{"created":"2022-01-27T02:48:54.798501+00:00","id":2003115,"links":{},"metadata":{"_buckets":{"deposit":"5936526c-e64f-466e-90c9-4495b5a62a48"},"_deposit":{"id":"2003115","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2003115"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02003115","sets":["1642837622505:1642837905044:1642837912269","1642838403551:1642838407312"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"サキシマハブ毒のアルギニンエステル水解酵素に関する研究(農芸化学科)","subitem_1551255648112":"ja"},{"subitem_1551255647225":"Studies on arginine ester hydrolyzing enzyme of Sakishima habu (T. elegans) venom (Department of Agricultural Chemistry)","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"知念, 功","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"大城, ゆみ子","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"饒平名, とく子","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"小渡, 恵子","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"兼村, 憲勝","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"岩永, 貞昭","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Chinen, Isao","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Oshiro, Yumiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nohina, Tokuko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Odo, Keiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kanemura, Norikatsu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Iwanaga, Sadaaki","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"サキシマハブ毒のタンパク質水解活性を調べた結果, Tos-Arg-OMeHCl, Casein, Insoluble Collagene, Tos-Lys-OMeHCl, Ac-L-Gly-Lys-OMe AcOHに対する水解活性が見られた。その内でもTAME活性は, 49.41(x10^<-3>M TAME/mg/min)で高い値であった。このTAME水解酵素は, pH2.5&acd;11の範で安定であったが, 70℃以上(10分間)に加熱するとその活性は, 急激に減少した。またその酵素の至適温度は, 45℃であった。この蛇毒をセファデックスG-75カラムにのせ精製すると2個の活性ピークが見られ, その内の主要活性ピーク部をさらにセファデックスG-75,DEAEセルロースカラムを用いて精製すると, 最終的にこの分画から3個の活性ピークが見られた。","subitem_description_type":"Other"},{"subitem_description":"Sakishima habu venom hydrolyzes Tos-Arg-OMeHCl (49.41 TAME×10^<-3>M/mg/min), casein (13.26 tyrosine μg/mg/min), insoluble collagen (3.26 hypro. μg/mg/min), Tos-Lys-OMeHCl (1.43 TLME×10^<-3>M/mg/min), and Ac-L-Gly-Lys-OMeOH (0.38 AGLE×10^<-3>M/mg/min). The TAME hydrolyzing enzyme is stable in a pH region of 2.5 to 11 and at temperature below 50℃ (10 min); however it becomes unstable over 70℃. The enzyme activity was maxium at 45℃. Sephadex G-75 gel filtration of the venom was found to contain two active peaks, and further purification of the main active fraction by Sephadex G-75 and DEAE cellulose column chromatography was demonstrated to have three active peaks.","subitem_description_type":"Other"},{"subitem_description":"紀要論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"琉球大学農学部"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"jpn"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/4086"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0370-4246"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN00250548"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"琉球大学農学部学術報告"},{"subitem_1522650068558":"en","subitem_1522650091861":"The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1980-11-29","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"27","bibliographicPageEnd":"68","bibliographicPageStart":"57"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"KJ00000162044.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2003115/files/KJ00000162044.pdf"},"version_id":"3b0cec5e-7964-4525-9257-a884ba635389"}]},"item_title":"サキシマハブ毒のアルギニンエステル水解酵素に関する研究(農芸化学科)","item_type_id":"15","owner":"1","path":["1642837912269","1642838407312"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-02-14"},"publish_date":"2008-02-14","publish_status":"0","recid":"2003115","relation_version_is_last":true,"title":["サキシマハブ毒のアルギニンエステル水解酵素に関する研究(農芸化学科)"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T01:15:45.466753+00:00"}