@article{oai:u-ryukyu.repo.nii.ac.jp:02003121, author = {安田, 正昭 and 宮里, 興信 and 島袋, 政朋 and Yasuda, Masaaki and Miyazato, Koshin and Shimabukuro, Masatomo}, issue = {27}, journal = {琉球大学農学部学術報告, The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus}, month = {Nov}, note = {放線菌のヘミセルラーゼ活性を調べた結果, さとうきび畑土壌から分離されたStreptomyces sp.No.131.菌株が最も高い活性を有することがわかった。Streptomyces sp.No.131菌株を甘蔗バガスのヘミセルロース(2%)を含むペプトン培地(初発pH8.5), 30℃, 72hr振とう培養を行うことによりヘミセルラーゼがよく生産された。本酵素は培地中の甘蔗バガスヘミセルロースにより誘導生成された。本菌の生産するヘミセルラーゼは, 甘蔗バガスヘミセルロースを加水分解しキシロオリゴ糖とキシロースを生成する酵素で, 液化型キシラナーゼである。本酵素の反応最適pHは7.5で, 反応最適温度は50℃であった。, The association constants and molar extinction coefficients of the product of the addition reaction of all-trans-retinal and 20 amino acids were determined. Those having the higher pK values of amino group, such as L-ornithine, L-lysine, and β-alanine, gave greater association constants, indicating not only the association constant of retinylidene-compound formation but also the pK value of the amino group involved in the reaction would be utilized as an indicator for the purpose of screening amino-acid related, food-originated insect-vision inhibitors at a molecular level. For aniline and hydroxylamine, the association constants and molar extinction coefficients of the respective product formed were also determined. L-Lysine, aniline, and hydroxylamine modified the photopotentials generated across all-trans-retinal bilayer lipid membranes in appropriate conditions, showing the applicability of the membrane system for screening the insect-vision inhibitors having amino groups., 紀要論文}, pages = {119--128}, title = {土壌から分離された放線菌による甘蔗バガスヘミセルロースの分解について(農芸化学科)}, year = {1980} }