{"_buckets": {"deposit": "dbfd9654-83db-4b1c-b6da-3a072fc5f23d"}, "_deposit": {"id": "2003394", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2003394"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02003394", "sets": ["1642837908633", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "細菌の細胞懸濁液によるタウリンーピルビン酸アミノ基転移反応について(農芸化学科)", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "On the transamination reaction of taurine with pyruvate in the cell suspension of a soil bacterium (Department of Agricultural Chemistry)", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "当山, 清善", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "宮里, 興信", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "当間, 孝子", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "左右田, 健次", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Toyama, Seizen", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Miyazato, Koshin", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Toma, Takako", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Soda, Kenji", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "土壌などの試料から分離した細菌, W-64菌株の菌体懸濁液を酵素液としてタウリンーピルビン酸アミノ基転移反応を触媒する酵素について調べ, 次の結果を得た。1.菌体のタウリンーピルビン酸アミノ基転移反応活性は培地にβ-アラニンを加えることによって著しく増大した。2.酵素反応におけるα-アラニンの生成にはタウリンおよびピルビン酸の両基質が必要であった。生成するα-アラニンの量は反応時間および酵素量に比例した。アミノ基受容体としてはピルビン酸が良好な基質であった。反応の最適pHは8.0付近にあった。3.β-アラニン含有培地で得た菌体はピルビン酸をアミノ基受容体としてβ-アラニン, γ-アミノ酪酸およびε-アミノカプロン酸をアミノ基供与体とするアミノ基転移反応も触媒した。", "subitem_description_type": "Other"}, {"subitem_description": "This paper presents the enzyme catalyzing the transaminatition of taurine with pyruvate in the cell suspension of W-64 strain, a bacterium which utilizes taurine as a sole carbon and nitrogen source. 1. The transaminase activity of the cells was enhanced greatly by addition of β-alanine to the growth medium. 2. In the enzyme reaction, both taurine and pyruvate as substrate were required to produce α-alanine. The formation of α-alanine proceeded linearly as a function of incubation time and cell concentration. Pyruvate was one of the best amino acceptor. The pH optimum of the reaction was around 8.0. 3. β-Alanine-grown cells also catalyzed the transamination reaction of γ-aminobutyrate, β-alanine, ε-aminocaproate, and taurine in this order with pyruvate.", "subitem_description_type": "Other"}, {"subitem_description": "紀要論文", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "琉球大学農学部"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/4426"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "琉球大学農学部学術報告"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1973-12-01", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "20", "bibliographicPageEnd": "113", "bibliographicPageStart": "105"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00000162435.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2003394/files/KJ00000162435.pdf"}, "version_id": "bd63381d-b55b-4ccf-8404-bf73883dab6b"}]}, "item_title": "細菌の細胞懸濁液によるタウリンーピルビン酸アミノ基転移反応について(農芸化学科)", "item_type_id": "15", "owner": "1", "path": ["1642837908633", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/4426", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2003394", "relation": {}, "relation_version_is_last": true, "title": ["細菌の細胞懸濁液によるタウリンーピルビン酸アミノ基転移反応について(農芸化学科)"], "weko_shared_id": -1}