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  1. 紀要論文
  2. 琉球大学農学部学術報告
  3. 20号
  1. 部局別インデックス
  2. 農学部

細菌の細胞懸濁液によるタウリンーピルビン酸アミノ基転移反応について(農芸化学科)

http://hdl.handle.net/20.500.12000/4426
http://hdl.handle.net/20.500.12000/4426
429d616d-eccf-49c1-9772-b84c80cee4c1
名前 / ファイル ライセンス アクション
KJ00000162435.pdf KJ00000162435.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2008-02-14
タイトル
タイトル 細菌の細胞懸濁液によるタウリンーピルビン酸アミノ基転移反応について(農芸化学科)
言語 ja
作成者 当山, 清善

× 当山, 清善

ja 当山, 清善

宮里, 興信

× 宮里, 興信

ja 宮里, 興信

当間, 孝子

× 当間, 孝子

ja 当間, 孝子

左右田, 健次

× 左右田, 健次

ja 左右田, 健次

Toyama, Seizen

× Toyama, Seizen

en Toyama, Seizen

Miyazato, Koshin

× Miyazato, Koshin

en Miyazato, Koshin

Toma, Takako

× Toma, Takako

en Toma, Takako

Soda, Kenji

× Soda, Kenji

en Soda, Kenji

アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
内容記述
内容記述タイプ Other
内容記述 土壌などの試料から分離した細菌, W-64菌株の菌体懸濁液を酵素液としてタウリンーピルビン酸アミノ基転移反応を触媒する酵素について調べ, 次の結果を得た。1.菌体のタウリンーピルビン酸アミノ基転移反応活性は培地にβ-アラニンを加えることによって著しく増大した。2.酵素反応におけるα-アラニンの生成にはタウリンおよびピルビン酸の両基質が必要であった。生成するα-アラニンの量は反応時間および酵素量に比例した。アミノ基受容体としてはピルビン酸が良好な基質であった。反応の最適pHは8.0付近にあった。3.β-アラニン含有培地で得た菌体はピルビン酸をアミノ基受容体としてβ-アラニン, γ-アミノ酪酸およびε-アミノカプロン酸をアミノ基供与体とするアミノ基転移反応も触媒した。
内容記述タイプ Other
内容記述 This paper presents the enzyme catalyzing the transaminatition of taurine with pyruvate in the cell suspension of W-64 strain, a bacterium which utilizes taurine as a sole carbon and nitrogen source. 1. The transaminase activity of the cells was enhanced greatly by addition of β-alanine to the growth medium. 2. In the enzyme reaction, both taurine and pyruvate as substrate were required to produce α-alanine. The formation of α-alanine proceeded linearly as a function of incubation time and cell concentration. Pyruvate was one of the best amino acceptor. The pH optimum of the reaction was around 8.0. 3. β-Alanine-grown cells also catalyzed the transamination reaction of γ-aminobutyrate, β-alanine, ε-aminocaproate, and taurine in this order with pyruvate.
内容記述タイプ Other
内容記述 紀要論文
出版者
言語 ja
出版者 琉球大学農学部
言語
言語 jpn
資源タイプ
資源タイプ departmental bulletin paper
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
識別子
識別子 http://hdl.handle.net/20.500.12000/4426
識別子タイプ HDL
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0370-4246
収録物識別子タイプ NCID
収録物識別子 AN00250548
収録物名
言語 ja
収録物名 琉球大学農学部学術報告
書誌情報
号 20, p. 105-113
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