{"created":"2022-01-27T07:30:25.530180+00:00","id":2003313,"links":{},"metadata":{"_buckets":{"deposit":"3236c701-5338-43c3-86cb-8dcb00293a6d"},"_deposit":{"id":"2003313","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2003313"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02003313","sets":["1642837622505:1642837905044:1642837909579","1642838403551:1642838407312"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"イネにおける細胞質雄性不稔と稔性回復の遺伝学的研究(農学部)","subitem_1551255648112":"ja"},{"subitem_1551255647225":"Genetical Studies of Cytoplasmic Male Sterility and Fertility Restoration in Rice, Oryza sativa L. (Department of Agriculture)","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"新城, 長有","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Shinjyo, Choyu","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"筆者はインド型イネChinsurah Boro II品種が雄性不稔細胞質と稔性回復遺伝子をもつことを発見し, 連続戻交雑法を用いて両因子を日本型イネの台中65号へ導入した。育成された上記isogenic系統を主材料にして, 雄性不稔の遺伝, 他形質に対する細胞質と稔性回復遺伝子の効果, 稔性回復遺伝子の座位, 雄性不稔イネにおける花粉退化の時期, 雄性不稔細胞質と稔性回復遺伝子の地理的分布, および雑種イネ育種に必要な三系統の育成法を研究した。結論の要約は下記のとおりである。A雄性不稔の遺伝インド型イネChinsurah BoroIIに由来する雄性不稔細胞質を(ms-boro), その稔性回復遺伝子をRfと命名した。(ms-boro)Rf Rfの遺伝子型をもつ系統は完全雄性稔, (ms-boro)Rf rfは部分雄性稔(花粉稔性約50%)で, (ms-boro)rf rfは完全雄性不稔であった。一方上記3系統の雌性配偶子は健全であった。正常細胞質(n-boro)をもつ個体は核内遺伝子型に関係なく, すべて完全雄性稔になった。(ms-boro)rf rf×(n-boro)Rf rfのF_1世代においては, 部分雄性稔と完全雄性不稔個体が1 : 1の比に分離したが, (ms-boro)rf rf×(ms-boro)Rf rfのF_1では稔性の分離は観察されず, すべての個体が部分雄性稔(花粉稔性約50%)になった。(ms-boro)Rf rf系統の自殖次代には完全雄性稔および部分雄性稔個体が1 : 1の比に分離した。したがって(ms-boro)Rf rf個体においては, 花粉形成期のある時期にrf遺伝子をもつ花粉は雄性不稔細胞質との相互作用で死滅し, Rf花粉のみが正常に発育するといういわゆる雄性配偶体不稔性と結論した。(ms-boro)rf rf×Rf RfのF_1個体の花分稔性は50%を示すが, 種子稔性は90%以上になる。したがって本雄性不稔細胞質と稔性回復遺伝子は雑種イネの育成に利用できるものと考えられる。なお, 雑種イネ育成に必要な3系統, すなわち雄性不稔系統, 雄性不稔維持系統および稔性回復系統の育成方法についても理論的に示した。B量的形質に対する細胞質と核内遺伝子の効果作出可能な6 isogenic系統を育成し, 5反覆の乱魂法を用いて, 1970年の第1期作と第2期作で栽培し, 出穂日, 穂数, 主稈葉数, 稈長, 第1節間長, 第2節間長および第3節間長(節間は最上位から数えた。)を測定し, 系統間の比較を行った。","subitem_description_type":"Other"},{"subitem_description":"雄性不稔系統の稈長は他の5系統に比較して約7cm短く, 1%水準で有意であった。雄性不稔系統の短稈性は, おもに第1∿第3節間長の短縮に起因する。他の5系統の稈長間には有意差はなかった。雄性不稔系統の示す出穂日, 穂数, 主稈葉数は他の系統と同程度であった。雄性不稔系統のこのような特性は交雑圃における受粉体制に好影響をもたらすものと考えられる。C Rf遺伝子の座位まず三染色体系統を用いて, Rf遺伝子の座乗染色体を確定し, つぎに既2標識遺伝子系統との交雑を行ない座位を明らかにした。三染色体分析では3系交雑法を適用した。まずTrisomics×(ms-boro)Rf Rfの交雑F_1から三染体個体を染色体数の観察によって迸抜し, つぎにF_1の三染色体植物を父本にして雄性不稔系統へ交雑し, 次代植物の種子稔性を調査した。その結果Rf遺伝子はTrisomic C系統の過剰染色体, つまり岩田らの第7染色体に座上することが判明した。Rf fl間の組換価は約0.4%で, pglとRf間のそれは約12%, pglとfl間は約20%であった。したがって第7染色体上における遺伝子の配列順序はpgl-Rf-flである。D花粉退化の細胞組識学的研究本雄性不稔系統における花粉の発育過程を観察した。滅数分列は正常に進行し, 花分四分子も正常に形成される。しかし花粉1核期でその発育を停止し, 2核期以後は観察されない。タペート細胞は正常である。出穂期の不稔花粉はヨード・ヨードカリ液で染色されない。不稔花粉は球形で発芽孔を有するが, 形は正常花粉よりも小さい。E雄性不稔細胞質と稔性回復遺伝子の分布(1)日本の水稲奨励品種について細胞質と核内遺伝子型の検定法を考案し, その検定法に基づいて1962年度の日本水稲奨励品種150品種を検定した。19品種(12.7%)の品種は弱稔性回復遺伝子をもち, 他の131品種は非回復遺伝子をもっていた。弱回復遺伝子をもつ品種のほとんどは京都以南に集中的に分布した。これらの品種のほとんどは在来品種から分離育種法によって育成された品種であった。供試日本稲品種には雄性不稔細胞質は発見されなかった。(2)外国品種について本研究に用いたイネ品種は15国から蒐集した153品種でった。細胞質検定親に遺伝子型(n-boro)rf rfの6系統を, 核内遺伝子検定親には遺伝子型(ms-boro)rf rfの6系統を用いた。これらの検定親と153品種との交雑を行なった。主としてF_1の花粉および種子稔性から, それぞれの品種の細胞質型と核内遺伝子型を推定した。雑種不稔性の併発によって, これらの推定が困難であった組合せについては, B_1F_1か自家受粉による後代系統の稔性から推定した。","subitem_description_type":"Other"},{"subitem_description":"This research work was carried out to elucidate five genetical problems on the cytoplasmic male sterility and fertility restoration, and to probe the possibility of future hybrid rice breeding in the sophisticated way by making use of the cytoplasmic male sterility in this important cereal crop. The conclusions arrived at are summarized in the following paragraphs. A. Inheritance of male sterility A case of cytoplasmically male sterility controlled by a fertility-restoring gene Rf was found by the present author in a cultivated rice, Oryza sativa L. The male sterility-inducing cytoplasm symbolized by (ms-boro) and fertility-restoring gene Rf were derived from an Indica variety, Chinsurah Boro II, and the experiments were made by the isogenic lines having the same genetic background of a Japonica variety, Taichung 65. When a plant with male sterility-inducing cytoplasm (ms-boro) had nuclear genotype Rf Rf, it was completely male-fertile; when it had Rf rf, partially male-fertile (ca. 50 percent); and when it had rf rf, completely male-sterile with no restiction in ovule fertility. A