{"_buckets": {"deposit": "bfe010c6-0406-4781-ae13-d3bf00cb1d4b"}, "_deposit": {"id": "2005129", "owners": [1], "pid": {"revision_id": 0, "type": "depid", "value": "2005129"}, "status": "published"}, "_oai": {"id": "oai:u-ryukyu.repo.nii.ac.jp:02005129", "sets": ["1642838403123", "1642838407312"]}, "author_link": [], "item_1617186331708": {"attribute_name": "Title", "attribute_value_mlt": [{"subitem_1551255647225": "\u30d6\u30bf\u4f53\u5916\u6210\u719f\u5375\u5b50\u306e\u4f53\u5916\u53d7\u7cbe\u6642\u306b\u304a\u3051\u308b\u591a\u7cbe\u5b50\u53d7\u7cbe\u6291\u5236\u306b\u95a2\u308f\u308b\u56e0\u5b50\u306e\u89e3\u660e", "subitem_1551255648112": "ja"}]}, "item_1617186419668": {"attribute_name": "Creator", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u5efa\u672c, \u79c0\u6a39", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "\u4ef2\u7530, \u6b63", "creatorNameLang": "ja"}]}, {"creatorNames": [{"creatorName": "Tatemoto, Hideki", "creatorNameLang": "en"}]}, {"creatorNames": [{"creatorName": "Nakada, Tadashi", "creatorNameLang": "en"}]}]}, "item_1617186476635": {"attribute_name": "Access Rights", "attribute_value_mlt": [{"subitem_1522299639480": "open access", "subitem_1600958577026": "http://purl.org/coar/access_right/c_abf2"}]}, "item_1617186609386": {"attribute_name": "Subject", "attribute_value_mlt": [{"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u30d6\u30bf\u5375\u5b50"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u900f\u660e\u5e2f"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u30ec\u30af\u30c1\u30f3"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u7cd6\u9396\u672b\u7aef"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u591a\u7cbe\u5b50\u4fb5\u5165"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u7cd6\u30bf\u30f3\u30d1\u30af\u8cea"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u786c\u5316"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u591a\u7cbe\u5b50\u53d7\u7cbe"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u4f53\u5916\u53d7\u7cbe"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u6ca1\u98df\u5b50\u9178"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u30bf\u30f3\u30cb\u30f3\u9178"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u30bf\u30f3\u30cb\u30f3\u95a2\u9023\u5316\u5408\u7269"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u30d2\u30a2\u30eb\u30ed\u30cb\u30c0\u30fc\u30bc\u6d3b\u6027"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u30d6\u30bf\u7cbe\u5b50"}, {"subitem_1522299896455": "ja", "subitem_1522300014469": "Other", "subitem_1523261968819": "\u30a8\u30e9\u30b0\u9178"}]}, "item_1617186626617": {"attribute_name": "Description", "attribute_value_mlt": [{"subitem_description": "\u79d1\u7814\u8cbb\u756a\u53f7: 17580247", "subitem_description_type": "Other"}, {"subitem_description": "\u5e73\u621017\u5e74\u5ea6\uff5e\u5e73\u621019\u5e74\u5ea6\u79d1\u5b66\u7814\u7a76\u8cbb\u88dc\u52a9\u91d1(\u57fa\u76e4\u7814\u7a76(C))\u7814\u7a76\u6210\u679c\u5831\u544a\u66f8", "subitem_description_type": "Other"}, {"subitem_description": "\u7814\u7a76\u6982\u8981:(\u5e73\u621018\u5e74\u5ea6\u6642\u70b9)\u3053\u308c\u307e\u3067\u306e\u6211\u3005\u306e\u7814\u7a76\u3067,\u7cbe\u5b50\u30d2\u30a2\u30eb\u30ed\u30cb\u30c0\u30