{"created":"2022-01-28T01:03:11.650198+00:00","id":2005149,"links":{},"metadata":{"_buckets":{"deposit":"011e7a06-eb96-42a5-8c3b-e3eeee2b80b2"},"_deposit":{"id":"2005149","owners":[1],"pid":{"revision_id":0,"type":"depid","value":"2005149"},"status":"published"},"_oai":{"id":"oai:u-ryukyu.repo.nii.ac.jp:02005149","sets":["1642838403123","1642838403551:1642838407795"]},"author_link":[],"item_1617186331708":{"attribute_name":"Title","attribute_value_mlt":[{"subitem_1551255647225":"電気痙攣療法による脊髄虚血耐性獲得に関する基礎研究","subitem_1551255648112":"ja"},{"subitem_1551255647225":"Research for ischemic tolerance in the spinal cord induced by the electrical convulsion therapy.","subitem_1551255648112":"en"}]},"item_1617186419668":{"attribute_name":"Creator","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"垣花, 学","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"原田, 秀樹","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"笹良, 剛史","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"川端, 徹也","creatorNameLang":"ja"}]},{"creatorNames":[{"creatorName":"Kakinohana, Manabu","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Harada, Hideki","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Sasara, Takeshi","creatorNameLang":"en"}]},{"creatorNames":[{"creatorName":"Kawabata, Tetsuya","creatorNameLang":"en"}]}]},"item_1617186476635":{"attribute_name":"Access Rights","attribute_value_mlt":[{"subitem_1522299639480":"open access","subitem_1600958577026":"http://purl.org/coar/access_right/c_abf2"}]},"item_1617186609386":{"attribute_name":"Subject","attribute_value_mlt":[{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"脊髄虚血"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"脊髄電気刺激療法"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"脊髄血流量"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"虚血耐性"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"対麻痺"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Spinal cord ischemia"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Electrical convulsion therapy"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Spinal blood flow"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Ishcemic Tolerance"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"Paraplegia"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"c-fos"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"ラット"},{"subitem_1522299896455":"en","subitem_1522300014469":"Other","subitem_1523261968819":"HSP"},{"subitem_1522299896455":"ja","subitem_1522300014469":"Other","subitem_1523261968819":"電気痙攣療法"}]},"item_1617186626617":{"attribute_name":"Description","attribute_value_mlt":[{"subitem_description":"科研費番号: 14571454","subitem_description_type":"Other"},{"subitem_description":"平成14年度～平成15年度科学研究費補助金(基盤研究(C)(2))研究成果報告書","subitem_description_type":"Other"},{"subitem_description":"研究概要:ラット大動脈遮断による脊髄虚血モデルを用い、脳・脊髄電気痙攣による虚血耐性に関する研究を行った。脳電気痙攣療法による痙攣を誘発することが困難であったため、脊髄硬膜外電気刺激による虚血耐性獲得に関する研究を行った。雄性SDラット(350－400g)を用い、硬膜外刺激電極(銀製)を挿入した。その5日後に、脊髄刺激電極より電気刺激(矩形波、5mA、10秒間)を与えた。虚血耐性は、急性タイプ(電気刺激後30分)と遅発タイプ(電気刺激後24時間)に関して実験した。脊髄虚血時間は、6分間から11分間(1分間隔)で行い、ZivinらのLogistic解析法により50％のラットが対麻痺を来たす虚血時間(P50)を求め、比較した。その結果、電気刺激30分後に脊髄虚血を与えた場合のP50は7.2分であり、電気刺激なし群 (P50＝7.6分)と有意差はみられなかった。一方、電気刺激24時間後に脊髄虚血を与えた場合のP50は9.1分であり、電気刺激なし群より有意に長くなった。また、電気刺激24時間後の大動脈遮断時の脊髄血流量をレーザー血流計を用いて測定したが、刺激群と刺激なし群では脊髄血流量に有意差が認められなかった。さらに、9分間の脊髄虚血における硬膜外電気痙攣療法の虚血耐性効果の検討を行ったところ、電気刺激30分後では脊髄虚血耐性は認められなかったが、電気刺激24時間後では有意に虚血耐性が認められた。以上のことから、脊髄電気痙攣療法により脊髄虚血耐性は認められたが、それは急性タイプではなく遅発タイプの耐性効果であった。