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  1. 紀要論文
  2. 琉球大学農学部学術報告
  3. 19号
  1. 部局別インデックス
  2. 農学部

細胞融合によるイヌ伝染性肝炎ウイルス誘発ハムスター腫瘍細胞からイヌ細胞への T 抗原の移行(畜産学科)

http://hdl.handle.net/20.500.12000/4473
http://hdl.handle.net/20.500.12000/4473
ccfe3e63-acd5-4250-86a1-fa824f9b4401
名前 / ファイル ライセンス アクション
KJ00000162415.pdf KJ00000162415.pdf
Item type デフォルトアイテムタイプ(フル)(1)
公開日 2008-02-14
タイトル
タイトル 細胞融合によるイヌ伝染性肝炎ウイルス誘発ハムスター腫瘍細胞からイヌ細胞への T 抗原の移行(畜産学科)
言語 ja
タイトル
タイトル Transfer of T antigen from ICH virus-induced hamster tumor cells to dog kidney cells by cell fusion technique (Department of Animal Husbandry)
言語 en
作成者 金城, 俊夫

× 金城, 俊夫

ja 金城, 俊夫

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西, 武

× 西, 武

ja 西, 武

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梁川, 良

× 梁川, 良

ja 梁川, 良

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Kinjo, Toshio

× Kinjo, Toshio

en Kinjo, Toshio

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Nishi, Takeshi

× Nishi, Takeshi

en Nishi, Takeshi

Search repository
Yanagawa, Ryo

× Yanagawa, Ryo

en Yanagawa, Ryo

Search repository
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
内容記述
内容記述タイプ Other
内容記述 ICHウイルス誘発ハムスター腫瘍よりin vitroで確立したHT-7細胞とイヌ腎由来の株化細胞MDCKをUV不活化HVJの仲介のもとに細胞融合を起させ, HT-7細胞の有するT抗原をMDCKに移行させるべく, 実験を試みた。その結果, 細胞融合により約10∿30%のホモあるいはヘテロカリオンが形成されたが, 多核体のヘテロカリオン中に螢光抗体法によってT抗原を有するMDCKの核がみられ, イヌ細胞へのT抗原の移行を確認できた。なお, 細胞融合によってICHウイルスの活性化はできなかったが, 一部のMDCKの核に, ICHウイルス増殖の過程でみられる核内封入体と類似のものが観察され, このものはT抗体によって染色された。融合細胞を7∿10代継代後, その100∿500万個を生後96時間以内の仔イヌの皮下に移植を行ない, 腫瘍の発現の可能性を検討した。その結果, 細胞移植後1∿2週の間に局所に小指頭大∿拇指頭大の腫瘤の発生があり, その大きさはHVJ仲介のもとで融合させた細胞を移植されたイヌで著明であった。しかし, 腫瘤の病理組織学的観察結果は, 移植細胞の増殖によるものでなく, それをとりまく生体側の肉芽腫形成と診断され, 細胞融合法によってT抗原産生能を獲得したと思われるMDCKによる腫瘍の発現は確認し得なかった。
内容記述
内容記述タイプ Other
内容記述 Attempts to transfer T antigen from HT-7 cells originated from ICH virusinduced hamster tumor to established dog kidney cells (MDCK) were carried out using cell fusion technique. The percentage of multinucleated homo- and hetero-karyons produced after fusion of the both cell types in the presence of inactivated HVJ was approximately 10-30%. When heterokaryon cultures were examined for presence of T antigen by immunofluorescent staining, some multinucleated cells contained fluorescing nuclei being of MDCK origin. The fact seems to indicate that transfer of T antigen from the HT-7 cells to MDCK took place. Attempts to reactivate ICH virus from the HT-7 cells by fusion technique were unsuccessful, but in some nuclei of MDCK origin, inclusion body-like structure resembling that of ICH virus-infected MDCK was observed. The structure was shown to contain T antigen by immunofluorescent staining. To determine whether the MDCK thus treated can produce tumor in dogs, the fused cells after being passaged for 7 to 10 times were inoculated subcutaneously into baby dogs less than 96 hours of age. Within 2 weeks after inoculation, palpable mass at site of inoculation was noted in some of the dogs but was regressed gradually thereafter. On histological examination, the mass was designated granulom. Tumor development could not be confirmed by such methods.
内容記述
内容記述タイプ Other
内容記述 紀要論文
出版者
言語 ja
出版者 琉球大学農学部
言語
言語 jpn
資源タイプ
資源タイプ departmental bulletin paper
資源タイプ識別子 http://purl.org/coar/resource_type/c_6501
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
識別子
識別子 http://hdl.handle.net/20.500.12000/4473
識別子タイプ HDL
収録物識別子
収録物識別子タイプ ISSN
収録物識別子 0370-4246
収録物識別子
収録物識別子タイプ NCID
収録物識別子 AN00250548
収録物名
言語 ja
収録物名 琉球大学農学部学術報告
収録物名
言語 en
収録物名 The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus
書誌情報
号 19, p. 431-443, 発行日 1972-12-01
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