{"created":"2022-01-27T08:14:29.177574+00:00","id":2004750,"links":{},"metadata":{"_buckets":{"deposit":"639d6747-042d-4c43-85f0-35a7159c1dfb"},"_deposit":{"id":"2004750","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2004750"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02004750","sets":["1642838403123","1642838403551:1642838406414"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"イシサンゴにおける組織適合性の発現とその遺伝的制御","subitem_1551255648112":"ja"},{"subitem_1551255647225":"DEVELOPMENT AND GENETIC CONTROL OF HISTOINCOMPATIBILITY IN SCLERACTINIAN CORALS.","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"日高, 道雄","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Hidaka, Michio","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"組織適合性"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"イシサンゴ"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"キメラ"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"アロザイム"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"自他認識"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"刺胞動物"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"ALLOZYME"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"サンゴ"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"CHIMERA"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"HISTOCOMPATIBILITY"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"CORALS"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"科研費番号: 04804063","subitem_description_type":"Other"},{"subitem_description":"平成4年度～5年度科学研究費補助金（一般研究C）研究成果報告書","subitem_description_type":"Other"},{"subitem_description":"研究概要：本研究は、イシサンゴにおいて機能的な組織適合性がいつ発現するのかを明らかにすることを主な目的とした。ハナヤサイサンゴの幼群体間の接触反応を観察し、組織非適合性反応が時間とともに変化するかを調べるとともに、アザミサンゴの4つの色彩変異型を用いて接触実験を行い、得られたキメラ群体の性質を調べることを試みた。ハナヤサイサンゴでは、癒合、非癒合、非適合癒合の3つの接触反応が見られた。非適合癒合は、組織は連続しているものの、境界部に共生藻の少ない白い帯域が見られるという点で通常の癒合とは異なっていた。さらに非適合癒合を示すペアーの骨格は連続しているものの、境界部に溝がありその両側に突起状構造が向き合うように並んでいた。非適合癒合を示すペアーでは、境界部の白い帯域の拡大に伴い一方または両方の幼群体のポリプが吸収されることが観察された。非適合癒合を示したペア-の多くは、2～8カ月後に非癒合へと移行した。この場合境界部の両端から骨格に切れ込みが入り、両方の幼群体が間に骨格の壁をつくることにより非癒合へと移行した。アザミサンゴの異なる色彩を示すポリプ間においても、癒合の起こる場合があり、癒合により形成されたキメラ群体は安定で、約6カ月の観察期間中に2つに分離することはなかった。キメラ群体の遺伝的組成を調べるために、アロザイムの電気泳動を試みた。共生藻だけを集めて超音波処理して抽出した場合は、サンゴをホモゲナイズした場合と異なるバンドを示したので、今回用いた試料破砕法ではサンゴの酵素のみが抽出されたと考えられる。キメラ群体を構成した群体について、18種の酵素について電気泳動を行ったが、そのうちの8種でのみ解釈可能なバンドが得られた。しかし今回キメラ群体を形成したペアーは、これら8種の酵素について同一の電気泳動パターンを示したため、キメラ群体の遺伝組成を調べることはできなかった。","subitem_description_type":"Other"},{"subitem_description":"研究概要：Contact responses between primary polyps or young colonies derived from different colonies of the coral Pocillopora damicornis were examined. Polyps from the same colonies fused with each other. Polyps from different colonies showed either fusion, nonfusion, or incompatible fusion. In non-fused pairs, tissue was discontinuous and a skeletal ridge was formed at the interface. In incompatible fusion, tissues of the paired polyps appeared continuous but a white zone without zooxanthellae was observed at the interface of the polyps. Newly formed polyps located near the white zone sometimes disappeared or were partially resorbed as the white zone widened. When the skeleton of the polyps was observed with a SEM, the skeleton was continuous but a groove with skeletal spines on both sides was observed at the interface. Most of the polyps which showed incompatible fusion became separated by a skeletal ridge as in non-fused pairs or by a narrow zone of skeleton without living tissue after 2 - 8 months. The incompatible fusion might be a histoincompatible response which may later transform into nonfusion response. Polyps isolated from different colonies of Galaxa fascicularis were brought into contact. Polyps isolated from colonies belonging to different color morphs generally did not fuse with each other. However, in one combination (Gt-Gs), Tissue appeared continuous but a white zone with no or only a few zooxanthellae was observed at the interface. To examine genetic nature of the chimeric colonies produced by fusion of polyps belonging to different coloe morphs, I attempted to examine the allozyme pattern of constituents of the chimeric colonies. Eighteen enzymes were stained but only 8 enzymes produced interpretable bands. Since constituents of the chimeric colonies displayed the same zymographs, I could not analyze the genetic nature of the chimeras.","subitem_description_type":"Other"},{"subitem_description":"研究報告書","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"日高道雄"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"jpn"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/11488"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"NCID","subitem_1522646572813":"BA63294453"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994-03","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"none"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"04804063-3.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2004750/files/04804063-3.pdf"},"version_id":"dc6338e6-ebf3-4e60-b8aa-9238ba98197a"},{"accessrole":"open_access","filename":"04804063-2.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2004750/files/04804063-2.pdf"},"version_id":"57ddeb59-9a20-4b74-89dd-f7e7109e3ef9"},{"accessrole":"open_access","filename":"04804063-1.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2004750/files/04804063-1.pdf"},"version_id":"081fe533-ac58-458f-9c29-a15f79adfb4d"}]},"item_title":"イシサンゴにおける組織適合性の発現とその遺伝的制御","item_type_id":"15","owner":"1","path":["1642838403123","1642838406414"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-07-27"},"publish_date":"2009-07-27","publish_status":"0","recid":"2004750","relation_version_is_last":true,"title":["イシサンゴにおける組織適合性の発現とその遺伝的制御"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T02:03:56.401197+00:00"}