{"created":"2022-01-27T08:05:38.024995+00:00","id":2004454,"links":{},"metadata":{"_buckets":{"deposit":"dc3b7aef-73c3-47a4-b26d-57106adf4960"},"_deposit":{"id":"2004454","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2004454"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02004454","sets":["1642838403123","1642838403551:1642838406414"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_title":"沖縄産海藻類の化学的研究","subitem_title_language":"ja"},{"subitem_title":"Chemical Investigation of Okinawan Marine Algae","subitem_title_language":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"国吉, 正之","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Kuniyoshi, Masayuki","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_access_right":"open access","subitem_access_right_uri":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_subject":"海洋天然物化学","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"海藻","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"TNF-α","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"COX","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"テルペン","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"NMR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"抗菌","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"抗カビ","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"Marine natural products","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Algae","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"TNF-α","subitem_subject_language":"ja","subitem_subject_scheme":"Other"},{"subitem_subject":"COX","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Terpenes","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"NMR","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Antibacterial","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Antifungal","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"科研費番号: 16550042","subitem_description_type":"Other"},{"subitem_description":"平成16年度～平成17年度 科学研究費補助金基盤研究(C) 研究成果報告書","subitem_description_type":"Other"},{"subitem_description":"研究概要 : 本年度は,主に褐藻Stypopodium zonaleの成分研究及びS.zonale, Laurencia luzonensis, L.brongniartii, Porteria hornemanniiからこれまでに単離された化合物の生理活性テストを行った.沖縄県中部の金武湾沿岸で採集されたS.zonale(9.75Kg) をエタノールに数日間浸漬した後,常法にしたがって分離・精製を行ない,各種機器スペクトル(NMR, IR, MS, UV)を測定し構造の解析を行なった.その結果,多くの既知化合物と共に,6種の新規メロジテルペンを得た.この内4種の化合物は,枯草菌 (Bacillus subtilis)に対し一定の阻害活性を示し,さらに炎症性サイトカイン(TNF-α)の働きを阻害することもわかった.また,この内の1種はヒトの胚性腎細胞(HEK-293)におけるアセチルコリンエステラーゼの働きを阻害した.L.luzonensisからは,前年度報告した新規化合物(10種) に加え,さらに5種の新規セスキテルペンを単離した.これらの化合物について生理活性試験を行ったところ,この内の2種はHEK-293におけるアセチルコリンエステラーゼの働きを阻害することがわかった.L.brongniartiiから単離された化合物の内,2種はCOX-1,COX-2に対して強い阻害活性を示した.また,この内の1種はHistamine H_2に対しても強い阻害活性を持つ事がわかった.P.hornemanniiから得られた3種の新規ハロゲン化モノテルペン及びそのアセチル化誘導体について活性試験を行った結果,この内の2種のアセチル化誘導体がAlternalia sp.に対して強い抗カビ活性を示した.また,1種の新規化合物と3種の既知化合物がブラインシュリンプ(Artemia salina)毒性試験において強い活性を示した.","subitem_description_type":"Other"},{"subitem_description":"In this year, we have done chemical investigation of Stypopodium zonale and some bioactivity tests of compounds from S.zonale, Laurencia luzonensis, L.brongniartii, Porteria hornemanni. S.zonale collected at Kin bay was extracted with EtOH, and then the extract was separated using various chromatographic techniques. Six new meroditerpenes were isolated together with other known compounds. Structures of these new compounds were elucidated using NMR spectroscopy and ESIMS. Four of them have shown fairly good antibacterial activity against Bacillus subtilis and inhibition of TNF-α. Also one of them has displayed the inhibition of Acetylcholine esterase (HEK-293). From the L.luzonensis, five new sesquiterpenes have been isolated including ten compounds that were reported last year. Two of them inhibit the functions of COX-1 and COX-2 while one of them inhibits the function of Histamine H_2. Three acetylated compounds from P.hornemanni have shown relatively good antifungal activity against Alternalia sp.. One new compound and three known compounds have displayed potent activity on the brineshrimp lethality test.","subitem_description_type":"Other"},{"subitem_description":"未公開：P.39～48（論文別刷のため）","subitem_description_type":"Other"},{"subitem_description":"研究報告書","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_publisher":"国吉正之","subitem_publisher_language":"ja"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/9307"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_source_identifier":"BA79259859","subitem_source_identifier_type":"NCID"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2006-06","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"none"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"16550042-3.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2004454/files/16550042-3.pdf"},"version_id":"9eab3f7f-3683-4312-8beb-26828a82885f"},{"accessrole":"open_access","filename":"16550042-2.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2004454/files/16550042-2.pdf"},"version_id":"74939a54-c4f6-4d83-a397-6e141729660f"},{"accessrole":"open_access","filename":"16550042-1.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2004454/files/16550042-1.pdf"},"version_id":"c9052261-224b-4152-86a3-a6ae804334a5"}]},"item_title":"沖縄産海藻類の化学的研究","item_type_id":"15","owner":"1","path":["1642838403123","1642838406414"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-03-19"},"publish_date":"2009-03-19","publish_status":"0","recid":"2004454","relation_version_is_last":true,"title":["沖縄産海藻類の化学的研究"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2024-11-19T07:05:35.487781+00:00"}