{"_buckets": {"deposit": "85c0b895-4ee3-421e-a003-b7faa91c6cba"}, "_deposit": {"id": "2003441", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2003441"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02003441", "sets": ["1642837908174", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "\u7d30\u80de\u878d\u5408\u306b\u3088\u308b\u30a4\u30cc\u4f1d\u67d3\u6027\u809d\u708e\u30a6\u30a4\u30eb\u30b9\u8a98\u767a\u30cf\u30e0\u30b9\u30bf\u30fc\u816b\u760d\u7d30\u80de\u304b\u3089\u30a4\u30cc\u7d30\u80de\u3078\u306e T \u6297\u539f\u306e\u79fb\u884c(\u755c\u7523\u5b66\u79d1)", "subitem_1551255648112": "ja"}, {"subitem_1551255647225": "Transfer of T antigen from ICH virus-induced hamster tumor cells to dog kidney cells by cell fusion technique (Department of Animal Husbandry)", "subitem_1551255648112": "en"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u91d1\u57ce, \u4fca\u592b", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u897f, \u6b66", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u6881\u5ddd, \u826f", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Kinjo, Toshio", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nishi, Takeshi", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Yanagawa, Ryo", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "ICH\u30a6\u30a4\u30eb\u30b9\u8a98\u767a\u30cf\u30e0\u30b9\u30bf\u30fc\u816b\u760d\u3088\u308ain vitro\u3067\u78ba\u7acb\u3057\u305fHT-7\u7d30\u80de\u3068\u30a4\u30cc\u814e\u7531\u6765\u306e\u682a\u5316\u7d30\u80deMDCK\u3092UV\u4e0d\u6d3b\u5316HVJ\u306e\u4ef2\u4ecb\u306e\u3082\u3068\u306b\u7d30\u80de\u878d\u5408\u3092\u8d77\u3055\u305b, HT-7\u7d30\u80de\u306e\u6709\u3059\u308bT\u6297\u539f\u3092MDCK\u306b\u79fb\u884c\u3055\u305b\u308b\u3079\u304f, \u5b9f\u9a13\u3092\u8a66\u307f\u305f\u3002\u305d\u306e\u7d50\u679c, \u7d30\u80de\u878d\u5408\u306b\u3088\u308a\u7d0410\u0026acd;30%\u306e\u30db\u30e2\u3042\u308b\u3044\u306f\u30d8\u30c6\u30ed\u30ab\u30ea\u30aa\u30f3\u304c\u5f62\u6210\u3055\u308c\u305f\u304c, \u591a\u6838\u4f53\u306e\u30d8\u30c6\u30ed\u30ab\u30ea\u30aa\u30f3\u4e2d\u306b\u87a2\u5149\u6297\u4f53\u6cd5\u306b\u3088\u3063\u3066T\u6297\u539f\u3092\u6709\u3059\u308bMDCK\u306e\u6838\u304c\u307f\u3089\u308c, \u30a4\u30cc\u7d30\u80de\u3078\u306eT\u6297\u539f\u306e\u79fb\u884c\u3092\u78ba\u8a8d\u3067\u304d\u305f\u3002\u306a\u304a, \u7d30\u80de\u878d\u5408\u306b\u3088\u3063\u3066ICH\u30a6\u30a4\u30eb\u30b9\u306e\u6d3b\u6027\u5316\u306f\u3067\u304d\u306a\u304b\u3063\u305f\u304c, \u4e00\u90e8\u306eMDCK\u306e\u6838\u306b, ICH\u30a6\u30a4\u30eb\u30b9\u5897\u6b96\u306e\u904e\u7a0b\u3067\u307f\u3089\u308c\u308b\u6838\u5185\u5c01\u5165\u4f53\u3068\u985e\u4f3c\u306e\u3082\u306e\u304c\u89b3\u5bdf\u3055\u308c, \u3053\u306e\u3082\u306e\u306fT\u6297\u4f53\u306b\u3088\u3063\u3066\u67d3\u8272\u3055\u308c\u305f\u3002\u878d\u5408\u7d30\u80de\u30927\u0026acd;10\u4ee3\u7d99\u4ee3\u5f8c, \u305d\u306e100\u0026acd;500\u4e07\u500b\u3092\u751f\u5f8c96\u6642\u9593\u4ee5\u5185\u306e\u4ed4\u30a4\u30cc\u306e\u76ae\u4e0b\u306b\u79fb\u690d\u3092\u884c\u306a\u3044, \u816b\u760d\u306e\u767a\u73fe\u306e\u53ef\u80fd\u6027\u3092\u691c\u8a0e\u3057\u305f\u3002\u305d\u306e\u7d50\u679c, \u7d30\u80de\u79fb\u690d\u5f8c1\u0026acd;2\u9031\u306e\u9593\u306b\u5c40\u6240\u306b\u5c0f\u6307\u982d\u5927\u0026acd;\u62c7\u6307\u982d\u5927\u306e\u816b\u7624\u306e\u767a\u751f\u304c\u3042\u308a, \u305d\u306e\u5927\u304d\u3055\u306fHVJ\u4ef2\u4ecb\u306e\u3082\u3068\u3067\u878d\u5408\u3055\u305b\u305f\u7d30\u80de\u3092\u79fb\u690d\u3055\u308c\u305f\u30a4\u30cc\u3067\u8457\u660e\u3067\u3042\u3063\u305f\u3002\u3057\u304b\u3057, \u816b\u7624\u306e\u75c5\u7406\u7d44\u7e54\u5b66\u7684\u89b3\u5bdf\u7d50\u679c\u306f, \u79fb\u690d\u7d30\u80de\u306e\u5897\u6b96\u306b\u3088\u308b\u3082\u306e\u3067\u306a\u304f, \u305d\u308c\u3092\u3068\u308a\u307e\u304f\u751f\u4f53\u5074\u306e\u8089\u82bd\u816b\u5f62\u6210\u3068\u8a3a\u65ad\u3055\u308c, \u7d30\u80de\u878d\u5408\u6cd5\u306b\u3088\u3063\u3066T\u6297\u539f\u7523\u751f\u80fd\u3092\u7372\u5f97\u3057\u305f\u3068\u601d\u308f\u308c\u308bMDCK\u306b\u3088\u308b\u816b\u760d\u306e\u767a\u73fe\u306f\u78ba\u8a8d\u3057\u5f97\u306a\u304b\u3063\u305f\u3002", "subitem_description_type": "Other"}, {"subitem_description": "Attempts