WEKO3
アイテム
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We studied coral metabolism using a Continuous Flow Complete Mixing (CFCM) experimental system. The CFCM experimental system is a newly developed experimental system for quantitative investigation of corals\u0027 metabolic changes. Coral incubation conditions tested were: 1) no addition of hydrogen peroxide (H_2O_2) to the supplied seawater (H_20_2 0 \u03bcM for 12 days) and 2) addition of various concentrations of H_2O_2 to the supplied seawater (0, 0.3, 3 and 30 \u03bcM H_2O_2 for 3 days each). With no addition of H_2O_2, the coral metabolisms (photosynthesis and calcification) were relatively stable, and we did not find any significant metabolic changes. The results suggested that when H_2O_2 was not added to the CFCM system, the coral did no suffer significant stress from the experimental system over a 12-day incubation period. When H_2O_2 concentrations were increased, large decreases in photosynthesis and calcification were observed. We found statistically significant differences in the photosynthesis rates between 0 nM and 3000, and 30000 nM H_2O_2. Additionally, we found statistically significant differences in the calcification rates between 0 and 30000 nM H_2O_2. 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サンゴ代謝に影響する環境ストレスの定量的解析
http://hdl.handle.net/20.500.12000/9308
http://hdl.handle.net/20.500.12000/930845220d24-cf79-4365-8faf-dbd28f1f2913
| 名前 / ファイル | ライセンス | アクション | |
|---|---|---|---|
17510013.pdf
|
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| Item type | デフォルトアイテムタイプ（フル）(1) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 公開日 | 2009-03-19 | |||||||||
| タイトル | ||||||||||
| タイトル | サンゴ代謝に影響する環境ストレスの定量的解析 | |||||||||
| 言語 | ja | |||||||||
| 作成者 |
新垣, 雄光
× 新垣, 雄光
× Arakaki, Takemitsu
|
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| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| 主題 | ||||||||||
| 言語 | ja | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | サンゴ | |||||||||
| 言語 | ja | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | 白化現象 | |||||||||
| 言語 | ja | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | 過酸化水素 | |||||||||
| 言語 | ja | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | 代謝 | |||||||||
| 言語 | ja | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | 活性酸素 | |||||||||
| 言語 | ja | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | 沖縄 | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 科研費番号: 17510013 | |||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 平成17年度～平成18年度科学研究費補助金基盤研究(C) 研究成果報告書 | |||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 研究概要 : 高温海水,紫外線,塩分変化などがサンゴに悪影響を及ぼし,白化させるストレス因子であることが知られている。しかし,各ストレス因子がサンゴに与える影響を定量的に解析した研究例は少ない。先の科学研究費補助金(若手研究(A))で,サンゴを長期間培養し,化学種の取り込み速度や放出速度を数値解析することができる実験システム(連続流水混合実験システム)の開発に成功した。本研究では,この実験システムを応用し,活性酸素種の一つである過酸化水素や海水温度などの環境ストレス因子がサンゴの生理活性に与える影響を定量的に解析することを目的とし,研究を実施した。実験では供給海水の過酸化水素濃度を0, 300, 3000, 30000nMと4段階に調整し,それぞれ3日間サンゴに供給した。その際,水温は一定,光量は12時間周期でON/OFFにした。研究の結果,過酸化水素0nM添加時と比べ,30000nM添加時で,石灰化の減少量は22.1-52.0%,光合成の減少量は18.6-48.0%であった。これより過酸化水素濃度上昇時,サンゴによる光合成及び石灰化が大きく阻害されることが明らかとなった。現在の沖縄本島周辺海域における過酸化水素濃度は最大で 300nM程度であるが,これ以上過酸化水素濃度が上昇した場合,サンゴの生育に大きく影響する可能性が高いと考えられる。また,沖縄の大気中には活性酸素種が多く含まれているが,海水への溶解はほとんど起こらないこと,海水中の過酸化水素濃度は,その場での光化学反応に大きく影響されることなどが明らかとなった。沿岸域では,干潮時にタイドプールができやすいため,そこでの活性酸素濃度の増加が懸念される。今後,さらに,研究を進め,タイドプールでの栄養塩濃度やその影響について調べる必要がある。 | |||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | Coral metabolism can be used as an indicator for physiological conditions of a coral colony. We studied coral metabolism using a Continuous Flow Complete Mixing (CFCM) experimental system. The CFCM experimental system is a newly developed experimental system for quantitative investigation of corals' metabolic changes. Coral incubation conditions tested were: 1) no addition of hydrogen peroxide (H_2O_2) to the supplied seawater (H_20_2 0 μM for 12 days) and 2) addition of various concentrations of H_2O_2 to the supplied seawater (0, 0.3, 3 and 30 μM H_2O_2 for 3 days each). With no addition of H_2O_2, the coral metabolisms (photosynthesis and calcification) were relatively stable, and we did not find any significant metabolic changes. The results suggested that when H_2O_2 was not added to the CFCM system, the coral did no suffer significant stress from the experimental system over a 12-day incubation period. When H_2O_2 concentrations were increased, large decreases in photosynthesis and calcification were observed. We found statistically significant differences in the photosynthesis rates between 0 nM and 3000, and 30000 nM H_2O_2. Additionally, we found statistically significant differences in the calcification rates between 0 and 30000 nM H_2O_2. Thus, incubation experiment we conduced suggested that higher H_2O_2 concentrations clearly influenced coral metabolisms. | |||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 未公開：P.66～92（論文別刷のため） | |||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 研究報告書 | |||||||||
| 出版者 | ||||||||||
| 言語 | ja | |||||||||
| 出版者 | 新垣雄光 | |||||||||
| 言語 | ||||||||||
| 言語 | jpn | |||||||||
| 資源タイプ | ||||||||||
| 資源タイプ | research report | |||||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_18ws | |||||||||
| 出版タイプ | ||||||||||
| 出版タイプ | VoR | |||||||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||||||
| 識別子 | ||||||||||
| 識別子 | http://hdl.handle.net/20.500.12000/9308 | |||||||||
| 識別子タイプ | HDL | |||||||||
| 収録物識別子 | ||||||||||
| 収録物識別子タイプ | NCID | |||||||||
| 収録物識別子 | BA82700482 | |||||||||
| 書誌情報 |
p. none, 発行日 2007-03 |
|||||||||
