{"created":"2022-01-27T02:48:53.010674+00:00","id":2003114,"links":{},"metadata":{"_buckets":{"deposit":"47bd4cb2-ffee-4b92-8380-8c8a0d5b319e"},"_deposit":{"id":"2003114","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2003114"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02003114","sets":["1642837622505:1642837905044:1642837912269","1642838403551:1642838407312"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"\u30cf\u30d6\u6bd2\u306e\u30b3\u30e9\u30b2\u30ca\u30fc\u30bc\u306b\u95a2\u3059\u308b\u7814\u7a76(\u8fb2\u82b8\u5316\u5b66\u79d1)","subitem_1551255648112":"ja"},{"subitem_1551255647225":"Studies on collagenase of Habu (Trimeresurus flavoviridis) venom (Department of Agricultural Chemistry)","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"\u77e5\u5ff5, \u529f","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"\u77f3\u677e, \u4eae","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"\u798f\u7530, \u4e98\u535a","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Chinen, Isao","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Ishimatsu, Akira","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Fukuda, Nobuhiro","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"\u30cf\u30d6\u6bd2\u3092\u4e0d\u6eb6\u6027\u30b3\u30e9\u30fc\u30b2\u30f3\u306b\u4f5c\u7528\u3057, \u751f\u6210\u3057\u3066\u304f\u308b\u53ef\u6eb6\u6027\u30da\u30d7\u30bf\u30a4\u30c9\u4e2d\u306e\u30d2\u30c9\u30ed\u30ad\u30b7\u30d7\u30ed\u30ea\u30f3\u542b\u91cf\u3092\u5b9a\u91cf\u3059\u308b\u3053\u3068\u306b\u3088\u308a, \u30cf\u30d6\u6bd2\u30b3\u30e9\u30b2\u30ca\u30fc\u30bc\u6d3b\u6027\u3092\u6c42\u3081\u305f\u3002\u3053\u306e\u9175\u7d20\u306e\u81f3\u9069pH\u306f, 8.0\u3067\u3042\u308a, \u81f3\u9069\u6e29\u5ea6\u306f, 60\u2103\u3067\u3042\u3063\u305f\u3002\u3053\u306e\u4e0d\u6eb6\u6027\u30b3\u30e9\u30fc\u30b2\u30f3\u306b\u3053\u306e\u9175\u7d20\u3092\u5438\u7740\u3055\u305b(30\u2103, 60\u5206\u53cd\u5fdc), \u9060\u5fc3\u3057, 1M\u98df\u5869\u6eb6\u6db2\u3067\u6eb6\u51fa\u3057, \u672c\u9175\u7d20\u3092\u7cbe\u88fd\u3057\u305f\u3002\u672c\u9175\u7d20\u306e\u5438\u7740\u7387\u306f, 80%\u3067\u3042\u3063\u305f\u3002\u307e\u305f\u6eb6\u51fa\u7387\u308280%\u3067\u3042\u3063\u305f\u3002\u3053\u306e\u30b3\u30e9\u30fc\u30b2\u30f3\u3068\u7c97\u6bd2\u306e\u6dfb\u52a0\u5272\u5408\u306f, 300 : 1\u306e\u6642\u672c\u9175\u7d20\u306e\u5438\u7740\u7387\u306f, \u6700\u5927\u3067\u3042\u3063\u305f\u3002\u3053\u306e\u7cbe\u88fd\u9175\u7d20\u3092\u3055\u3089\u306b\u30bb\u30d5\u30a1\u30c7\u30c3\u30af\u30b9G-75,G-150\u30b2\u30eb\u30ab\u30e9\u30e0\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u306b\u304b\u3051\u7d14\u5316\u3057\u305f\u7d50\u679c, \u305d\u306e\u7d14\u5316\u500d\u7387\u306f, \u7d041.13\u500d\u306b\u306a\u3063\u305f\u3002\u3053\u306e\u7d14\u5316\u9175\u7d20\u306f, \u30c7\u30a3\u30b9\u30af\u30dd\u30ea\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u7684\u306b2\u500b\u4ee5\u4e0a\u306e\u30d0\u30f3\u30c9\u304c\u898b\u3089\u308c, \u307e\u305f\u30b3\u30e9\u30b2\u30ca\u30fc\u30bc\u6d3b\u6027\u4ee5\u5916\u306b\u30ab\u30bc\u30a4\u30f3\u6c34\u89e3\u6d3b\u6027\u3082\u898b\u3089\u308c\u305f\u3002","subitem_description_type":"Other"},{"subitem_description":"Collagenase activity of Habu venom was measured the amount of hydroxyproline of soluble peptide liberated by reaction of the venom and insoluble collagen (60\u2103, 30 min). The enzyme activity was maximum at pH 8.0 and at 60\u2103. The purification of the enzyme was carried out by being adsorbed onto insoluble collagen (30\u2103, 60min), centrifuged, and eluted with 1M sodium cloride solution. Rate of adsorption of the enzyme was maximum, 80% for total activity, when ratio of insoluble collagen to the enzyme was 300 : 1,and the elution also 80% for activity of the enzyme adsorbed. Further purification of the enzyme fraction by Sephadex G-75 and G-150 column chromatography was found to be a 6.5% over all yield and a 1.13 fold increase in specific activity. The purified enzyme revealed two more bands by disc gel electrophoresis and hydrolysed casein.","subitem_description_type":"Other"},{"subitem_description":"\u7d00\u8981\u8ad6\u6587","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"jpn"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/4085"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0370-4246"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN00250548"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"},{"subitem_1522650068558":"en","subitem_1522650091861":"The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueNumber":"27","bibliographicPageEnd":"56","bibliographicPageStart":"43"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"KJ00000162043.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2003114/files/KJ00000162043.pdf"},"version_id":"a5e8873d-fdef-47a3-b993-3a90705537d0"}]},"item_title":"\u30cf\u30d6\u6bd2\u306e\u30b3\u30e9\u30b2\u30ca\u30fc\u30bc\u306b\u95a2\u3059\u308b\u7814\u7a76(\u8fb2\u82b8\u5316\u5b66\u79d1)","item_type_id":"15","owner":"1","path":["1642837912269","1642838407312"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-02-14"},"publish_date":"2008-02-14","publish_status":"0","recid":"2003114","relation_version_is_last":true,"title":["\u30cf\u30d6\u6bd2\u306e\u30b3\u30e9\u30b2\u30ca\u30fc\u30bc\u306b\u95a2\u3059\u308b\u7814\u7a76(\u8fb2\u82b8\u5316\u5b66\u79d1)"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-02-15T05:30:28.805563+00:00"}