{"created":"2022-01-27T08:09:58.406397+00:00","id":2004593,"links":{},"metadata":{"_buckets":{"deposit":"710e28f5-8301-4781-acfe-38291b2b28a7"},"_deposit":{"id":"2004593","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2004593"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02004593","sets":["1642837622505:1642837905044:1642837925225","1642838403551:1642838407312"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"\u30e4\u30a8\u30e4\u30de\u30d2\u30eb\u30ae\u6a39\u76ae\u306e\u30e1\u30bf\u30ce\u30fc\u30eb\u62bd\u51fa\u753b\u5206\u306e\u751f\u7406\u6d3b\u6027\u3000\u4e00\u7d30\u80de\u6bd2\u6027\uff0c\u6297\u816b\u760d\u6d3b\u6027\u3092\u4e2d\u5fc3\u3068\u3057\u3066\uff0d","subitem_1551255648112":"ja"},{"subitem_1551255647225":"Physiological activities of methanol extracts from the bark of Rhizophora stylosa Griff. - On cytotoxicity and anti-tumor activity -","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"\u4e0a\u5730, \u4fca\u5fb3","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"\u9edb, \u5b8f\u548c","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"\u9ad8\u6a4b, \u68a2","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"\u91ce\u539f, \u654f\u6b21","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"\u91d1\u57ce, \u4e00\u5f66","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Uechi, Shuntoku","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Mayuzumi, Hirokazu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Takahashi, Kozue","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Nohara, Toshitsugu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kinjo, Kazuhiko","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"\u30e4\u30a8\u30e4\u30de\u30d2\u30eb\u30ae"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"ICR\u62c5\u764c\u30de\u30a6\u30b9"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"\u6297\u816b\u760d\u6d3b\u6027"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Rhizophora stylosa Griff"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"tumor-bearing"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"ICR mice"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Anti-tumor activity"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"The anti-tumor and other physiological activities of an extract of the bark of Rhizophora stylosa Griff obtained from Iriomotejima Island were investigated in vitro and in vivo. The water-soluble fraction of a methanol extract obtained from air-dried bark was separated by liquid-liquid partition using ethyl acetate. The ethyl acetate fraction was then separated by Sephadex LH-20 column chromatography to obtain methanol:water and acetone:water fractions. We then investigated the cytotoxicity of the four fractions in RAW264.7 cells. Survival rates at the lowest doses of all samples were very high. The effects of three different doses of these fractions on the NO production in LPS-treated RAW264.7 cells were investigated. The cells produced 140 \u03bcmol/L of NO in the presence of 40 \u03bcg/ml of the ethyl acetate fraction, which was about 1.6-fold more than that produced by control cells incubated with PBS. The ethyl acetate fraction was eluted once again through Sephadex LH-20 using ethanol/methanol, which generated 10 fractions (Fr.1 through 10). Murine peritoneal macrophages produced more NO when incubated with these fractions than with standard catechin. The ethyl acetate fraction (3 mg/day/mouse) was also administered intraperitoneally to ICR mice bearing sarcoma-180 tumors every other day for about 10 weeks. Although the survival of the mice did not significantly increase, tumor metastasis and volume tended to be suppressed. The ethyl acetate fraction of Rhizophora stylosa Griff increased NO production in RAW264.7 cells and in murine peritoneal macrophages, and suppressed the metastasis and proliferation of sarcoma-180 tumor cells. These findings suggest that this fraction contains useful immunoregulators.","subitem_description_type":"Other"},{"subitem_description":"\u897f\u8868\u5cf6\u7523\u30e4\u30a8\u30e4\u30de\u30d2\u30eb\u30ae\uff08Rhizophora stylosa Griff\uff09\u306e\u6a39\u76ae\u62bd\u51fa\u753b\u5206\u306b\u3064\u3044\u3066\uff0c\u6297\u816b\u760d\u6d3b\u6027\u7b49\u3092 in vitro \u304a\u3088\u3073in vivo \u3067\u691c\u8a0e\u3057\uff0c\u4ee5\u4e0b\u306e\u6210\u7e3e\u3092\u5f97\u305f\uff0e\u98a8\u4e7e\u6a39\u76ae\u304b\u3089\u30e1\u30bf\u30ce\u30fc\u30eb\u62bd\u51fa\u7269\u3092\u5f97\uff0c\u3053\u308c\u306e\u6c34\u53ef\u6eb6\u753b\u5206\u3092\u9162\u9178\u30a8\u30c1\u30eb\u3067\u6db2\u30fb\u6db2\u5206\u914d\u3057\u3066\u9162\u9178\u30a8\u30c1\u30eb\u753b\u5206\u3092\u5f97\u305f\uff0e\u3055\u3089\u306b\u9162\u9178\u30a8\u30c1\u30eb\u753b\u5206\u304b\u3089 Sephadex LH-20 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University of the Ryukyus"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueNumber":"55","bibliographicPageEnd":"15","bibliographicPageStart":"11"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"No55p011.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2004593/files/No55p011.pdf"},"version_id":"f770e801-2ba0-4b43-b950-6c213c467526"}]},"item_title":"\u30e4\u30a8\u30e4\u30de\u30d2\u30eb\u30ae\u6a39\u76ae\u306e\u30e1\u30bf\u30ce\u30fc\u30eb\u62bd\u51fa\u753b\u5206\u306e\u751f\u7406\u6d3b\u6027\u3000\u4e00\u7d30\u80de\u6bd2\u6027\uff0c\u6297\u816b\u760d\u6d3b\u6027\u3092\u4e2d\u5fc3\u3068\u3057\u3066\uff0d","item_type_id":"15","owner":"1","path":["1642837925225","1642838407312"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-04-22"},"publish_date":"2009-04-22","publish_status":"0","recid":"2004593","relation_version_is_last":true,"title":["\u30e4\u30a8\u30e4\u30de\u30d2\u30eb\u30ae\u6a39\u76ae\u306e\u30e1\u30bf\u30ce\u30fc\u30eb\u62bd\u51fa\u753b\u5206\u306e\u751f\u7406\u6d3b\u6027\u3000\u4e00\u7d30\u80de\u6bd2\u6027\uff0c\u6297\u816b\u760d\u6d3b\u6027\u3092\u4e2d\u5fc3\u3068\u3057\u3066\uff0d"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-02-15T04:47:39.225830+00:00"}