{"created":"2022-01-27T07:27:23.095315+00:00","id":2003211,"links":{},"metadata":{"_buckets":{"deposit":"569ac51a-eea3-4578-83a7-068a6651fdd4"},"_deposit":{"id":"2003211","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2003211"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02003211","sets":["1642837622505:1642837905044:1642837910985","1642838403551:1642838407312"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"サキシマハブ毒のプロテアーゼに関する研究(農芸化学科)","subitem_1551255648112":"ja"},{"subitem_1551255647225":"Studies on proteases of T. elegans venom (Department of Agricultural Chemistry)","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"知念, 功","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"真栄田, 義昭","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"保良, 和政","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"四方, 治五郎","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Chinen, Isao","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Maeda, Yoshiaki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yasura, Kazumasa","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Yomo, Harugoro","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"サキシマハブ毒のプロテアーゼ活性は, カゼイン消化法で測定した場合, 至適温度は, 50℃であり, 至適pHは, 8.0&acd;9.5であった。またこの酵素はpH5&acd;10.5で安定であった。本酵素は, エタノール, メタノール, イソプロパノール, アセトン等の有機溶媒に対しては, 不安定であったが, 硫安に対しては, 安定であるが, 長時間放置すると失活した。また塩化カルシウム, 塩化マグネシウム, 塩化亜鉛で賦活効果が見られ, 特にCaCl_2では, その効果が大であった。本酵素をセファデックスG-75,G-100,G-50,およびDEAEセルロースカラムの順にのせて行き精製した結果, 本酵素は, ほぼ単一になり, その純化率は, 3.4培になり, タンパク質回収率は, 4.1%であった。またこの精製酵素は, Ca^<2+>を有していた。その他に本蛇毒中には, Zn^<2+>を有するプロテアーゼが存在すると推定された。","subitem_description_type":"Other"},{"subitem_description":"The proteinase of T. elegans venom was most active at pH8.0-9.5 and 50℃ with 1% casein solution as substrate, stable between pH5.0 and 10.5 and by addition of ammonium sulfate, but unstable by addition of organic solvents; ethanol, methanol, isopropanol, and acetone. The enzyme was inactivated by addition of EDTA and reactivated by CaCl_2 and MgCl_2. A proteinase was purified from the venom by gel filtration on Sephadex G-75,G-100,and G-50,and DEAE cellulose column chromatography with 4.1% over all yield and a 3.4 fold increase in specific activity. The purified enzyme was found to be homogeneous by disc gel electrophoresis and contain (Ca)^<++>. There was the orther proteinase in this venom, which contained (Zn)^<++>.","subitem_description_type":"Other"},{"subitem_description":"紀要論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"琉球大学農学部"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"jpn"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/4197"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0370-4246"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN00250548"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"琉球大学農学部学術報告"},{"subitem_1522650068558":"en","subitem_1522650091861":"The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1978-12-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"25","bibliographicPageEnd":"216","bibliographicPageStart":"203"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"KJ00000161951.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2003211/files/KJ00000161951.pdf"},"version_id":"0b8287ee-304a-465e-ad54-3fdbbbfe852d"}]},"item_title":"サキシマハブ毒のプロテアーゼに関する研究(農芸化学科)","item_type_id":"15","owner":"1","path":["1642837910985","1642838407312"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-02-14"},"publish_date":"2008-02-14","publish_status":"0","recid":"2003211","relation_version_is_last":true,"title":["サキシマハブ毒のプロテアーゼに関する研究(農芸化学科)"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T01:17:44.043360+00:00"}