to transfer T antigen from HT-7 cells originated from ICH virusinduced hamster tumor to established dog kidney cells (MDCK) were carried out using cell fusion technique. The percentage of multinucleated homo- and hetero-karyons produced after fusion of the both cell types in the presence of inactivated HVJ was approximately 10-30%. When heterokaryon cultures were examined for presence of T antigen by immunofluorescent staining, some multinucleated cells contained fluorescing nuclei being of MDCK origin. The fact seems to indicate that transfer of T antigen from the HT-7 cells to MDCK took place. Attempts to reactivate ICH virus from the HT-7 cells by fusion technique were unsuccessful, but in some nuclei of MDCK origin, inclusion body-like structure resembling that of ICH virus-infected MDCK was observed. The structure was shown to contain T antigen by immunofluorescent staining. To determine whether the MDCK thus treated can produce tumor in dogs, the fused cells after being passaged for 7 to 10 times were inoculated subcutaneously into baby dogs less than 96 hours of age. Within 2 weeks after inoculation, palpable mass at site of inoculation was noted in some of the dogs but was regressed gradually thereafter. On histological examination, the mass was designated granulom. Tumor development could not be confirmed by such methods.", "subitem_description_type": "Other"}, {"subitem_description": "\u7d00\u8981\u8ad6\u6587", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/4473"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "ISSN", "subitem_1522646572813": "0370-4246"}, {"subitem_1522646500366": "NCID", "subitem_1522646572813": "AN00250548"}]}, "item_1617186941041": {"attribute_name": "Source Title", "attribute_value_mlt": [{"subitem_1522650068558": "ja", "subitem_1522650091861": "\u7409\u7403\u5927\u5b66\u8fb2\u5b66\u90e8\u5b66\u8853\u5831\u544a"}, {"subitem_1522650068558": "en", "subitem_1522650091861": "The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueNumber": "19", "bibliographicPageEnd": "443", "bibliographicPageStart": "431"}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "KJ00000162415.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2003441/files/KJ00000162415.pdf"}, "version_id": "12bd4572-7b83-45b3-a90f-ed31747ec250"}]}, "item_title": "\u7d30\u80de\u878d\u5408\u306b\u3088\u308b\u30a4\u30cc\u4f1d\u67d3\u6027\u809d\u708e\u30a6\u30a4\u30eb\u30b9\u8a98\u767a\u30cf\u30e0\u30b9\u30bf\u30fc\u816b\u760d\u7d30\u80de\u304b\u3089\u30a4\u30cc\u7d30\u80de\u3078\u306e T \u6297\u539f\u306e\u79fb\u884c(\u755c\u7523\u5b66\u79d1)", "item_type_id": "15", "owner": "1", "path": ["1642837908174", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/4473", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2008-02-14"}, "publish_date": "2008-02-14", "publish_status": "0", "recid": "2003441", "relation": {}, "relation_version_is_last": true, "title": ["\u7d30\u80de\u878d\u5408\u306b\u3088\u308b\u30a4\u30cc\u4f1d\u67d3\u6027\u809d\u708e\u30a6\u30a4\u30eb\u30b9\u8a98\u767a\u30cf\u30e0\u30b9\u30bf\u30fc\u816b\u760d\u7d30\u80de\u304b\u3089\u30a4\u30cc\u7d30\u80de\u3078\u306e T \u6297\u539f\u306e\u79fb\u884c(\u755c\u7523\u5b66\u79d1)"], "weko_shared_id": -1}