@article{oai:u-ryukyu.repo.nii.ac.jp:02004591,
 author = {石橋, 治 and 西島, 拓 and 角田, かおり and 須藤, 健二 and 山下, 勝弘 and 小倉, 剛 and 砂川, 勝徳 and 仲田, 正 and Ishibashi, Osamu and Nishijima, Taku and Sumida, Kaori and Sudo, Kenji and Yamashita, Katsuhiro and Ogura, Go and Sunagawa, Katsunori and Nakada, Tadashi},
 issue = {55},
 journal = {琉球大学農学部学術報告, The Science Bulletin of the Faculty of Agriculture. University of the Ryukyus},
 month = {Dec},
 note = {We tried to the immunological experiment of antibody response with small Asian mongoose (Hepestes javanicus) captured on Okinawajima island, which inoculated plague (Yersinia pestis) vaccine, and then carried out the serological test measuring anti-plague antibody titer by IgG-ELISA. Three groups (each of four individuals) of mongoose inoculated by the different ways which in subcutaneous on their back, intradermal on their back, or in the crural muscle, respectively, and another three groups of mongoose inoculated with undiluted plague vaccine, diluted one at 1:10, or 1:10 with freund's adjuvant, respectively were compared. As the result, the group of mongoose inoculated in intradermal was found to increase of anti-plague antibody titer, and inoculated in diluted at 1:10 with adjuvant was found to higbIy increase of anti-plague antibody titer. The cutoff value (the average OD value + 3×standard deviation) calculated from negative control sera was 0.46, and the ratio of individual over the cutoff value was 5.6% (13 out of 231) by IgG-ELISA., ジャワマングースに対しペストF1抗原を負荷する免疫実験を行い，ペスト抗体産生の推移をIgG-ELISA法により，陽性および陰性の判断基準を求めた．その結果，免疫負荷は皮内接種により明確な抗体価の上昇を確認し，また，カットオフ値を0.46とした．次に抗-フェレットIgGを用いて，ペスト特異タンパク質F1を抗原として沖縄島のジャワマングースについて，2002年11月から2004年10月に捕獲された231頭における抗体保有調査を行った．カットオフ値を超える個体は5.2％であった．, 紀要論文},
 pages = {1--5},
 title = {沖縄島産マングースを用いたペスト抗体産生実験およびペスト抗体保有調査},
 year = {2008}
}