fc\u30bc\u306b\u5bfe\u3059\u308b\u5f37\u3044\u6297\u30d2\u30a2\u30eb\u30ed\u30cb\u30c0\u30fc\u30bc\u52b9\u679c\u306f,\u900f\u660e\u5e2f\u3078\u7d50\u5408\u3057\u305f\u7cbe\u5b50\u3067\u5f15\u304d\u8d77\u3053\u3055\u308c\u308b\u5148\u4f53\u53cd\u5fdc\u3092\u6291\u5236\u3059\u308b\u4f5c\u7528\u6a5f\u5e8f\u3092\u4ecb\u3057\u3066\u30d6\u30bf\u4f53\u5916\u53d7\u7cbe\u6642\u306e\u591a\u7cbe\u5b50\u4fb5\u5165\u306e\u983b\u5ea6\u3092\u52b9\u679c\u7684\u306b\u6e1b\u5c11\u3055\u305b\u308b\u3053\u3068\u3092\u660e\u3089\u304b\u306b\u3057\u305f\u3002\u4e00\u65b9,\u30d6\u30bf\u5375\u5b50\u3067\u591a\u767a\u3059\u308b\u591a\u7cbe\u5b50\u4fb5\u5165\u306f,\u8868\u5c64\u9846\u7c92\u306e\u958b\u88c2\u306b\u4f34\u3063\u305f\u4e00\u9023\u306e\u900f\u660e\u5e2f(ZP)\u53cd\u5fdc\u306e\u4e0d\u5099\u306b\u8d77\u56e0\u3057\u3066\u3044\u308b\u3068\u601d\u308f\u308c\u308b\u3002\u305d\u3053\u3067\u672c\u7814\u7a76\u306f,\u4f53\u5916\u6210\u719f\u30d6\u30bf\u5375\u306b\u76f4\u6d41\u96fb\u6c17\u30d1\u30eb\u30b9\u523a\u6fc0(ES)\u3092\u4e0e\u3048,\u5375\u6d3b\u6027\u5316\u306b\u4f34\u3046ZP\u69cb\u6210\u7cd6\u30bf\u30f3\u30d1\u30af\u8cea(ZP1,ZP2,ZP3\u306a\u3089\u3073\u306bZP4)\u306e\u7cd6\u9396\u672b\u7aef\u306e\u5909\u5316\u3092\u8ffd\u7a76\u3057\u305f\u3002\u307e\u305a\u521d\u3081\u306b,ES\u51e6\u7406\u5375\u306b\u304a\u3051\u308b\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u6eb6\u89e3\u53cd\u5fdc\u3078\u306eZP\u62b5\u6297\u6027\u306a\u3089\u3073\u306bZP\u3078\u306e\u7d50\u5408\u7cbe\u5b50\u6570\u306e\u5909\u5316\u3092\u7d4c\u6642\u7684\u306b\u89b3\u5bdf\u3057\u305f\u3068\u3053\u308d,ES\u51e6\u74064\u6642\u9593\u5f8c\u306b\u900f\u660e\u5e2f\u306e\u786c\u5316\u304c\u8d77\u3053\u308a,\u6b21\u3044\u3067ES\u51e6\u740612\u6642\u9593\u304b\u308918\u6642\u9593\u306b\u639b\u3051\u3066\u7d50\u5408\u7cbe\u5b50\u6570\u306e\u8457\u3057\u3044\u6e1b\u5c11\u304c\u8a8d\u3081\u3089\u308c\u305f\u3002\u305d\u3053\u3067,\u30d6\u30bf\u306b\u304a\u3051\u308b\u7cbe\u5b50-ZP\u9593\u306e\u7d50\u5408\u306b\u95a2\u4e0e\u3059\u308b ZP\u69cb\u6210\u7cd6\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5858\u9396\u672b\u7aef\u306b\u5bfe\u3057\u3066\u7279\u7570\u7684\u306a\u89aa\u548c\u6027\u3092\u793a\u3059WGA, S-WGA\u304a\u3088\u3073RCA-1\u306e\u5404\u30ec\u30af\u30c1\u30f3\u3092\u7528\u3044,ZP\u69cb\u6210\u7cd6\u30bf\u30f3\u30d1\u30af\u8cea\u3078\u306e\u30ec\u30af\u30c1\u30f3\u7d50\u5408\u6027\u5909\u5316\u3092SDS-PAGE\u5f8c\u306eECL\u6cd5\u3067\u691c\u51fa\u3057\u305f\u3002\u305d\u306e\u7d50\u679c,GlcNAc\u672b\u7aef\u3092\u8a8d\u8b58\u3059\u308bWGA\u3068S-WGA\u306fZP2\u306a\u3089\u3073\u306bZP3\u3078\u7d50\u5408\u3057,\u3053\u308c\u3089\u7d50\u5408\u80fd\u306fES\u51e6\u740618\u6642\u9593\u4ee5\u964d\u306b\u6709\u610f\u306b\u4f4e\u4e0b\u3057\u305f\u3002\u4e00\u65b9,GalNAc\u672b\u7aef\u3092\u8a8d\u8b58\u3059\u308bRCA-1\u3067\u306f,ZP1,ZP2\u304a\u3088\u3073ZP3\u3078\u306e\u7d50\u5408\u304c\u691c\u51fa\u3055\u308c,ES\u51e6\u74064\u6642\u9593\u5f8c\u306bZP1\u3078\u306e\u7d50\u5408\u80fd\u304c,12\u6642\u9593\u5f8c\u306b\u306f