ornithine or L-arginine. The best enzyme production was obtained when the organism was grown in the medium (initial pH 7.2) contaning L-ornithine (0.3%) at 30\u2103 for 18-20hr under aerobic conditions. The cell-free extract of the strain grown under the conditions mentioned above catalyzed the transamination reaction between L-ornithine and \u03b1-ketoglutarate to produce L-glutamate. This enzymatic transamination was found to proceed stoichiometrically. The enzyme had the maximum reactivity at pH 8.0 and 45\u2103 for the transamination reaction.","subitem_description_type":"Other"},{"subitem_description":"\u7d00\u8981\u8ad6\u6587","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"jpn"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/4053"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0370-4246"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN00250548"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"},{"subitem_1522650068558":"en","subitem_1522650091861":"The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueNumber":"28","bibliographicPageEnd":"109","bibliographicPageStart":"101"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"KJ00000162094.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2003082/files/KJ00000162094.pdf"},"version_id":"63533290-a2a8-4027-9d85-c83db813ad07"}]},"item_title":"Corynebacterium sepedonicum \u306e L-\u30aa\u30eb\u30cb\u30c1\u30f3\u30c8\u30e9\u30f3\u30b9\u30a2\u30df\u30ca\u30fc\u30bc(\u8fb2\u82b8\u5316\u5b66\u79d1)","item_type_id":"15","owner":"1","path":["1642837912813","1642838407312"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-02-14"},"publish_date":"2008-02-14","publish_status":"0","recid":"2003082","relation_version_is_last":true,"title":["Corynebacterium sepedonicum \u306e L-\u30aa\u30eb\u30cb\u30c1\u30f3\u30c8\u30e9\u30f3\u30b9\u30a2\u30df\u30ca\u30fc\u30bc(\u8fb2\u82b8\u5316\u5b66\u79d1)"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-02-15T05:29:51.132280+00:00"}