{"created":"2022-01-27T02:49:00.625836+00:00","id":2003118,"links":{},"metadata":{"_buckets":{"deposit":"22222c36-a0d5-4232-aaf5-d1bfae1a53c3"},"_deposit":{"id":"2003118","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2003118"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02003118","sets":["1642837622505:1642837905044:1642837912269","1642838403551:1642838407312"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"\u6ce1\u76db\u9eb9\u83cc\u306e L-\u30a2\u30e9\u30cb\u30f3 : \u03b1-\u30b1\u30c8\u30b0\u30eb\u30bf\u30eb\u9178\u30c8\u30e9\u30f3\u30b9\u30a2\u30df\u30ca\u30fc\u30bc\u306e\u7cbe\u88fd\u3068\u6027\u8cea(\u8fb2\u82b8\u5316\u5b66\u79d1)","subitem_1551255648112":"ja"},{"subitem_1551255647225":"L-Alanine : \u03b1-ketoglutarate transaminase from Aspergillus awamori; purification and properties (Department of Agricultural Chemistry)","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"\u77f3\u539f, \u660c\u4fe1","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"\u4e0e\u90a3\u8987, \u548c\u96c4","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"\u5f53\u5c71, \u6e05\u5584","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Ishihara, Masanobu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yonaha, Kazuo","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Toyama, Seizen","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"\u5404\u7a2e\u30ab\u30d3\u306b\u304a\u3051\u308bL-\u30a2\u30e9\u30cb\u30f3 : \u03b1-\u30b1\u30c8\u30b0\u30eb\u30bf\u30eb\u9178\u30c8\u30e9\u30f3\u30b9\u30a2\u30df\u30ca\u30fc\u30bc\u6d3b\u6027\u3092\u8abf\u3079\u305f\u7d50\u679c, \u6ce1\u76db\u9eb9\u83cc(Asp. awamori IFO4122)\u3092\u8517\u7cd6-\u30da\u30d7\u30c8\u30f3\u542b\u6709\u57f9\u5730\u3067\u632f\u3068\u3046\u57f9\u990a\u3057, \u5f97\u3089\u308c\u305f\u83cc\u4f53\u306e\u7121\u7d30\u80de\u62bd\u51fa\u6db2\u4e2d\u306b\u9ad8\u3044\u9175\u7d20\u6d3b\u6027\u304c\u5f97\u3089\u308c\u305f\u3002\u672c\u9175\u7d20\u306fL-\u30a2\u30e9\u30cb\u30f3\u3068\u03b1-\u30b1\u30c8\u30b0\u30eb\u30bf\u30eb\u9178\u304b\u3089L-\u30b0\u30eb\u30bf\u30df\u30f3\u9178\u3068\u30d4\u30eb\u30d3\u30f3\u9178\u3092\u751f\u6210\u3059\u308b\u30a2\u30df\u30ce\u57fa\u8ee2\u79fb\u53cd\u5fdc\u3092\u89e6\u5a92\u3057, \u672c\u53cd\u5fdc\u306e\u9006\u53cd\u5fdc\u3082\u89e6\u5a92\u3057\u305f\u3002\u672c\u9175\u7d20\u306f, \u4f9b\u8a66\u6ce1\u76db\u9eb9\u83cc\u682a\u306e\u7121\u7d30\u80de\u62bd\u51fa\u6db2\u304b\u3089\u786b\u5b89\u5206\u753b\u53ca\u3073\u5404\u7a2e\u306e\u30ab\u30e9\u30e0\u30af\u30ed\u30de\u30c8\u30b0\u30e9\u30d5\u30a3\u30fc\u306b\u3088\u308a55\u500d\u306b\u7cbe\u88fd\u3055\u308c\u305f\u3002\u7cbe\u88fd\u9175\u7d20\u306e\u30b2\u30eb\u6eec\u904e\u6cd5\u3067\u6c42\u3081\u305f\u5206\u5b50\u91cf\u306f104,000\u3067\u3042\u308a, \u5438\u53ce\u30b9\u30da\u30af\u30c8\u30eb\u306f280nm, 320nm\u53ca\u3073418nm\u306b\u5438\u53ce\u6975\u5927\u304c\u3042\u3063\u305f, \u7cbe\u88fd\u9175\u7d20\u306fL-\u30a2\u30e9\u30cb\u30f3\u306b\u7279\u7570\u7684\u3067, L-\u30a2\u30e9\u30cb\u30f3\u53ca\u3073\u03b1-\u30b1\u30c8\u30b0\u30eb\u30bf\u30eb\u9178\u306b\u5bfe\u3059\u308bkm\u5024\u306f2.9mM\u3067\u3042\u3063\u305f\u3002\u9175\u7d20\u53cd\u5fdc\u306e\u81f3\u9069pH\u306f7.0∿8.0\u3067, \u81f3\u9069\u6e29\u5ea6\u306f45\u2103\u3067\u3042\u3063\u305f\u3002","subitem_description_type":"Other"},{"subitem_description":"The activity of L-alanine : \u03b1-ketoglutarate transaminase in the cell-free extracts of various molds has been determined. A high activity of the transaminase was found in the cell-free extract of Aspergillus awamori IFO 4122 grown on sucrose-peptone medium with shaking, and the strain was chosen for characterization and purification of the enzyme. The enzyme catalyzed reversibly the transamination between L-alanine and \u03b1-ketoglutarate producing L-glutamate and pyruvate. The enzyme was purified 55 fold from the cell-free extract, using ammonium sulfate fractionation and various chromatographic techniques. A molecular weight of 104,000 was obtained by gel filtration. The purified enzyme exhibits absorption maxima at 280nm, 320nm and 418nm, at pH 8.0. The purified enzyme was highly specific for L-alanine and the Km value for L-alanine and \u03b1-ketoglutarate was estimated to be 2.9mM. The optimal pH and temperature for the transaminase reaction were 7.0-8.0 and 45\u2103, respectively.","subitem_description_type":"Other"},{"subitem_description":"\u7d00\u8981\u8ad6\u6587","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"jpn"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/4089"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0370-4246"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN00250548"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"},{"subitem_1522650068558":"en","subitem_1522650091861":"The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1980-11-29","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"27","bibliographicPageEnd":"102","bibliographicPageStart":"89"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"KJ00000162047.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2003118/files/KJ00000162047.pdf"},"version_id":"23fadff4-4ada-4bd9-94ea-18b9fb1f4fe5"}]},"item_title":"\u6ce1\u76db\u9eb9\u83cc\u306e L-\u30a2\u30e9\u30cb\u30f3 : \u03b1-\u30b1\u30c8\u30b0\u30eb\u30bf\u30eb\u9178\u30c8\u30e9\u30f3\u30b9\u30a2\u30df\u30ca\u30fc\u30bc\u306e\u7cbe\u88fd\u3068\u6027\u8cea(\u8fb2\u82b8\u5316\u5b66\u79d1)","item_type_id":"15","owner":"1","path":["1642837912269","1642838407312"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-02-14"},"publish_date":"2008-02-14","publish_status":"0","recid":"2003118","relation_version_is_last":true,"title":["\u6ce1\u76db\u9eb9\u83cc\u306e L-\u30a2\u30e9\u30cb\u30f3 : \u03b1-\u30b1\u30c8\u30b0\u30eb\u30bf\u30eb\u9178\u30c8\u30e9\u30f3\u30b9\u30a2\u30df\u30ca\u30fc\u30bc\u306e\u7cbe\u88fd\u3068\u6027\u8cea(\u8fb2\u82b8\u5316\u5b66\u79d1)"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T01:15:49.328217+00:00"}