ZP2\u3068ZP3\u3078\u306e\u7d50\u5408\u80fd\u3082\u6709\u610f\u306b\u4f4e\u4e0b\u3057\u305f\u3002\u3057\u304b\u3057,ES\u51e6\u740624\u6642\u9593\u5f8c\u306b\u306f\u518d\u3073RCA-1\u306b\u3088\u308bZP\u7cd6\u30bf\u30f3\u30d1\u30af\u8cea\u3078\u306e\u7d50\u5408\u80fd\u306f\u5897\u52a0\u3057\u305f\u3002\u4ee5\u4e0a\u306e\u7d50\u679c\u304b\u3089,\u30d6\u30bf\u5375\u3067\u306f\u6d3b\u6027\u5316\u306b\u4f34\u3044ZP\u306e\u786c\u5316\u306e\u307f\u306a\u3089\u305a,\u7cbe\u5b50\u3068\u306e\u7d50\u5408\u306b\u95a2\u308f\u308bZP\u69cb\u6210\u7cd6\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7cd6\u9396\u672b\u7aef\u3082\u975e\u5e38\u306b\u9045\u3044\u6bb5\u968e\u3067\u5909\u5316\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u660e\u3089\u304b\u3068\u306a\u3063\u305f\u3002", "subitem_description_type": "Other"}, {"subitem_description": "\u672a\u516c\u958b\uff1aP.6\u4ee5\u964d(\u5225\u5237\u8ad6\u6587\u306e\u305f\u3081)", "subitem_description_type": "Other"}, {"subitem_description": "\u7814\u7a76\u5831\u544a\u66f8", "subitem_description_type": "Other"}]}, "item_1617186643794": {"attribute_name": "Publisher", "attribute_value_mlt": [{"subitem_1522300295150": "ja", "subitem_1522300316516": "\u5efa\u672c\u79c0\u6a39"}]}, "item_1617186702042": {"attribute_name": "Language", "attribute_value_mlt": [{"subitem_1551255818386": "jpn"}]}, "item_1617186783814": {"attribute_name": "Identifier", "attribute_value_mlt": [{"subitem_identifier_type": "HDL", "subitem_identifier_uri": "http://hdl.handle.net/20.500.12000/13867"}]}, "item_1617186920753": {"attribute_name": "Source Identifier", "attribute_value_mlt": [{"subitem_1522646500366": "NCID", "subitem_1522646572813": "BA8643186X"}]}, "item_1617187056579": {"attribute_name": "Bibliographic Information", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2008-03", "bibliographicIssueDateType": "Issued"}}]}, "item_1617258105262": {"attribute_name": "Resource Type", "attribute_value_mlt": [{"resourcetype": "research report", "resourceuri": "http://purl.org/coar/resource_type/c_18ws"}]}, "item_1617265215918": {"attribute_name": "Version Type", "attribute_value_mlt": [{"subitem_1522305645492": "VoR", "subitem_1600292170262": "http://purl.org/coar/version/c_970fb48d4fbd8a85"}]}, "item_1617605131499": {"attribute_name": "File", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_access", "download_preview_message": "", "file_order": 0, "filename": "17580247.pdf", "future_date_message": "", "is_thumbnail": false, "mimetype": "", "size": 0, "url": {"objectType": "fulltext", "url": "https://u-ryukyu.repo.nii.ac.jp/record/2005129/files/17580247.pdf"}, "version_id": "b64c0cfd-a629-4883-98d0-669735b3fb2f"}]}, "item_title": "\u30d6\u30bf\u4f53\u5916\u6210\u719f\u5375\u5b50\u306e\u4f53\u5916\u53d7\u7cbe\u6642\u306b\u304a\u3051\u308b\u591a\u7cbe\u5b50\u53d7\u7cbe\u6291\u5236\u306b\u95a2\u308f\u308b\u56e0\u5b50\u306e\u89e3\u660e", "item_type_id": "15", "owner": "1", "path": ["1642838403123", "1642838407312"], "permalink_uri": "http://hdl.handle.net/20.500.12000/13867", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2009-12-11"}, "publish_date": "2009-12-11", "publish_status": "0", "recid": "2005129", "relation": {}, "relation_version_is_last": true, "title": ["\u30d6\u30bf\u4f53\u5916\u6210\u719f\u5375\u5b50\u306e\u4f53\u5916\u53d7\u7cbe\u6642\u306b\u304a\u3051\u308b\u591a\u7cbe\u5b50\u53d7\u7cbe\u6291\u5236\u306b\u95a2\u308f\u308b\u56e0\u5b50\u306e\u89e3\u660e"], "weko_shared_id": -1}