{"created":"2022-01-27T08:14:44.571429+00:00","id":2004759,"links":{},"metadata":{"_buckets":{"deposit":"264445ad-ec72-4789-b9de-9e149dd22d5a"},"_deposit":{"id":"2004759","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2004759"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02004759","sets":["1642838403123","1642838403551:1642838406414"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"サンゴ-褐虫藻共生系の多様性とストレス耐性に関する研究","subitem_1551255648112":"ja"},{"subitem_1551255647225":"Studies on diversity and stress tolerance of coral-zooxanthella symbiotic system.","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"日高, 道雄","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"広瀬, 慎美子","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"磯村, 尚子","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Hidaka, Michio","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Hirose, Mamiko","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Isomura, Naoko","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"ストレス応答"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"高温ストレス"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"共生"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"サンゴ"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"褐虫藻"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"光化学系"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"活性酸素"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"白化"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"stress response"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"photochemical system"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"active oxygen"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"thermal stress"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"bleaching"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"coral"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"zooxanthellae"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"symbiosis"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"科研費番号: 17570023","subitem_description_type":"Other"},{"subitem_description":"平成17年度～平成18年度科学研究費補助金(基盤研究C)研究成果報告書","subitem_description_type":"Other"},{"subitem_description":"研究概要：本研究では、サンゴ-褐虫藻間の共生関係の多様性と共生体のストレス耐性の関係を明らかにすること、そして白化の機構を解明することを主な目的とし、下記の実験を行った。造礁サンゴ2種(トガリシコロサンゴPavona divaricataおよびクサビライシFungia sp.)の解離細胞より細胞凝集塊(tissue ball)を調整し、白化機構を調べるための実験系を開発した。両種について、高温ストレス下では、褐虫藻密度とtissue ballの生存期間の間に負の相関関係があることを見出した。このことは、ストレス下で褐虫藻が共生体にとって有害な物質を産生していることを示唆する。高温ストレスによるtissue ball生存期間の短縮が活性酸素除去剤により緩和されることから、この有害物質は活性酸素であることが示唆された。シワリュウモンサンゴPachyseris rugosaを暗黒下で1時間高温処理すると、PSIIの最大量子効率(Fv/Fm)は変化しなかったが、炭素固定回路機能の指標である最大霊子伝達速度(ETRmax)は有意な、しかし回復可能な低下を示した。一方Fv/Fmの少なくとも60%が機能していれば最大電子伝達速度は維持されることから、高温ストレスによる褐虫藻光合成系の損傷は、最初にPSII以降の要素、おそらく炭素固定回路、に生じ、PSIIの損傷は2次的なものであることが示唆された。トゲスギミドリイシAcropora nobilisおよびニオウミドリイシAcropora paliferaの幼生または一次ポリプが、異なる宿主由来の褐虫藻(クレードA, B, C, D)を取り込み、共生系を樹立することを見いだした。今後このシステムを用いて、褐虫藻の遺伝子型、生理学的性質の差異が共生体のストレス耐性にどのような影響を及ぼすかを調べることが可能となった。","subitem_description_type":"Other"},{"subitem_description":"To investigate bleaching mechanisms of coral-zooxanthella symbiotic system at cellular or tissue level, we have established an experimental system using coral cell aggregates (tissue balls). Tissue balls prepared from dissociated cells of Fungia sp. and Pavona divaricata were exposed to either 31°C or 25°C. Survival curves of tissue balls were markedly different between 31°C and 25°C. There was a significant negative correlation between the survival time and zooxanthella density of tissue balls at 31°C, while no significant relationship was found at 25°C. The present results showed that zooxanthellae produced harmful substances and cause damage to coral ce Is under high temperature stress. The finding that the antioxidants extended the survival time of tissue balls at high temperature suggests that zooxanthellae produce reactive oxygen species under the stress condition. To examine whether damage to zooxanthellar photosystem II (PSII) is the primary step of thermally induced coral bleaching, we assessed the relationship between the maximum quantum yield of PSII (Fv/Fm) or active PSII centers (Fv/Fo) and maximum electron transport rate (ETRmax) in the symbiotic algae of the coral Pachyseris rugosa. The I h high temperature treatment in darkness produced a significant, but reversible decline in ETRmax without any change in Fv/Fm. A minimum of 60% of the Fv/Fm or 30% of active PS II centers (Fv/Fo) is required to maintain ETRmax in the symbionts. These results suggest that the primary step of heat-induced damage in the symbiont's photosynthetic apparatus involves a component beyond the PSII, probably at the level of the dark reaction as indicated by reduced ETRmax and the PSII damage is secondary. Planulae or primary polyps of Acropora nobilis and A. palifera could be infected with zooxanthellae from various hosts. We are planning to use this experimental system to assess effects of environmental stresses on corals associated with different types of zooxanthllae.","subitem_description_type":"Other"},{"subitem_description":"研究報告書","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"日高道雄"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"jpn"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/11528"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"NCID","subitem_1522646572813":"BA82748252"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"9999-01-01","bibliographicIssueDateType":"Issued"},"bibliographicPageStart":"none"}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"17570023.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2004759/files/17570023.pdf"},"version_id":"184bfad0-4a9b-4e94-a1c8-fb98f81f07e0"}]},"item_title":"サンゴ-褐虫藻共生系の多様性とストレス耐性に関する研究","item_type_id":"15","owner":"1","path":["1642838403123","1642838406414"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-07-28"},"publish_date":"2009-07-28","publish_status":"0","recid":"2004759","relation_version_is_last":true,"title":["サンゴ-褐虫藻共生系の多様性とストレス耐性に関する研究"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T02:04:06.954562+00:00"}