{"created":"2022-01-28T01:01:06.541316+00:00","id":2005084,"links":{},"metadata":{"_buckets":{"deposit":"594c2212-e014-4c44-9169-b3b6f3828642"},"_deposit":{"id":"2005084","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2005084"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02005084","sets":["1642838403123","1642838403551:1642838407795"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"B型、D型肝炎ウイルス重感染患者の長期予後とそれに影響を与える因子の解明","subitem_1551255648112":"ja"},{"subitem_1551255647225":"Long-term outcome of patients co-infected with hepatitis B and D virus","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"外間, 昭","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"比嘉, 太","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Hokama, Akira","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Higa, Futoshi","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"B型肝炎ウイルス"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"D型肝炎ウイルス"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"慢性肝炎"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"肝癌"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"分子生物学"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"遺伝子型"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"長期予後"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"疫学調査"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"hepatitis B virus"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"hepatitis D virus"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"chronic hepatitis"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"hepatocellular carcinoma"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"molecular biology"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"genotype"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"long-term outcome"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"epidemiological study"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"科研費番号: 16590608","subitem_description_type":"Other"},{"subitem_description":"平成16年度～平成18年度科学研究費補助金(基盤研究(C))研究成果報告書","subitem_description_type":"Other"},{"subitem_description":"研究概要：沖縄県宮古群島の一離島の住民を対象に1994年から、B型とD型肝炎の調査を行っている。これまでに2575名が調査され、そのうちB型肝炎ウイルス(HBV)の保有者は236名(9.2%)で、これらの保有者のうち49名(21.2%)がHBVとD型肝炎ウイルス(HDV)の重感染者であった。また、同群島内にある総合病院の受診者を対象とした調査で、HDVの重感染は肝硬変、肝癌への進行に関連していることが判った。HDVは、これまでに世界中から多数の株が分離され、これらの株間には多様性が大きい。そこで、株間で相同性の高い領域にプライマーを設定し、独自のPCRを開発した。これを用いて、HDV抗体陽性者を検査したところ、約半数はHDV-RNAが陽性で、残りは陰性であった。また、HDV-RNAが陽性者は、高率に肝障害を有したのに反し、陰性者は肝障害がなかった。よって、HDVは感染後、HBVが存在するにもかかわらず、排除される症例が存在することが判った。また、HDVの定量法をリアルタイムPCR法にて開発し、病態との関連を検討した。その結果、HBVとHDVの重感染の肝障害は、主にHDV量と関連することが判った。HDVはその系統樹解析から、大きく3つのタイプ(I、II、III型)に分類される。宮古群島で見つかったHDVはほとんどがII型に属し、しかもIIbと亜分類された。IIb型は台湾からも報告されているが、宮古群島で見つかったHDV株はより多様性があり、また、その多様性が病態にも影響していると思われた。また、HBVは95%がgenotype Bで、残り5%がgenotype Cであった。","subitem_description_type":"Other"},{"subitem_description":"要約（欧文）:To clarify the correlation between HBV DNA levels and serum ALT levels in patients with chronic hepatitis HDV infection, sensitive HBV quantitative assays were used. Thirty-four consecutive patients with CLD who were positive for both HBsAg and anti-HDV, including 19 patients with CH, 8 patients with LC and 7 patients with HCC. All were negative for HBeAg and positive for antibody to HBeAg. HBV DNA was detected in 25 (73.5%) of the 34 patients using real-time detection PCR, and the HBV DNA levels of these patients were significantly lower compared with HBeAg status and ALT level-matched patients with CLD positive for HBsAg but negative for anti-HDV. There was no correlation between serum HBV DNA and ALT levels among the 34 patients with CLD positive for anti-HDV. ALT levels in anti-HDV-positive HBsAg carriers with HDV RNA were significantly higher than those without HDV RNA. Liver damage in patients with HDV infection may be caused mainly by ongoing HDV infection not by HBV replication. We determined the sequence of HDV genome in 40 patients from the Miyako Islands, Okinawa. Consensus sequences from 33 HDV full genomes were determined by directly sequencing four partially overlapping PCR products. Phylogenetic tree analysis classified these 33 complete HDV genomes as HDV genotype I, IIa and IIb. Among the 30 genotype IIb patients, there were two clusters of genetic variants. One group consisted of six isolates showing significant homology with genotype IIb, previously reported from Taiwan. The other group consisted of 24 isolates, whose sequences formed a new genetic subgroup (genotype IIb-Miyako; IIb-M). Characteristic variations were found in the C-terminal sequence of the large delta antigen-conferring packaging signal as well as the RNA editing site. Determination of subclasses of genotype IIb, including HDV patients whose partial HDV sequence was determined, revealed eight patients with IIb and 29 patients with IIb-M. Patients with genotype IIb-M showed greater progression of CH and LC than those with genotype IIb. These data indicate the existence of a genetic subgroup of HDV genotype IIb, which is associated with different clinical characteristics and which could be related to genetic variations in functionally important parts of the HDV genome.","subitem_description_type":"Other"},{"subitem_description":"未公開：P.4以降（別刷論文のため）","subitem_description_type":"Other"},{"subitem_description":"研究報告書","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"外間昭"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/13627"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"NCID","subitem_1522646572813":"BA82738689"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2007-05","bibliographicIssueDateType":"Issued"}}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"16590608.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2005084/files/16590608.pdf"},"version_id":"b4fedd8a-3c3a-4581-b1fd-38c7c72560c4"}]},"item_title":"B型、D型肝炎ウイルス重感染患者の長期予後とそれに影響を与える因子の解明","item_type_id":"15","owner":"1","path":["1642838403123","1642838407795"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-12-04"},"publish_date":"2009-12-04","publish_status":"0","recid":"2005084","relation_version_is_last":true,"title":["B型、D型肝炎ウイルス重感染患者の長期予後とそれに影響を与える因子の解明"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T02:29:06.463737+00:00"}