さらにその耐性効果は脊髄血流量に依存するものではなく、おそらく蛋白合成(HSP,栄養因子など)に依存するものであることが推測された。","subitem_description_type":"Other"},{"subitem_description":"要約(欧文):This study examines whether electrical stimuli on the spinal cord is also capable of inducing ischemic tolerance to ischemic spinal cord injury induced by transient aortic occlusion. The pretreatment of epidural electrical-stimulation(5mA,10s) was administered via the epidural silver electrodes. Spinal cord ischemia (SCI) was induced by the placement and subsequent inflation of a 2F Fogarty catheter which was inserted the descending thoracic aorta and combined with systemic hypotension(40mmHg). Animals were divided into four groups employed as follows ; Rapid Preconditioning-rats implanted with the epidural electrodes were exposed 9min of aortic occlusion 30min after the sham pretreatment of epidural electrical-stimulation (group RC:n=8) or the pretreatment of epidural electrical-stimulation (group RE:n=8) under isoflurane anesthesia, Delayed Preconditioning-rats implanted with the epidural electrodes were exposed 9min of aortic occlusion,24 hours after the sham pretreatment of epidural electrical-stimulation (group DC:n=8) or the pretreatment of epidural electrical-stimulation(group DE:n=8) under isoflurane anesthesia. In addition, rats were exposed with 6 up to l11min of SCI at 24 hours after epidural electrical-stimulation or sham stimulation. The group P50 represents the duration of SCI associated with 50% probability of resultant paraplegia. Our results showed that pretreatment of electrical stimulation at 24h, but not 30min before ischemia, protect the spinal cord against ischemic insults by aortic occlusion and that this stimulation prolong P50 by a approximately 15.0% compared with the control group. Since there is no difference in the spinal cord blood flow, measured by laser flow meter, during aortic occlusion between the group DE and DC, the neuroprotective effect in the group DE is not caused by the increase of collateral flow in spinal cord.","subitem_description_type":"Other"},{"subitem_description":"未公開：P.4以降(別刷論文のため)","subitem_description_type":"Other"},{"subitem_description":"研究報告書","subitem_description_type":"Other"}]},"item_1617186643794":{"attribute_name":"Publisher","attribute_value_mlt":[{"subitem_1522300295150":"ja","subitem_1522300316516":"垣花学"}]},"item_1617186702042":{"attribute_name":"Language","attribute_value_mlt":[{"subitem_1551255818386":"eng"}]},"item_1617186783814":{"attribute_name":"Identifier","attribute_value_mlt":[{"subitem_identifier_type":"HDL","subitem_identifier_uri":"http://hdl.handle.net/20.500.12000/13988"}]},"item_1617186920753":{"attribute_name":"Source Identifier","attribute_value_mlt":[{"subitem_1522646500366":"NCID","subitem_1522646572813":"BA74845275"}]},"item_1617187056579":{"attribute_name":"Bibliographic Information","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2004-05","bibliographicIssueDateType":"Issued"}}]},"item_1617258105262":{"attribute_name":"Resource Type","attribute_value_mlt":[{"resourcetype":"research report","resourceuri":"http://purl.org/coar/resource_type/c_18ws"}]},"item_1617265215918":{"attribute_name":"Version Type","attribute_value_mlt":[{"subitem_1522305645492":"VoR","subitem_1600292170262":"http://purl.org/coar/version/c_970fb48d4fbd8a85"}]},"item_1617605131499":{"attribute_name":"File","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_access","filename":"14571454.pdf","mimetype":"application/pdf","url":{"objectType":"fulltext","url":"https://u-ryukyu.repo.nii.ac.jp/record/2005149/files/14571454.pdf"},"version_id":"a8dfd27d-0f88-4971-b945-6001ee0f2be5"}]},"item_title":"電気痙攣療法による脊髄虚血耐性獲得に関する基礎研究","item_type_id":"15","owner":"1","path":["1642838403123","1642838407795"],"pubdate":{"attribute_name":"PubDate","attribute_value":"2009-12-16"},"publish_date":"2009-12-16","publish_status":"0","recid":"2005149","relation_version_is_last":true,"title":["電気痙攣療法による脊髄虚血耐性獲得に関する基礎研究"],"weko_creator_id":"1","weko_shared_id":-1},"updated":"2022-10-31T02:30:35.379318+00:00"}