plant with normal cytoplasm (n-boro) would be completely male-fertile regardless of the nuclear genotype for fertility-restoring genes. The F_1 plants of (ms-boro) rf rf x (n-boro) Rf rf segregated into partially male-fertile and completely male-sterile classes in a ratio of 1 : 1,while the F_1 plants of (ms-boro) rf rf x (ms-boro) Rf rf did not segregate, and all of the plants revealed only partially male fertility. The selfed progeny of (ms-boro) Rf rf line segregated into completely male-fertile and partially male-fertile classes in a ratio of 1 : 1. The effect of fertility-restoring gene is thus found to be gametophytic type in the male sterility-inducing cytoplasm.","subitem_description_type":"Other"},{"subitem_description":"The F_1 plants of (ms-boro) rf rf x Rf Rf have revealed a 90 percent or higher spikelet fertility, though they are partially male-fertile. The sources of cytoplasm and fertility-restoring gene may be used for breeding \"hybrid rice\". It is clear that the developments of three lines (male-sterile, maintainer and restorer lines) needing for hybrid rice breeding are possible by using methods of Figs. 8,9 or 10. B. Effects of cytoplasm and nuclear genotype for fertility-restoration on main quantitative characters Seven quantitative characters, namely, heading date, ear number, leaf number of main culm, culm length, and each of three internodes (first, second and third) counted from the uppermost internode to lower, of six isogenic lines were studied. About 7cm shorter culm and its related three shorter internodes were appeared alone on the male sterile line, but were not related in those of five other isogenic lines. Nevertheless, the male sterile line had the shorter culm including three shorter internodes, the leaf number (or node number), heading date, and ear number per plant of the male sterile line, were the same degree as those of the other lines. These are the most desirable factors for cross pollinated system in the production of hybrid seed in commercial scale. C. Linkage analysis for fertility-restoring gene Rf Two analyzing methods were employed. Firstly, each of 12 F_1 trisomic plants derived from 12 trisomic lines x strain (ms-boro) Rf-Taichung 65 was selected cytologically, and the selected F_1 trisomic plants were crossed again as paternal parent to (ms-boro) rf-Taichung 65 (male sterile line). From the results of spikelet fertility of the F_1 progenies derived from above three way cross, it is clear that the fertility restoring gene is carried by the extra chromosome of trisomic C line which is to be the seventh chromosome assumed by Iwata and Omura (35). In the second, the recombination values among three genes, fl, pgl, and Rf, which were carried by the same chromosome were calculated by conventional methods used first backcross or F_2 progenies. The values between fl and Rf is about 0.4 percent, pgl and Rf is about 12 percent, and fl and pgl is about 20 percent.","subitem_description_type":"Other"},{"subitem_description":"紀要論文","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"琉球大学農学部"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/4321"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"ISSN","subitem_1522646572813":"0370-4246"},{"subitem_1522646500366":"NCID","subitem_1522646572813":"AN00250548"}]},"item_1617186941041":{"attribute_name":"Source Title","attribute_value_mlt":[{"subitem_1522650068558":"ja","subitem_1522650091861":"琉球大学農学部学術報告"},{"subitem_1522650068558":"en","subitem_1522650091861":"The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1975-12-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"22","bibliographicPageEnd":"57","bibliographicPageStart":"1"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"KJ00000162506.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2003313/files/KJ00000162506.pdf"},"version_id":"310ba239-e025-412b-ae35-80ad4451e10a"}]},"item_title":"イネにおける細胞質雄性不稔と稔性回復の遺伝学的研究(農学部)","item_type_id":"15","owner":"1","path":["1642837909579","1642838407312"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2008-02-14"},"publish_date":"2008-02-14","publish_status":"0","recid":"2003313","relation_version_is_last":true,"title":["イネにおける細胞質雄性不稔と稔性回復の遺伝学的研究(農学部)"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T01:19:45.927353+00:00"}